#METABOLOMICS WORKBENCH westcoastmetabolomics_20231031_095502 DATATRACK_ID:4431 STUDY_ID:ST002952 ANALYSIS_ID:AN004848 PROJECT_ID:PR001835 VERSION 1 CREATED_ON October 31, 2023, 10:31 am #PROJECT PR:PROJECT_TITLE Investigate the impact of feeding time on the hexosamine biosynthetic pathway PR:PROJECT_TITLE (HBP) in the mouse liver and heart using targeted metabolomics: primary PR:PROJECT_TITLE metabolism PR:PROJECT_SUMMARY The overall goal of this project is to advance our understanding of PR:PROJECT_SUMMARY post-translational mechanisms that mediate metabolic regulation of PR:PROJECT_SUMMARY time-of-day-specific protein functions to orchestrate daily rhythms and maintain PR:PROJECT_SUMMARY homeostasis in animals. Robust daily biological rhythms over the 24-hour (h) PR:PROJECT_SUMMARY day-night cycles are key hallmarks of animal health span and are strongly PR:PROJECT_SUMMARY regulated by circadian clocks. Circadian clocks are cell autonomous molecular PR:PROJECT_SUMMARY timers present in the brain and in peripheral organs that enable animals to PR:PROJECT_SUMMARY adapt to predictable daily changes in environment and regulate rhythmic PR:PROJECT_SUMMARY processes such as sleep-wake cycles, feeding-fasting cycles, metabolism, PR:PROJECT_SUMMARY hormonal signaling and neuronal excitability. Besides light, the dominant time PR:PROJECT_SUMMARY cue for the brain clock, metabolic signals from clock-controlled feeding-fasting PR:PROJECT_SUMMARY cycles represent the most potent time cue to entrain and synchronize peripheral PR:PROJECT_SUMMARY clocks in key organs. Much effort has been dedicated to understanding the PR:PROJECT_SUMMARY metabolic regulation of daily biological rhythms, but many important mechanisms PR:PROJECT_SUMMARY are only just emerging. We recently established that metabolic signals from PR:PROJECT_SUMMARY feeding-fasting cycles regulate daily biological rhythms in Drosophila through PR:PROJECT_SUMMARY rhythmic O-linked-N-acetylglucosaminylation (O-GlcNAcylation). Protein PR:PROJECT_SUMMARY O-GlcNAcylation is a nutrient sensitive posttranslational modification (PTM) PR:PROJECT_SUMMARY that is tightly linked to metabolic status, as UDP-GlcNAc, the substrate of PR:PROJECT_SUMMARY O-GlcNAcylation, is produced from hexosamine biosynthetic pathway (HBP), which PR:PROJECT_SUMMARY integrates the metabolites from glucose, amino acid, lipid and nucleotide PR:PROJECT_SUMMARY metabolism. We now propose to investigate whether feeding activity can regulate PR:PROJECT_SUMMARY daily O-GlcNAcylation rhythm in mouse liver and heart and whether the levels of PR:PROJECT_SUMMARY HBP metabolites in mouse liver and heart are affected by different feeding time PR:PROJECT_SUMMARY within a day/night cycle. Here, we restricted the feeding time of C57BL/6 male PR:PROJECT_SUMMARY mice to ZT12-24 (RF12-24. ZT, zeitgeber time; ZT0 indicates light on, while ZT12 PR:PROJECT_SUMMARY indicates light off) v.s. ZT0-12 (RF0-12) for 3 weeks and collected liver and PR:PROJECT_SUMMARY heart tissues every 4 hours over a 24-hour period. The liver and heart samples PR:PROJECT_SUMMARY were subjected to targeted metabolomic analysis for HBP metabolites. PR:INSTITUTE University of California, Davis PR:DEPARTMENT Department of Entomology and Nematology PR:LABORATORY Chiu lab PR:LAST_NAME Chiu PR:FIRST_NAME Joanna PR:ADDRESS 6352 Storer Hall, One Shields Avenue, Davis, CA 95616, USA PR:EMAIL jcchiu@ucdavis.edu PR:PHONE (530) 752-1643 #STUDY ST:STUDY_TITLE