Summary of Study ST000370

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000129. The data can be accessed directly via it's Project DOI: 10.21228/M85C7W This work is supported by NIH grant, U2C- DK119886.

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Study IDST000370
Study TitlePrimary T Cell Noxa Knockdown (Donor 8) III
Study TypeRegular
Study SummaryLabeling of cells was carried out in triplicate with each sample containing 35e6. Cells were deprived of glucose or glutamine for 1 h then resuspended in 10mM [13C1,2] D-glucose labeled or 4 mM [13C5]-U-glutamine labeled medium, respectively. At this time cells were also activated with 5 ug/ml anti-CD3 and anti-CD28. Cells were labeled (and activated) for 16 h when samples were spun down, and cells were resuspended in 200 ul ice cold methanol. For this part of the experiment, the spent medium was collected for analysis. All samples were immediately stored in -80oC.
Institute
University of Michigan
DepartmentBiomedical Research
LaboratoryCore Facilities Metabolomics core
Last NameKachman
First NameMaureen
Address6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714
Emailmkachman@med.umich.edu
Phone(734) 232-8175
Submit Date2015-03-27
Num Groups3
Total Subjects16
Raw Data AvailableYes
Raw Data File Type(s)d
Analysis Type DetailGC-MS
Release Date2016-12-22
Release Version1
Maureen Kachman Maureen Kachman
https://dx.doi.org/10.21228/M85C7W
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR000129
Project DOI:doi: 10.21228/M85C7W
Project Title:Metabolic effects of Noxa
Project Summary:labeled glutamine metabolomics on Noxa expressing cells
Institute:University of Minnesota
Department:Masonic Cancer Center
Laboratory:Kelekar Lab
Last Name:Kelekar
First Name:Ameeta
Address:420 Delaware Street SE, Minneapolis, MN 55455
Email:ameeta@umn.edu
Phone:612-626-2358

Subject:

Subject ID:SU000391
Subject Type:Animal
Subject Species:Homo sapiens
Taxonomy ID:9606
Species Group:Human

Factors:

Subject type: Animal; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id local_sample_id Glucose label Glutamine label
SA016894S00017978no no
SA016895S00017970no no
SA016896S00017971no yes
SA016897S00017964no yes
SA016898S00017969no yes
SA016899S00017965no yes
SA016900S00017966no yes
SA016901S00017967no yes
SA016902S00017968no yes
SA016903S00017979yes no
SA016904S00017977yes no
SA016905S00017972yes no
SA016906S00017973yes no
SA016907S00017974yes no
SA016908S00017975yes no
SA016909S00017976yes no
Showing results 1 to 16 of 16

Collection:

Collection ID:CO000385
Collection Summary:None
Sample Type:Culture Media

Treatment:

Treatment ID:TR000405
Treatment Summary:None

Sample Preparation:

Sampleprep ID:SP000398
Sampleprep Summary:None
Sampleprep Protocol Filename:Fluxomics analysis protocol-2015-03-11.docx

Combined analysis:

Analysis ID AN000604
Analysis type MS
Chromatography type GC
Chromatography system Agilent 7890A
Column Agilent DB5-MS (30m × 0.25mm, 0.25um)
MS Type EI
MS instrument type Single quadrupole
MS instrument name Agilent 5975C
Ion Mode POSITIVE
Units Area

Chromatography:

Chromatography ID:CH000430
Methods Filename:DQM0208
Instrument Name:Agilent 7890A
Column Name:Agilent DB5-MS (30m × 0.25mm, 0.25um)
Chromatography Type:GC

MS:

MS ID:MS000537
Analysis ID:AN000604
Instrument Name:Agilent 5975C
Instrument Type:Single quadrupole
MS Type:EI
Ion Mode:POSITIVE
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