{
"METABOLOMICS WORKBENCH":{"STUDY_ID":"ST003098","ANALYSIS_ID":"AN005069","VERSION":"1","CREATED_ON":"February 18, 2024, 6:04 pm"},

"PROJECT":{"PROJECT_TITLE":"Probiotic intervention of FMT mice","PROJECT_SUMMARY":"L. plantarum intervention of gestational fecal microbiome transplant germ-free mice","INSTITUTE":"Beijing Sanyuan Foods Co. Ltd","LAST_NAME":"Bin","FIRST_NAME":"Liu","ADDRESS":"8th yingchang street, yinghai distric","EMAIL":"liubin@sanyuan.com.cn","PHONE":"01056306594"},

"STUDY":{"STUDY_TITLE":"probiotic on gestational health of FMT GF mice","STUDY_SUMMARY":"probiotic L.plantarum intervention gestational on FMT GF mice","INSTITUTE":"Beijing Sanyuan Foods Co. Ltd","LAST_NAME":"Liu","FIRST_NAME":"Bin","ADDRESS":"8th yingchang street, yinghai distric","EMAIL":"liubin@sanyuan.com.cn","PHONE":"01056306594"},

"SUBJECT":{"SUBJECT_TYPE":"Mammal","SUBJECT_SPECIES":"Mus musculus","TAXONOMY_ID":"10090","GENDER":"Female"},
"SUBJECT_SAMPLE_FACTORS":[
{
"Subject ID":"-",
"Sample ID":"908MN",
"Factors":{"Sample source":"cecum content","Factor":"control"},
"Additional sample data":{"RAW_FILE_NAME":"Wangyaru-P220801102.mzML"}
},
{
"Subject ID":"-",
"Sample ID":"188MN",
"Factors":{"Sample source":"cecum content","Factor":"control"},
"Additional sample data":{"RAW_FILE_NAME":"Wangyaru-P220801103.mzML"}
},
{
"Subject ID":"-",
"Sample ID":"909MN",
"Factors":{"Sample source":"cecum content","Factor":"control"},
"Additional sample data":{"RAW_FILE_NAME":"Wangyaru-P220801104.mzML"}
},
{
"Subject ID":"-",
"Sample ID":"185MN",
"Factors":{"Sample source":"cecum content","Factor":"control"},
"Additional sample data":{"RAW_FILE_NAME":"Wangyaru-P220801105.mzML"}
},
{
"Subject ID":"-",
"Sample ID":"180MN",
"Factors":{"Sample source":"cecum content","Factor":"intervention"},
"Additional sample data":{"RAW_FILE_NAME":"Wangyaru-P220801106.mzML"}
},
{
"Subject ID":"-",
"Sample ID":"907MN",
"Factors":{"Sample source":"cecum content","Factor":"intervention"},
"Additional sample data":{"RAW_FILE_NAME":"Wangyaru-P220801107.mzML"}
},
{
"Subject ID":"-",
"Sample ID":"81MN",
"Factors":{"Sample source":"cecum content","Factor":"intervention"},
"Additional sample data":{"RAW_FILE_NAME":"Wangyaru-P220801108.mzML"}
},
{
"Subject ID":"-",
"Sample ID":"107MN",
"Factors":{"Sample source":"cecum content","Factor":"intervention"},
"Additional sample data":{"RAW_FILE_NAME":"Wangyaru-P220801109.mzML"}
},
{
"Subject ID":"-",
"Sample ID":"120MN",
"Factors":{"Sample source":"cecum content","Factor":"intervention"},
"Additional sample data":{"RAW_FILE_NAME":"Wangyaru-P220801110.mzML"}
},
{
"Subject ID":"-",
"Sample ID":"122MN",
"Factors":{"Sample source":"cecum content","Factor":"intervention"},
"Additional sample data":{"RAW_FILE_NAME":"Wangyaru-P220801111.mzML"}
}
],
"COLLECTION":{"COLLECTION_SUMMARY":"cecum content sample was collected and stored at -80°C environment","SAMPLE_TYPE":"Cecum","STORAGE_CONDITIONS":"-80℃"},

"TREATMENT":{"TREATMENT_SUMMARY":"mice were randomly assigned into two experimental groups, named control and intervention groups, receiving standard chow feeding, and standard chow + L. plantarum"},

"SAMPLEPREP":{"SAMPLEPREP_SUMMARY":"sample was extracted in 100 μL of 80% methanol, and were centrifuged at 4 °C for 10 min at 12000 rpm after vortexing for 120s and the supernatant was resolved in water and filtered and then transferred into the vial","PROCESSING_STORAGE_CONDITIONS":"4℃","EXTRACT_STORAGE":"-80℃"},

"CHROMATOGRAPHY":{"CHROMATOGRAPHY_TYPE":"LC","INSTRUMENT_NAME":"Waters Acquity","COLUMN_NAME":"Waters ACQUITY UPLC HSS T3 (150 x 2.1mm,1.8um)","SOLVENT_A":"formic acid in water","SOLVENT_B":"formic acid in methanol","FLOW_GRADIENT":"1% B； 2~3 min， 1~30% B； 3~3.5 min， 30% B； 4.5~8 min， 30~50% B； 8~10 min， 50~95% B； 10~11 min， 95% B； 11~17 min， 95~1% B","FLOW_RATE":"0.3 mL/min","COLUMN_TEMPERATURE":"40"},