Investigate the impact of feeding time on the hexosamine biosynthetic pathway ST:STUDY_TITLE (HBP) in the mouse liver and heart using targeted metabolomics: primary ST:STUDY_TITLE metabolism ST:STUDY_SUMMARY The overall goal of this project is to advance our understanding of ST:STUDY_SUMMARY post-translational mechanisms that mediate metabolic regulation of ST:STUDY_SUMMARY time-of-day-specific protein functions to orchestrate daily rhythms and maintain ST:STUDY_SUMMARY homeostasis in animals. Robust daily biological rhythms over the 24-hour (h) ST:STUDY_SUMMARY day-night cycles are key hallmarks of animal health span and are strongly ST:STUDY_SUMMARY regulated by circadian clocks. Circadian clocks are cell autonomous molecular ST:STUDY_SUMMARY timers present in the brain and in peripheral organs that enable animals to ST:STUDY_SUMMARY adapt to predictable daily changes in environment and regulate rhythmic ST:STUDY_SUMMARY processes such as sleep-wake cycles, feeding-fasting cycles, metabolism, ST:STUDY_SUMMARY hormonal signaling and neuronal excitability. Besides light, the dominant time ST:STUDY_SUMMARY cue for the brain clock, metabolic signals from clock-controlled feeding-fasting ST:STUDY_SUMMARY cycles represent the most potent time cue to entrain and synchronize peripheral ST:STUDY_SUMMARY clocks in key organs. Much effort has been dedicated to understanding the ST:STUDY_SUMMARY metabolic regulation of daily biological rhythms, but many important mechanisms ST:STUDY_SUMMARY are only just emerging. We recently established that metabolic signals from ST:STUDY_SUMMARY feeding-fasting cycles regulate daily biological rhythms in Drosophila through ST:STUDY_SUMMARY rhythmic O-linked-N-acetylglucosaminylation (O-GlcNAcylation). Protein ST:STUDY_SUMMARY O-GlcNAcylation is a nutrient sensitive posttranslational modification (PTM) ST:STUDY_SUMMARY that is tightly linked to metabolic status, as UDP-GlcNAc, the substrate of ST:STUDY_SUMMARY O-GlcNAcylation, is produced from hexosamine biosynthetic pathway (HBP), which ST:STUDY_SUMMARY integrates the metabolites from glucose, amino acid, lipid and nucleotide ST:STUDY_SUMMARY metabolism. We now propose to investigate whether feeding activity can regulate ST:STUDY_SUMMARY daily O-GlcNAcylation rhythm in mouse liver and heart and whether the levels of ST:STUDY_SUMMARY HBP metabolites in mouse liver and heart are affected by different feeding time ST:STUDY_SUMMARY within a day/night cycle. Here, we restricted the feeding time of C57BL/6 male ST:STUDY_SUMMARY mice to ZT12-24 (RF12-24. ZT, zeitgeber time; ZT0 indicates light on, while ZT12 ST:STUDY_SUMMARY indicates light off) v.s. ZT0-12 (RF0-12) for 3 weeks and collected liver and ST:STUDY_SUMMARY heart tissues every 4 hours over a 24-hour period. The liver and heart samples ST:STUDY_SUMMARY were subjected to targeted metabolomic analysis for HBP metabolites. ST:INSTITUTE University of California, Davis ST:DEPARTMENT Department of Entomology and Nematology ST:LABORATORY Chiu lab ST:LAST_NAME Chiu ST:FIRST_NAME Joanna ST:ADDRESS 6352 Storer Hall, One Shields Avenue, Davis, CA 95616, USA ST:EMAIL jcchiu@ucdavis.edu ST:PHONE (530) 752-1643 #SUBJECT SU:SUBJECT_TYPE Mammal SU:SUBJECT_SPECIES Mus musculus SU:TAXONOMY_ID 10090 #SUBJECT_SAMPLE_FACTORS: SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Raw file names and additional sample data SUBJECT_SAMPLE_FACTORS - Heart ZT11-rep1_043 organ:Heart | Treatment:Control