Summary of Study ST000474

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000362. The data can be accessed directly via it's Project DOI: 10.21228/M8831S This work is supported by NIH grant, U2C- DK119886.

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Study IDST000474
Study TitleMetabolomic testing of a putative MDH inhibitor (targeted)
Study Typedrug treatment (single dose and timepoint)
Study SummaryHL-60 cells will be treated for 15hr with a putative MDH inhibitor or vehicle (n=5 per group)
Institute
University of Florida
DepartmentSECIM
LaboratoryCase Western University
Last NameWald
First NameDavid
Address2103 Cornell Rd WRB3-530 Cleveland, OH 44106
Emaildnw@case.edu
Phone2163685668
Submit Date2016-08-31
Num Groups2
Total Subjects20
Study CommentsTotal subjects: 10 for global testing and 10 for targeted testing
Raw Data File Type(s)raw(Thermo)
Analysis Type DetailLC-MS
Release Date2017-10-03
Release Version1
David Wald David Wald
https://dx.doi.org/10.21228/M8831S
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR000362
Project DOI:doi: 10.21228/M8831S
Project Title:Metabolomic testing of a putative MDH inhibitor
Project Summary:Testing for metabolomic changes after treatment of leukemia cells with a putative MDH inhibitor
Institute:Case Western Reserve University
Department:Pathology
Laboratory:Foundation and Institutional funds
Last Name:Wald
First Name:David
Address:2103 Cornell Rd WRB3-530 Cleveland, OH 44106
Email:dnw@case.edu
Phone:NA

Subject:

Subject ID:SU000495
Subject Type:Human leukemia cells
Subject Species:Homo sapiens
Taxonomy ID:9606
Cell Strain Details:HL-60
Subject Comments:immortalized
Cell Counts:10 million per sample
Species Group:Human

Factors:

Subject type: Human leukemia cells; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id local_sample_id Treatment
SA024328ut1-
SA024329ut5-
SA024330ut4-
SA024331ut2-
SA024332ut3-
SA024333tx5MDH inhib
SA024334tx4MDH inhib
SA024335tx1MDH inhib
SA024336tx2MDH inhib
SA024337tx3MDH inhib
Showing results 1 to 10 of 10

Collection:

Collection ID:CO000489
Collection Summary:Cells were centrifuged to remove growth media/drug and either flash frozen or washed 1x in PBS. The cells were washed for the targeted assay but not for the global assay.
Sample Type:cell
Storage Conditions:flash frozen
Tissue Cell Quantity Taken:10 million per sample

Treatment:

Treatment ID:TR000509
Treatment Summary:Cells were treated for 15hr with 1 micromolar of drug or vehicle
Cell Growth Container:flasks
Cell Growth Rate:~24hr per division
Cell Media:RPMI with 10% FBS
Cell Envir Cond:5% CO2 incubator
Cell Media Lastchanged:15 hr

Sample Preparation:

Sampleprep ID:SP000502
Sampleprep Summary:Amino Acid|Global
Sampleprep Protocol Filename:SB_OA_Assay.pdf
Appendix_C_Protein_DNA_quant.pdf
Sample Spiking:Appendix A - Internal Standard Prep GLCMS.pdf

Combined analysis:

Analysis ID AN000738
Analysis type MS
Chromatography type Reversed phase
Chromatography system Thermo Dionex Ultimate 3000
Column Waters Acquity BEH C18 (100 x 2mm,1.7um)
MS Type ESI
MS instrument type Triple quadrupole
MS instrument name Thermo Quantiva QQQ
Ion Mode POSITIVE
Units Total nmoles (with relative protein adjustment)

Chromatography:

Chromatography ID:CH000531
Chromatography Summary:Amino acids
Methods Filename:SB_OA_Assay.pdf
Instrument Name:Thermo Dionex Ultimate 3000
Column Name:Waters Acquity BEH C18 (100 x 2mm,1.7um)
Column Temperature:45
Flow Rate:0.3 mL/min
Solvent A:100% water; 0.1% formic acid
Solvent B:100% acetonitrile; 0.1% formic acid
Chromatography Type:Reversed phase

MS:

MS ID:MS000655
Analysis ID:AN000738
Instrument Name:Thermo Quantiva QQQ
Instrument Type:Triple quadrupole
MS Type:ESI
Ion Mode:POSITIVE
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