Summary of Study ST000729

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000526. The data can be accessed directly via it's Project DOI: 10.21228/M8P66F This work is supported by NIH grant, U2C- DK119886.

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Study IDST000729
Study TitleMetabolomics of rats after bariatric or sham surgery
Study TypeMS analysis
Study SummaryWe would like to examine the levels of amino acids in rats who have underwent bariatric (VSG, vertical sleeve gastrectomy) or sham (control) surgery. Plasma was collected after an overnight fast and once again after refeeding the rats for 2 hours.
Institute
University of Michigan
DepartmentBiomedical Research Core Facilities
LaboratoryMetabolomics core
Last NameKachman
First NameMaureen
Address6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714
Emailmkachman@med.umich.edu
Phone(734) 232-8175
Submit Date2017-06-19
Num Groups4
Total Subjects24
Raw Data AvailableYes
Raw Data File Type(s)d
Analysis Type DetailGC-MS
Release Date2017-12-06
Release Version1
Maureen Kachman Maureen Kachman
https://dx.doi.org/10.21228/M8P66F
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR000526
Project DOI:doi: 10.21228/M8P66F
Project Title:Ethicon Rat Study
Project Type:MS analysis
Project Summary:Amino acid analysis in plasma between Sham and VSG
Institute:University of Michigan
Department:Surgery
Laboratory:Seeley Lab
Last Name:Seeley
First Name:Randy
Address:Ann Arbor, MI
Email:seeleyrj@umich.edu
Phone:734-615-2880

Subject:

Subject ID:SU000752
Subject Type:Mouse
Subject Species:Mus musculus
Taxonomy ID:10090
Species Group:Mammal

Factors:

Subject type: Mouse; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id local_sample_id Diet Surgery
SA040884S00027588fasting overnight SHAM surgery
SA040885S00027590fasting overnight SHAM surgery
SA040886S00027594fasting overnight SHAM surgery
SA040887S00027586fasting overnight SHAM surgery
SA040888S00027592fasting overnight SHAM surgery
SA040889S00027584fasting overnight SHAM surgery
SA040890S00027578fasting overnight VSG surgery
SA040891S00027576fasting overnight VSG surgery
SA040892S00027572fasting overnight VSG surgery
SA040893S00027574fasting overnight VSG surgery
SA040894S00027580fasting overnight VSG surgery
SA040895S00027582fasting overnight VSG surgery
SA040896S00027591refed for 2 hours (45% HFD food) SHAM surgery
SA040897S00027593refed for 2 hours (45% HFD food) SHAM surgery
SA040898S00027589refed for 2 hours (45% HFD food) SHAM surgery
SA040899S00027595refed for 2 hours (45% HFD food) SHAM surgery
SA040900S00027587refed for 2 hours (45% HFD food) SHAM surgery
SA040901S00027585refed for 2 hours (45% HFD food) SHAM surgery
SA040902S00027581refed for 2 hours (45% HFD food) VSG surgery
SA040903S00027573refed for 2 hours (45% HFD food) VSG surgery
SA040904S00027583refed for 2 hours (45% HFD food) VSG surgery
SA040905S00027577refed for 2 hours (45% HFD food) VSG surgery
SA040906S00027575refed for 2 hours (45% HFD food) VSG surgery
SA040907S00027579refed for 2 hours (45% HFD food) VSG surgery
Showing results 1 to 24 of 24

Collection:

Collection ID:CO000746
Collection Summary:-
Sample Type:Blood

Treatment:

Treatment ID:TR000766
Treatment Summary:-

Sample Preparation:

Sampleprep ID:SP000759
Sampleprep Summary:-
Sampleprep Protocol Filename:A005 - Amino Acids.pdf

Combined analysis:

Analysis ID AN001142
Analysis type MS
Chromatography type GC
Chromatography system Agilent 7890N
Column Phenomenex Zebron ZB-AAA Amino Acid Analysis 10m x 0.25mm
MS Type EI
MS instrument type Single quadrupole
MS instrument name Agilent 5975
Ion Mode POSITIVE
Units uM

Chromatography:

Chromatography ID:CH000802
Instrument Name:Agilent 7890N
Column Name:Phenomenex Zebron ZB-AAA Amino Acid Analysis 10m x 0.25mm
Chromatography Type:GC

MS:

MS ID:MS001037
Analysis ID:AN001142
Instrument Name:Agilent 5975
Instrument Type:Single quadrupole
MS Type:EI
Ion Mode:POSITIVE
Analysis Protocol File:A005_Amino_Acids.pdf
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