RAW_FILE_NAME=211227aZTsa01_1 SUBJECT_SAMPLE_FACTORS - Heart ZT11-rep2_044 organ:Heart | Treatment:Control RAW_FILE_NAME=211227aZTsa02_1 SUBJECT_SAMPLE_FACTORS - Heart ZT11-rep3_045 organ:Heart | Treatment:Control RAW_FILE_NAME=211227aZTsa03_1 SUBJECT_SAMPLE_FACTORS - Heart ZT15-rep1_046 organ:Heart | Treatment:Control RAW_FILE_NAME=211227aZTsa04_1 SUBJECT_SAMPLE_FACTORS - Heart ZT15-rep2_047 organ:Heart | Treatment:Control RAW_FILE_NAME=211227aZTsa05_1 SUBJECT_SAMPLE_FACTORS - Heart ZT15-rep3_048 organ:Heart | Treatment:Control RAW_FILE_NAME=211227aZTsa06_1 SUBJECT_SAMPLE_FACTORS - Heart ZT19-rep1_049 organ:Heart | Treatment:Control RAW_FILE_NAME=211227aZTsa07_1 SUBJECT_SAMPLE_FACTORS - Heart ZT19-rep2_050 organ:Heart | Treatment:Control RAW_FILE_NAME=211227aZTsa08_1 SUBJECT_SAMPLE_FACTORS - Heart ZT19-rep3_051 organ:Heart | Treatment:Control RAW_FILE_NAME=211227aZTsa09_1 SUBJECT_SAMPLE_FACTORS - Heart ZT23-rep1_052 organ:Heart | Treatment:Control RAW_FILE_NAME=211227aZTsa10_1 SUBJECT_SAMPLE_FACTORS - Heart ZT23-rep2_053 organ:Heart | Treatment:Control RAW_FILE_NAME=211227aZTsa11_1 SUBJECT_SAMPLE_FACTORS - Heart ZT23-rep3_054 organ:Heart | Treatment:Control RAW_FILE_NAME=211227aZTsa12_1 SUBJECT_SAMPLE_FACTORS - Heart ZT3-rep1_037 organ:Heart | Treatment:Control RAW_FILE_NAME=211227aZTsa13_1 SUBJECT_SAMPLE_FACTORS - Heart ZT3-rep2_038 organ:Heart | Treatment:Control RAW_FILE_NAME=211227aZTsa14_1 SUBJECT_SAMPLE_FACTORS - Heart ZT3-rep3_039 organ:Heart | Treatment:Control RAW_FILE_NAME=211227aZTsa15_1 SUBJECT_SAMPLE_FACTORS - Heart ZT7-rep1_040 organ:Heart | Treatment:Control RAW_FILE_NAME=211227aZTsa16_1 SUBJECT_SAMPLE_FACTORS - Heart ZT7-rep2_041 organ:Heart | Treatment:Control RAW_FILE_NAME=211227aZTsa17_1 SUBJECT_SAMPLE_FACTORS - Heart ZT7-rep3_042 organ:Heart | Treatment:Control RAW_FILE_NAME=211227aZTsa18_1 SUBJECT_SAMPLE_FACTORS - Heart ZT11-rep1_061 organ:Heart | Treatment:Expt RAW_FILE_NAME=211227aZTsa19_1 SUBJECT_SAMPLE_FACTORS - Heart ZT11-rep2_062 organ:Heart | Treatment:Expt RAW_FILE_NAME=211227aZTsa20_1 SUBJECT_SAMPLE_FACTORS - Heart ZT11rep3_063 organ:Heart | Treatment:Expt RAW_FILE_NAME=211227aZTsa21_1 SUBJECT_SAMPLE_FACTORS - Heart ZT15-rep1_064 organ:Heart | Treatment:Expt RAW_FILE_NAME=211227aZTsa22_1 SUBJECT_SAMPLE_FACTORS - Heart ZT15-rep2_065 organ:Heart | Treatment:Expt RAW_FILE_NAME=211227aZTsa23_1 SUBJECT_SAMPLE_FACTORS - Heart ZT15-rep3_066 organ:Heart | Treatment:Expt RAW_FILE_NAME=211227aZTsa24_1 SUBJECT_SAMPLE_FACTORS - Heart ZT19-rep1_067 organ:Heart | Treatment:Expt RAW_FILE_NAME=211227aZTsa25_1 SUBJECT_SAMPLE_FACTORS - Heart ZT19-rep2_068 organ:Heart | Treatment:Expt RAW_FILE_NAME=211227aZTsa26_1 SUBJECT_SAMPLE_FACTORS - Heart ZT19-rep3_069 organ:Heart | Treatment:Expt RAW_FILE_NAME=211227aZTsa27_1 SUBJECT_SAMPLE_FACTORS - Heart ZT23-rep1_070 organ:Heart | Treatment:Expt RAW_FILE_NAME=211227aZTsa28_1 SUBJECT_SAMPLE_FACTORS - Heart ZT23-rep2_071 organ:Heart | Treatment:Expt RAW_FILE_NAME=211227aZTsa29_1 SUBJECT_SAMPLE_FACTORS - Heart ZT23-rep3_072 organ:Heart | Treatment:Expt RAW_FILE_NAME=211227aZTsa30_1 SUBJECT_SAMPLE_FACTORS - Heart ZT3-rep1_055 organ:Heart | Treatment:Expt RAW_FILE_NAME=211227aZTsa31_1 SUBJECT_SAMPLE_FACTORS - Heart ZT3-rep2_056 organ:Heart | Treatment:Expt RAW_FILE_NAME=211227aZTsa32_1 SUBJECT_SAMPLE_FACTORS - Heart ZT3-rep3_057 organ:Heart | Treatment:Expt RAW_FILE_NAME=211227aZTsa33_1 SUBJECT_SAMPLE_FACTORS - Heart ZT7-rep1_058 organ:Heart | Treatment:Expt RAW_FILE_NAME=211227aZTsa34_1 