Summary of Study ST000375

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000295. The data can be accessed directly via it's Project DOI: 10.21228/M8X60B This work is supported by NIH grant, U2C- DK119886.

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Study IDST000375
Study TitleMice exercise metabolomics (part II)
Study TypeRegular
Study SummaryMice were run for 15 minutes on a treadmill and then mice were induced into anesthesia at a dose of 5% isoflurane, then maintained by continuous inhalation of 2% isoflurane. Quadriceps muscle was collected first and flash frozen in liquid nitrogen. Then blood was collected from the left ventricle of the heart and placed into serum separating tubes. Blood was allowed to clot for 40 minutes and centrifuged, the supernatant was frozen in liquid nitrogen
Institute
University of Michigan
DepartmentBiomedical Research Core Facilities
LaboratoryMetabolomics core
Last NameKachman
First NameMaureen
Address6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714
Emailmkachman@med.umich.edu
Phone(734) 232-8175
Submit Date2016-04-21
Num Groups4
Total Subjects18
Raw Data AvailableYes
Raw Data File Type(s)d
Analysis Type DetailLC-MS
Release Date2016-12-22
Release Version1
Maureen Kachman Maureen Kachman
https://dx.doi.org/10.21228/M8X60B
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR000295
Project DOI:doi: 10.21228/M8X60B
Project Title:Metabolomics Mice Studies
Project Summary:Analysis of glycolysis/TCA cycle in skeletal muscle + acyl-carnitine in serum of wt and 113Q mice
Institute:University of Michigan
Department:Pathology
Laboratory:Lieberman, Andrew
Last Name:Lieberman
First Name:Andrew
Address:Ann Arbor, MI
Email:liebermn@umich.edu
Phone:734-936-1889

Subject:

Subject ID:SU000396
Subject Type:Animal
Subject Species:Mus musculus
Taxonomy ID:10090
Species Group:Mammal

Factors:

Subject type: Animal; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id local_sample_id Wild Type Mutants
SA017002S00018468no yes
SA017003S00018474no yes
SA017004S00018473no yes
SA017005S00018467no yes
SA017006S00018465no yes
SA017007S00018475no yes
SA017008S00018466no yes
SA017009S00018476no yes
SA017010S00018472yes no
SA017011S00018469yes no
SA017012S00018463yes no
SA017013S00018462yes no
SA017014S00018464yes no
SA017015S00018461yes no
SA017016S00018470yes no
SA017017S00018471yes no
Showing results 1 to 16 of 16

Collection:

Collection ID:CO000390
Collection Summary:NONE
Sample Type:Serum

Treatment:

Treatment ID:TR000410
Treatment Summary:None

Sample Preparation:

Sampleprep ID:SP000403
Sampleprep Summary:none
Sampleprep Protocol Filename:Gly-TCA-nucleotides analysis protocol-2015-03-09.docx

Combined analysis:

Analysis ID AN000609
Analysis type MS
Chromatography type HILIC
Chromatography system QTOF-002-HILIC-35min-1mm-No Insert.m.zip
Column Phenomenex Luna NH2 (150 x 1mm,3um)
MS Type ESI
MS instrument type Triple quadrupole
MS instrument name Agilent 6410A QQQ
Ion Mode NEGATIVE
Units pmol/mg tissue

Chromatography:

Chromatography ID:CH000435
Methods ID:DQM0166
Instrument Name:QTOF-002-HILIC-35min-1mm-No Insert.m.zip
Column Name:Phenomenex Luna NH2 (150 x 1mm,3um)
Chromatography Type:HILIC

MS:

MS ID:MS000542
Analysis ID:AN000609
Instrument Name:Agilent 6410A QQQ
Instrument Type:Triple quadrupole
MS Type:ESI
Ion Mode:NEGATIVE
Analysis Protocol File:QTOF-002-HILIC-35min-1mm-No_Insert.m.zip
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