Summary of study ST000001

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Study IDST000001
Study TitleFatb Induction Experiment (FatBIE)
Study TypeGenotype treatment
Study SummaryThis experiment tests the consequence of a mutation at the FatB gene (At1g08510) in the wound-response of Arabidopsis. The FatB mutant allele (fatb KD J. Ohlrogge (Plant Cell 2003, Vol 15, 1020-1033)) was obtained from Dr. Katayonn Dehesh, University of California, Davis, Davis, CA. This allele is in the Ws background.The standardized growth conditions are as follows: 1. Seeds (between 14 and 16) are sown on media in 100 x 100 x 15mm square Falcon Petri Dishes (Fisher Scientific, catalogue #08-757-11A). Seeds were arranged on the plates in a single horizontal line at the 1-cm mark from the top of the plate.2. Each plate contains between 20 and 25-ml of sterile MS media containing 0.1% (w/v) sucrose.3. Prior to sowing, seeds were sterilized by treating for 1 minute at room temperature with a 300-l solution of 50% (v/v) ethanol, this solution was removed and replaced with a 300-l solution consisting of 1% (v/v) Tween 20 (Fischer BioReagents, catalogue #BP33750), and 50% (v/v) bleach solution (Clorox), and incubated at room temperature for 10-minutes. The seeds were then washed with three changes of 0.3-ml of sterile water.
InstituteUniversity of California, Davis
DepartmentDavis Genome Center
Last NameKind
First NameTobias
Address451 E. Health Sci. Drive, Davis, CA 95616, USA
Submit Date2013-01-15
Num Groups4
Total Subjects24
Raw Data AvailableNo
Release Date2013-02-14
Release Version1

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Project ID:PR000001
Project DOI:doi: 10.21228/M8159B
Project Title:FatB Gene Project
Project Type:Genotype treatment
Project Summary:Experiment to test the consequence of a mutation at the FatB gene (At1g08510) in the wound-response of Arabidopsis
Institute:University of California, Davis
Department:Davis Genome Center
Last Name:Fiehn
First Name:Oliver
Address:451 E. Health Sci. Drive, Davis, CA, 95616, USA
Publications:Quality control for plant metabolomics: reporting MSI-compliant studies. DOI: 10.1111/j.1365-313X.2007.03387.x PubMed