SUBJECT_SAMPLE_FACTORS - Heart ZT7-rep2_059 organ:Heart | Treatment:Expt RAW_FILE_NAME=211227aZTsa35_1 SUBJECT_SAMPLE_FACTORS - Heart ZT7-rep3_060 organ:Heart | Treatment:Expt RAW_FILE_NAME=211227aZTsa36_1 SUBJECT_SAMPLE_FACTORS - Liver ZT11-rep1_007 organ:Liver | Treatment:Control RAW_FILE_NAME=211227aZTsa37_1 SUBJECT_SAMPLE_FACTORS - Liver ZT11-rep2_008 organ:Liver | Treatment:Control RAW_FILE_NAME=211227aZTsa38_1 SUBJECT_SAMPLE_FACTORS - Liver ZT11-rep3_009 organ:Liver | Treatment:Control RAW_FILE_NAME=211227aZTsa39_1 SUBJECT_SAMPLE_FACTORS - Liver ZT15-rep1_010 organ:Liver | Treatment:Control RAW_FILE_NAME=211227aZTsa40_1 SUBJECT_SAMPLE_FACTORS - Liver ZT15-rep2_011 organ:Liver | Treatment:Control RAW_FILE_NAME=211227aZTsa41_1 SUBJECT_SAMPLE_FACTORS - Liver ZT15-rep3_012 organ:Liver | Treatment:Control RAW_FILE_NAME=211227aZTsa42_1 SUBJECT_SAMPLE_FACTORS - Liver ZT19-rep1_013 organ:Liver | Treatment:Control RAW_FILE_NAME=211227aZTsa43_1 SUBJECT_SAMPLE_FACTORS - Liver ZT19-rep2_014 organ:Liver | Treatment:Control RAW_FILE_NAME=211227aZTsa44_1 SUBJECT_SAMPLE_FACTORS - Liver ZT19-rep3_015 organ:Liver | Treatment:Control RAW_FILE_NAME=211227aZTsa45_1 SUBJECT_SAMPLE_FACTORS - Liver ZT23-rep1_016 organ:Liver | Treatment:Control RAW_FILE_NAME=211227aZTsa46_1 SUBJECT_SAMPLE_FACTORS - Liver ZT23-rep2_017 organ:Liver | Treatment:Control RAW_FILE_NAME=211227aZTsa47_1 SUBJECT_SAMPLE_FACTORS - Liver ZT23-rep3_018 organ:Liver | Treatment:Control RAW_FILE_NAME=211227aZTsa48_1 SUBJECT_SAMPLE_FACTORS - Liver ZT3-rep1_001 organ:Liver | Treatment:Control RAW_FILE_NAME=211227aZTsa49_1 SUBJECT_SAMPLE_FACTORS - Liver ZT3-rep2_002 organ:Liver | Treatment:Control RAW_FILE_NAME=211227aZTsa50_1 SUBJECT_SAMPLE_FACTORS - Liver ZT3-rep3_003 organ:Liver | Treatment:Control RAW_FILE_NAME=211228aZTsa01_1 SUBJECT_SAMPLE_FACTORS - Liver ZT7-rep1_004 organ:Liver | Treatment:Control RAW_FILE_NAME=211228aZTsa02_1 SUBJECT_SAMPLE_FACTORS - Liver ZT7-rep2_005 organ:Liver | Treatment:Control RAW_FILE_NAME=211228aZTsa03_1 SUBJECT_SAMPLE_FACTORS - Liver ZT7-rep3_006 organ:Liver | Treatment:Control RAW_FILE_NAME=211228aZTsa04_1 SUBJECT_SAMPLE_FACTORS - Liver ZT11-rep1_025 organ:Liver | Treatment:Expt RAW_FILE_NAME=211228aZTsa05_1 SUBJECT_SAMPLE_FACTORS - Liver ZT11-rep2_026 organ:Liver | Treatment:Expt RAW_FILE_NAME=211228aZTsa06_1 SUBJECT_SAMPLE_FACTORS - Liver ZT11-rep3_027 organ:Liver | Treatment:Expt RAW_FILE_NAME=211228aZTsa07_1 SUBJECT_SAMPLE_FACTORS - Liver ZT15-rep1_028 organ:Liver | Treatment:Expt RAW_FILE_NAME=211228aZTsa08_1 SUBJECT_SAMPLE_FACTORS - Liver ZT15-rep2_029 organ:Liver | Treatment:Expt RAW_FILE_NAME=211228aZTsa09_1 SUBJECT_SAMPLE_FACTORS - Liver ZT15-rep3_030 organ:Liver | Treatment:Expt RAW_FILE_NAME=211228aZTsa10_1 SUBJECT_SAMPLE_FACTORS - Liver ZT19-rep1_031 organ:Liver | Treatment:Expt RAW_FILE_NAME=211228aZTsa11_1 SUBJECT_SAMPLE_FACTORS - Liver ZT19-rep2_032 organ:Liver | Treatment:Expt RAW_FILE_NAME=211228aZTsa12_1 SUBJECT_SAMPLE_FACTORS - Liver ZT19-rep3_033 organ:Liver | Treatment:Expt RAW_FILE_NAME=211228aZTsa13_1 SUBJECT_SAMPLE_FACTORS - Liver ZT23-rep1_034 organ:Liver | Treatment:Expt RAW_FILE_NAME=211228aZTsa14_1 SUBJECT_SAMPLE_FACTORS - Liver ZT23-rep2_035 organ:Liver | Treatment:Expt RAW_FILE_NAME=211228aZTsa15_1 SUBJECT_SAMPLE_FACTORS - Liver ZT23-rep3_036 organ:Liver | Treatment:Expt RAW_FILE_NAME=211228aZTsa16_1 SUBJECT_SAMPLE_FACTORS - Liver ZT3-rep1_019 organ:Liver | Treatment:Expt RAW_FILE_NAME=211228aZTsa17_1 