Summary of Study ST000001
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000001. The data can be accessed directly via it's Project DOI: 10.21228/M8159B This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST000001 |
| Study Title | Fatb Induction Experiment (FatBIE) |
| Study Type | Genotype treatment |
| Study Summary | This experiment tests the consequence of a mutation at the FatB gene (At1g08510) in the wound-response of Arabidopsis. The FatB mutant allele (fatb KD J. Ohlrogge (Plant Cell 2003, Vol 15, 1020-1033)) was obtained from Dr. Katayonn Dehesh, University of California, Davis, Davis, CA. This allele is in the Ws background.The standardized growth conditions are as follows: 1. Seeds (between 14 and 16) are sown on media in 100 x 100 x 15mm square Falcon Petri Dishes (Fisher Scientific, catalogue #08-757-11A). Seeds were arranged on the plates in a single horizontal line at the 1-cm mark from the top of the plate.2. Each plate contains between 20 and 25-ml of sterile MS media containing 0.1% (w/v) sucrose.3. Prior to sowing, seeds were sterilized by treating for 1 minute at room temperature with a 300-l solution of 50% (v/v) ethanol, this solution was removed and replaced with a 300-l solution consisting of 1% (v/v) Tween 20 (Fischer BioReagents, catalogue #BP33750), and 50% (v/v) bleach solution (Clorox), and incubated at room temperature for 10-minutes. The seeds were then washed with three changes of 0.3-ml of sterile water. |
| Institute | University of California, Davis |
| Department | Davis Genome Center |
| Laboratory | Fiehn |
| Last Name | Kind |
| First Name | Tobias |
| Address | 451 E. Health Sci. Drive, Davis, CA 95616, USA |
| tkind@ucdavis.edu | |
| Submit Date | 2013-01-15 |
| Num Groups | 4 |
| Total Subjects | 24 |
| Raw Data Available | No |
| Analysis Type Detail | GC-MS |
| Release Date | 2013-02-14 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Factors:
Subject type: Plant; Subject species: Arabidopsis thaliana (Factor headings shown in green)
| mb_sample_id | local_sample_id | Arabidopsis Genotype | Plant Wounding Treatment |
|---|---|---|---|
| SA000019 | LabF_115904 | fatb-ko KD; At1g08510 | Control - Non-Wounded |
| SA000020 | LabF_115909 | fatb-ko KD; At1g08510 | Control - Non-Wounded |
| SA000021 | LabF_115914 | fatb-ko KD; At1g08510 | Control - Non-Wounded |
| SA000022 | LabF_115919 | fatb-ko KD; At1g08510 | Control - Non-Wounded |
| SA000023 | LabF_115924 | fatb-ko KD; At1g08510 | Control - Non-Wounded |
| SA000024 | LabF_115929 | fatb-ko KD; At1g08510 | Control - Non-Wounded |
| SA000007 | LabF_115842 | fatb-ko KD; At1g08510 | Wounded |
| SA000008 | LabF_115847 | fatb-ko KD; At1g08510 | Wounded |
| SA000009 | LabF_115852 | fatb-ko KD; At1g08510 | Wounded |
| SA000010 | LabF_115857 | fatb-ko KD; At1g08510 | Wounded |
| SA000011 | LabF_115862 | fatb-ko KD; At1g08510 | Wounded |
| SA000012 | LabF_115867 | fatb-ko KD; At1g08510 | Wounded |
| SA000013 | LabF_115873 | Wassilewskija (Ws) | Control - Non-Wounded |
| SA000014 | LabF_115878 | Wassilewskija (Ws) | Control - Non-Wounded |
| SA000015 | LabF_115883 | Wassilewskija (Ws) | Control - Non-Wounded |
| SA000016 | LabF_115888 | Wassilewskija (Ws) | Control - Non-Wounded |
| SA000017 | LabF_115893 | Wassilewskija (Ws) | Control - Non-Wounded |
| SA000018 | LabF_115898 | Wassilewskija (Ws) | Control - Non-Wounded |
| SA000001 | LabF_115811 | Wassilewskija (Ws) | Wounded |
| SA000002 | LabF_115816 | Wassilewskija (Ws) | Wounded |
| SA000003 | LabF_115821 | Wassilewskija (Ws) | Wounded |
| SA000004 | LabF_115826 | Wassilewskija (Ws) | Wounded |
| SA000005 | LabF_115831 | Wassilewskija (Ws) | Wounded |
| SA000006 | LabF_115836 | Wassilewskija (Ws) | Wounded |
| Showing results 1 to 24 of 24 |