SUBJECT_SAMPLE_FACTORS - Liver ZT3-rep2_020 organ:Liver | Treatment:Expt RAW_FILE_NAME=211228aZTsa18_1 SUBJECT_SAMPLE_FACTORS - Liver ZT3-rep3_021 organ:Liver | Treatment:Expt RAW_FILE_NAME=211228aZTsa19_1 SUBJECT_SAMPLE_FACTORS - Liver ZT7-rep1_022 organ:Liver | Treatment:Expt RAW_FILE_NAME=211228aZTsa20_1 SUBJECT_SAMPLE_FACTORS - Liver ZT7-rep2_023 organ:Liver | Treatment:Expt RAW_FILE_NAME=211228aZTsa21_1 SUBJECT_SAMPLE_FACTORS - Liver ZT7-rep3_024 organ:Liver | Treatment:Expt RAW_FILE_NAME=211228aZTsa22_1 SUBJECT_SAMPLE_FACTORS - Pool_001-H organ:Heart | Treatment:Heart Pool QC RAW_FILE_NAME=211228aZTsa23_1 SUBJECT_SAMPLE_FACTORS - Pool_002-H organ:Heart | Treatment:Heart Pool QC RAW_FILE_NAME=211228aZTsa24_1 SUBJECT_SAMPLE_FACTORS - Pool_003-H organ:Heart | Treatment:Heart Pool QC RAW_FILE_NAME=211228aZTsa25_1 SUBJECT_SAMPLE_FACTORS - Pool_001-L organ:Liver | Treatment:Liver Pool QC RAW_FILE_NAME=211228aZTsa26_1 SUBJECT_SAMPLE_FACTORS - Pool_005-L organ:Liver | Treatment:Liver Pool QC RAW_FILE_NAME=211228aZTsa27_1 SUBJECT_SAMPLE_FACTORS - Pool_006-L organ:Liver | Treatment:Liver Pool QC RAW_FILE_NAME=211228aZTsa28_1 #COLLECTION CO:COLLECTION_SUMMARY Mice were anesthetized using isoflurane. The liver or heart are dissected out CO:COLLECTION_SUMMARY and rinsed with PBS. Then samples were flash frozen in liquid nitrogen and CO:COLLECTION_SUMMARY stored at -80C. Samples were weight on dry ice before sending out for CO:COLLECTION_SUMMARY metabolomics analysis. CO:SAMPLE_TYPE Heart and Liver #TREATMENT TR:TREATMENT_SUMMARY The mice were either fed at natural feeding time (during nighttime, Control TR:TREATMENT_SUMMARY group) or at unnatural feeding time (during daytime, Expt group). #SAMPLEPREP SP:SAMPLEPREP_SUMMARY SOP -GCMS-02252019 #CHROMATOGRAPHY CH:CHROMATOGRAPHY_TYPE GC CH:INSTRUMENT_NAME Agilent 7890 CH:COLUMN_NAME Restek Rtx-5Sil MS (30m x 0.25mm, 0.25um) CH:SOLVENT_A NA CH:SOLVENT_B NA CH:FLOW_GRADIENT NA CH:FLOW_RATE 1 mL/min CH:COLUMN_TEMPERATURE NA #ANALYSIS AN:ANALYSIS_TYPE MS #MS MS:INSTRUMENT_NAME Leco Pegasus IV TOF MS:INSTRUMENT_TYPE GC-TOF MS:MS_TYPE EI MS:ION_MODE POSITIVE MS:MS_COMMENTS Mass spectrometer settings: A Leco Pegasus IV time of flight mass spectrometer MS:MS_COMMENTS is controlled by the Leco ChromaTOF software vs. 2.32 (St. Joseph, MI). The MS:MS_COMMENTS transfer line temperature between gas chromatograph and mass spectrometer is set MS:MS_COMMENTS to 280°C. Electron impact ionization at 70V is employed with an ion source MS:MS_COMMENTS temperature of 250°C. Acquisition rate is 17 spectra/second, with a scan mass MS:MS_COMMENTS range of 85-500 Da. #MS_METABOLITE_DATA MS_METABOLITE_DATA:UNITS ng/mg MS_METABOLITE_DATA_START Samples Heart ZT11-rep1_043 Heart ZT11-rep2_044 Heart ZT11-rep3_045 Heart ZT15-rep1_046 Heart ZT15-rep2_047 Heart ZT15-rep3_048 Heart ZT19-rep1_049 Heart ZT19-rep2_050 Heart ZT19-rep3_051 Heart ZT23-rep1_052 Heart ZT23-rep2_053 Heart ZT23-rep3_054 Heart ZT3-rep1_037 Heart ZT3-rep2_038 Heart ZT3-rep3_039 Heart ZT7-rep1_040 Heart ZT7-rep2_041 Heart ZT7-rep3_042 Heart ZT11-rep1_061 Heart ZT11-rep2_062 Heart ZT11rep3_063 Heart ZT15-rep1_064 Heart ZT15-rep2_065 Heart ZT15-rep3_066 Heart ZT19-rep1_067 Heart ZT19-rep2_068 Heart ZT19-rep3_069 Heart ZT23-rep1_070 Heart ZT23-rep2_071 Heart ZT23-rep3_072 Heart ZT3-rep1_055 Heart ZT3-rep2_056 Heart ZT3-rep3_057 Heart ZT7-rep1_058 Heart ZT7-rep2_059 Heart ZT7-rep3_060 Liver ZT11-rep1_007 Liver ZT11-rep2_008 Liver ZT11-rep3_009 Liver ZT15-rep1_010 Liver ZT15-rep2_011 Liver ZT15-rep3_012 Liver ZT19-rep1_013 Liver ZT19-rep2_014 Liver ZT19-rep3_015 Liver ZT23-rep1_016 Liver ZT23-rep2_017 Liver ZT23-rep3_018 Liver ZT3-rep1_001 Liver ZT3-rep2_002 Liver ZT3-rep3_003 Liver ZT7-rep1_004 Liver ZT7-rep2_005 Liver ZT7-rep3_006 Liver ZT11-rep1_025 Liver ZT11-rep2_026 Liver ZT11-rep3_027 Liver ZT15-rep1_028 Liver ZT15-rep2_029 Liver ZT15-rep3_030 Liver ZT19-rep1_031 Liver ZT19-rep2_032 Liver ZT19-rep3_033 Liver ZT23-rep1_034 Liver ZT23-rep2_035 Liver ZT23-rep3_036 Liver ZT3-rep1_019 Liver ZT3-rep2_020 Liver ZT3-rep3_021 Liver ZT7-rep1_022 Liver ZT7-rep2_023 Liver ZT7-rep3_024 Pool_001-H Pool_002-H Pool_003-H Pool_001-L Pool_005-L Pool_006-L Factors organ:Heart | Treatment:Control organ:Heart | Treatment:Control organ:Heart | Treatment:Control organ:Heart | Treatment:Control organ:Heart | Treatment:Control organ:Heart | Treatment:Control organ:Heart | Treatment:Control organ:Heart | Treatment:Control organ:Heart | Treatment:Control organ:Heart | Treatment:Control organ:Heart | Treatment:Control organ:Heart | Treatment:Control organ:Heart | Treatment:Control organ:Heart | Treatment:Control organ:Heart | Treatment:Control organ:Heart | Treatment:Control organ:Heart | Treatment:Control organ:Heart | Treatment:Control organ:Heart | Treatment:Expt organ:Heart | Treatment:Expt organ:Heart | Treatment:Expt organ:Heart | Treatment:Expt organ:Heart | Treatment:Expt organ:Heart | Treatment:Expt organ:Heart | Treatment:Expt organ:Heart | Treatment:Expt organ:Heart | Treatment:Expt organ:Heart | Treatment:Expt organ:Heart | Treatment:Expt organ:Heart | Treatment:Expt organ:Heart | Treatment:Expt organ:Heart | Treatment:Expt organ:Heart | Treatment:Expt organ:Heart | Treatment:Expt organ:Heart | Treatment:Expt organ:Heart | Treatment:Expt organ:Liver | Treatment:Control organ:Liver | Treatment:Control organ:Liver | Treatment:Control organ:Liver | Treatment:Control organ:Liver | Treatment:Control organ:Liver | Treatment:Control organ:Liver | Treatment:Control organ:Liver | Treatment:Control organ:Liver | Treatment:Control organ:Liver | Treatment:Control organ:Liver | Treatment:Control organ:Liver | Treatment:Control organ:Liver | Treatment:Control organ:Liver | Treatment:Control organ:Liver | Treatment:Control organ:Liver | Treatment:Control organ:Liver | Treatment:Control organ:Liver | Treatment:Control organ:Liver | Treatment:Expt organ:Liver | Treatment:Expt organ:Liver | Treatment:Expt organ:Liver | Treatment:Expt organ:Liver | Treatment:Expt organ:Liver | Treatment:Expt organ:Liver | Treatment:Expt organ:Liver | Treatment:Expt organ:Liver | Treatment:Expt organ:Liver | Treatment:Expt organ:Liver | Treatment:Expt organ:Liver | Treatment:Expt organ:Liver | Treatment:Expt organ:Liver | Treatment:Expt organ:Liver | Treatment:Expt organ:Liver | Treatment:Expt organ:Liver | Treatment:Expt organ:Liver | Treatment:Expt organ:Heart | Treatment:Heart Pool QC organ:Heart | Treatment:Heart Pool QC organ:Heart | Treatment:Heart Pool QC organ:Liver | Treatment:Liver Pool QC organ:Liver | Treatment:Liver Pool QC organ:Liver | Treatment:Liver Pool QC fructose-6-phosphate 55.6 72.1 56.5 65.6 82.4 72.7 74.4 84.4 158.0 108.9 67.8 77.8 88.3 67.8 91.4 60.8 69.3 63.3 91.3 66.4 85.5 65.9 62.7 95.8 123.2 66.7 65.1 77.9 72.2 77.6 101.0 52.4 90.9 69.6 57.0 65.5 67.7 87.7 90.7 56.5 108.2 73.9 81.7 59.4 79.4 73.3 63.3 58.5 87.6 63.3 88.8 73.0 91.1 63.1 90.1 85.5 91.8 88.4 72.2 74.8 90.9 72.8 83.5 83.8 75.8 91.3 64.1 67.5 90.8 69.8 77.4 85.6 88.8 82.8 70.7 69.2 62.9 67.1 UDP-N-acetylglucosamine 92.2 97.1 111.6 86.2 144.9 82.0 105.3 62.6 138.2 67.2 87.8 122.9 104.2 104.7 90.8 1.2 83.1 76.6 80.5 48.8 83.6 114.4 129.8 133.9 163.5 107.9 156.0 107.8 117.4 111.5 101.8 101.9 97.2 80.3 72.2 10.1 493.5 760.7 590.0 309.6 726.0 732.7 575.9 578.7 547.3 552.8 101.7 350.0 383.4 257.3 289.4 611.8 673.2 484.2 753.1 661.2 822.7 636.7 525.5 725.3 498.0 534.1 718.7 620.8 459.0 822.9 631.8 617.7 326.2 411.5 460.6 624.7 168.6 190.7 180.3 739.0 348.5 377.8 glutamine 42.4 36.9 33.5 41.3 47.6 40.5 40.7 48.1 36.8 66.6 45.9 35.3 57.2 37.1 39.8 52.7 36.8 37.5 48.4 43.8 35.3 55.7 44.6 51.5 51.8 54.5 48.2 46.3 43.4 54.4 50.3 28.3 48.8 49.3 33.8 48.2 39.6 54.6 50.8 45.4 44.6 47.9 45.2 47.7 50.7 44.2 51.1 40.9 51.3 48.0 48.6 37.2 54.2 35.7 53.2 52.2 52.0 50.5 46.4 50.2 81.3 44.4 54.9 49.2 45.5 56.8 40.8 36.6 47.0 45.3 43.8 47.8 47.5 41.7 38.6 39.9 36.1 36.1 MS_METABOLITE_DATA_END #METABOLITES METABOLITES_START metabolite_name ret.index quant mz BB id mass spec PubChem KEGG InChI Key fructose-6-phosphate 804279 315 6431 85:175.0 86:55.0 87:366.0 88:157.0 89:2236.0 90:436.0 92:4.0 94:18.0 96:197.0 98:32.0 99:369.0 100:408.0 101:1519.0 102:775.0 103:257.0 104:1131.0 105:281.0 106:108.0 109:60.0 111:305.0 113:195.0 114:242.0 115:86.0 116:26.0 117:911.0 118:370.0 120:83.0 121:101.0 122:34.0 124:89.0 126:163.0 128:84.0 129:4295.0 130:1211.0 131:850.0 132:289.0 133:2271.0 134:314.0 135:693.0 136:141.0 137:22.0 138:85.0 140:99.0 142:343.0 143:668.0 144:148.0 145:50.0 146:225.0 148:1100.0 149:903.0 151:230.0 152:41.0 153:197.0 154:246.0 156:580.0 157:115.0 158:170.0 163:344.0 164:46.0 165:37.0 166:34.0 167:42.0 168:113.0 170:283.0 172:370.0 173:456.0 174:216.0 175:11.0 178:134.0 179:30.0 180:178.0 181:157.0 182:84.0 183:180.0 184:33.0 185:103.0 187:5.0 188:145.0 189:737.0 190:593.0 191:2947.0 192:766.0 193:392.0 194:110.0 195:428.0 196:117.0 197:148.0 198:35.0 200:84.0 202:25.0 203:806.0 205:4257.0 206:2153.0 207:624.0 208:355.0 209:245.0 210:69.0 211:1611.0 212:297.0 213:160.0 214:197.0 215:87.0 216:167.0 217:4001.0 218:2207.0 220:374.0 222:30.0 224:19.0 225:589.0 226:75.0 227:454.0 228:181.0 229:147.0 230:371.0 231:39.0 232:210.0 233:34.0 234:109.0 235:50.0 236:30.0 237:12.0 239:103.0 240:124.0 241:46.0 242:76.0 244:238.0 245:330.0 246:293.0 247:51.0 248:111.0 249:21.0 250:44.0 251:2.0 252:83.0 253:147.0 254:74.0 256:138.0 257:59.0 260:260.0 261:156.0 262:242.0 263:121.0 264:77.0 268:11.0 269:165.0 270:118.0 271:10.0 272:97.0 273:139.0 275:161.0 276:1.0 277:51.0 278:45.0 280:8.0 281:28.0 282:21.0 283:233.0 284:37.0 285:256.0 286:67.0 287:54.0 288:63.0 289:97.0 290:70.0 291:91.0 292:52.0 294:179.0 298:172.0 299:3779.0 300:1095.0 301:638.0 302:280.0 303:14.0 306:171.0 307:136.0 309:45.0 313:47.0 314:215.0 315:7616.0 316:2132.0 317:1310.0 318:171.0 319:231.0 321:85.0 323:1.0 324:3.0 325:129.0 327:56.0 328:184.0 329:74.0 330:95.0 332:18.0 333:74.0 334:10.0 335:24.0 336:36.0 340:13.0 341:318.0 342:244.0 343:43.0 344:83.0 346:52.0 348:67.0 351:56.0 353:45.0 354:54.0 355:48.0 356:76.0 357:819.0 358:379.0 359:180.0 360:130.0 369:143.0 370:26.0 371:46.0 372:42.0 373:178.0 374:135.0 375:45.0 376:83.0 377:17.0 378:15.0 379:89.0 381:7.0 383:69.0 384:12.0 388:85.0 389:112.0 390:69.0 392:24.0 393:36.0 395:17.0 396:40.0 398:23.0 401:1.0 402:71.0 403:238.0 404:122.0 405:63.0 407:64.0 409:34.0 411:2.0 412:26.0 413:8.0 418:23.0 420:27.0 425:27.0 426:66.0 428:14.0 429:42.0 431:16.0 432:30.0 433:51.0 434:35.0 441:8.0 445:3.0 448:1.0 449:72.0 450:94.0 451:73.0 453:4.0 455:6.0 456:23.0 457:10.0 458:54.0 459:632.0 460:322.0 461:275.0 462:147.0 463:21.0 466:29.0 467:17.0 468:27.0 476:12.0 477:11.0 478:1.0 479:30.0 481:8.0 483:30.0 484:14.0 487:2.0 488:27.0 489:21.0 490:14.0 491:35.0 492:1.0 493:13.0 494:4.0 495:10.0 440641 C05345 BGWGXPAPYGQALX-ARQDHWQXSA-N UDP-N-acetylglucosamine 627437 226 42230 85:845.0 87:452.0 89:2984.0 90:244.0 91:2420.0 92:340.0 93:2451.0 94:1045.0 96:781.0 97:30.0 98:359.0 99:1727.0 100:2281.0 101:2398.0 102:454.0 103:8088.0 104:552.0 105:1225.0 106:26.0 107:923.0 108:711.0 109:152.0 110:133.0 111:657.0 112:189.0 113:1290.0 114:724.0 115:1580.0 116:2173.0 117:7350.0 118:603.0 119:1034.0 120:3458.0 121:3047.0 122:548.0 124:126.0 125:99.0 126:135.0 128:1479.0 129:6014.0 130:1188.0 131:2247.0 132:413.0 133:5153.0 134:1091.0 135:533.0 136:410.0 137:70.0 138:145.0 139:44.0 140:184.0 141:136.0 142:557.0 143:997.0 144:723.0 145:24.0 147:13614.0 148:1062.0 149:2058.0 150:549.0 151:124.0 152:190.0 154:270.0 155:307.0 156:2774.0 157:1634.0 158:626.0 159:1886.0 160:949.0 161:129.0 162:17.0 163:1075.0 164:63.0 165:48.0 166:82.0 167:7.0 168:1023.0 169:366.0 170:1823.0 171:236.0 172:305.0 173:907.0 174:347.0 175:368.0 177:298.0 178:90.0 179:80.0 181:17.0 182:1006.0 183:335.0 184:890.0 185:280.0 186:142.0 187:98.0 188:146.0 189:3213.0 190:704.0 191:3291.0 192:480.0 193:592.0 194:145.0 195:138.0 196:410.0 197:249.0 198:1089.0 199:116.0 200:262.0 201:443.0 202:805.0 203:1869.0 204:6221.0 205:1649.0 206:553.0 207:323.0 208:28.0 210:263.0 211:571.0 212:100.0 213:35.0 214:1501.0 215:1253.0 216:292.0 217:10494.0 218:4325.0 219:1199.0 220:233.0 222:60.0 224:450.0 225:29.0 226:3121.0 227:469.0 228:109.0 229:291.0 230:682.0 231:2275.0 232:474.0 233:2373.0 234:387.0 235:139.0 236:34.0 237:14.0 238:14.0 239:163.0 240:123.0 241:141.0 242:43.0 243:272.0 244:186.0 245:73.0 246:286.0 247:100.0 248:8.0 255:13.0 256:97.0 257:100.0 258:129.0 259:91.0 262:7.0 265:272.0 266:40.0 270:67.0 271:2.0 272:352.0 273:84.0 274:123.0 275:213.0 276:35.0 280:49.0 284:198.0 285:51.0 286:93.0 287:106.0 288:12.0 290:26.0 291:56.0 296:17.0 298:2.0 300:323.0 301:147.0 303:25.0 305:72.0 306:38.0 308:18.0 314:605.0 315:173.0 316:552.0 317:201.0 318:872.0 319:660.0 320:203.0 321:77.0 322:32.0 325:13.0 326:19.0 329:124.0 330:49.0 331:10.0 340:21.0 341:7.0 342:32.0 356:30.0 358:28.0 362:10.0 385:15.0 388:7.0 394:19.0 404:45.0 411:7.0 419:50.0 457:3.0 460:4.0 497:9.0 445675 C00043 LFTYTUAZOPRMMI-CFRASDGPSA-N glutamine 600000 156 171972 86:1008.0 87:256.0 88:222.0 90:245.0 94:189.0 96:280.0 97:328.0 98:717.0 100:3469.0 101:366.0 102:313.0 104:214.0 107:149.0 112:1011.0 113:678.0 114:1746.0 116:2134.0 118:20.0 119:321.0 121:86.0 126:499.0 128:4093.0 131:1699.0 132:1288.0 133:2351.0 139:1107.0 140:1327.0 141:56.0 142:377.0 144:424.0 145:1566.0 147:10332.0 148:1224.0 149:833.0 150:22.0 155:12949.0 156:35859.0 157:2758.0 158:1324.0 167:43.0 172:401.0 173:435.0 174:89.0 175:16.0 181:39.0 203:1829.0 211:142.0 213:151.0 216:276.0 218:961.0 227:378.0 228:103.0 229:1088.0 230:761.0 231:289.0 232:448.0 237:47.0 240:101.0 241:69.0 244:228.0 245:3073.0 246:703.0 247:353.0 248:125.0 257:309.0 258:218.0 269:73.0 272:157.0 273:200.0 290:58.0 301:72.0 303:52.0 320:47.0 327:54.0 347:500.0 348:51.0 349:50.0 363:116.0 405:54.0 459:62.0 468:43.0 476:35.0 500:39.0 5961 C00064 ZDXPYRJPNDTMRX-VKHMYHEASA-N METABOLITES_END #END