"ANALYSIS":{"ANALYSIS_TYPE":"MS"},

"MS":{"INSTRUMENT_NAME":"ABI Sciex 5500 QTrap","INSTRUMENT_TYPE":"Triple quadrupole","MS_TYPE":"ESI","ION_MODE":"POSITIVE","MS_COMMENTS":"45 arbitrary units (AU), 13 AU, 1 AU, 350 °C, and 350 °C for sheath gas, aux gas, sweep gas, ion transfer tube, and vaporizer temperature, respectively. The ion source was operated using heated ESI with an ion spray voltage set at 5500 V in positive ion mode. Polarity switching and scheduled selected reaction monitoring (SRM) were employed.MS Convert software (Proteowizard, v3.0.8789) was applied to convert “.raw” format raw data to “.mzML” format, which would be used for downstream analysis."},

"MS_METABOLITE_DATA":{
"Units":"μg/g",

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etabolite":"Kyn","908MN":"0.241157864","188MN":"0.173010022","909MN":"0.211333202","185MN":"0.219403279","180MN":"0.1820084","907MN":"0.131200397","81MN":"0.139488048","107MN":"0.125891765","120MN":"0.080357931","122MN":"0.215306"},{"Metabolite":"MT","908MN":"","188MN":"","909MN":"","185MN":"","180MN":"","907MN":"","81MN":"","107MN":"","120MN":"","122MN":""},{"Metabolite":"NAS","908MN":"0.001702516","188MN":"0.002209974","909MN":"0.001402518","185MN":"0.001276403","180MN":"0.002199432","907MN":"0.001448036","81MN":"0.001481988","107MN":"0.001609047","120MN":"0.001449545","122MN":"0.00127028"},{"Metabolite":"5HTP","908MN":"0.039747359","188MN":"0.027684532","909MN":"0.040200791","185MN":"0.046100609","180MN":"","907MN":"0.025090238","81MN":"0.020358884","107MN":"0.026324824","120MN":"0.015847172","122MN":"0.02375952"},{"Metabolite":"HK","908MN":"","188MN":"","909MN":"","185MN":"","180MN":"","907MN":"","81MN":"","107MN":"","120MN":"","122MN":""},{"Metabolite":"MEL","908MN":"","188MN":"","909MN":"","185MN":"","180MN":"","907MN":"","81MN":"","107MN":"","120MN":"","122MN":""}],

"Metabolites":[{"Metabolite":"2AP","retention index":"1.95","quantified m/z":"110.053","PubChem ID":"0","KEGG ID":"0"},{"Metabolite":"IND","retention index":"9.29","quantified m/z":"118.273","PubChem ID":"0","KEGG ID":"0"},{"Metabolite":"Nam","retention index":"1.86","quantified m/z":"123.057","PubChem ID":"0","KEGG ID":"0"},{"Metabolite":"Na","retention index":"1.82","quantified m/z":"124.021","PubChem ID":"0","KEGG ID":"0"},{"Metabolite":"PA","retention index":"1.56","quantified m/z":"124.091","PubChem ID":"0","KEGG ID":"0"},{"Metabolite":"3MI","retention index":"10.8","quantified m/z":"132.071","PubChem ID":"0","KEGG ID":"0"},{"Metabolite":"IAld","retention index":"9.26","quantified m/z":"146.12","PubChem ID":"0","KEGG ID":"0"},{"Metabolite":"5-HAA","retention index":"1.72","quantified m/z":"154.082","PubChem ID":"0","KEGG ID":"0"},{"Metabolite":"3-HAA","retention index":"5.4","quantified m/z":"154.09","PubChem ID":"0","KEGG ID":"0"},{"Metabolite":"TRM","retention index":"5.87","quantified m/z":"161.053","PubChem ID":"0","KEGG ID":"0"},{"Metabolite":"IEt","retention index":"9.62","quantified m/z":"162.057","PubChem ID":"0","KEGG ID":"0"},{"Metabolite":"I3CA","retention index":"9.29","quantified m/z":"162.069","PubChem ID":"0","KEGG ID":"0"},{"Metabolite":"QA","retention index":"1.83","quantified m/z":"168.046","PubChem ID":"0","KEGG ID":"0"},{"Metabolite":"IAM","retention index":"7.62","quantified m/z":"175.054","PubChem ID":"0","KEGG ID":"0"},{"Metabolite":"IAA","retention index":"9.66","quantified m/z":"176.087","PubChem ID":"0","KEGG ID":"0"},{"Metabolite":"Ser","retention index":"2.83","quantified m/z":"177.122","PubChem ID":"0","KEGG ID":"0"},{"Metabolite":"IArA","retention index":"10.2","quantified m/z":"188.023","PubChem ID":"0","KEGG ID":"0"},{"Metabolite":"IPA","retention index":"10.2","quantified m/z":"189.993","PubChem ID":"0","KEGG ID":"0"},{"Metabolite":"KynA","retention index":"6.78","quantified m/z":"190.031","PubChem ID":"0","KEGG ID":"0"},{"Metabolite":"MeOTA","retention index":"6.11","quantified m/z":"191.252","PubChem ID":"0","KEGG ID":"0"},{"Metabolite":"5-HIAA","retention index":"6.22","quantified m/z":"192.048","PubChem ID":"0","KEGG ID":"0"},{"Metabolite":"Trp","retention index":"5.86","quantified m/z":"205.037","PubChem ID":"0","KEGG ID":"0"},{"Metabolite":"XA","retention index":"6.48","quantified m/z":"206.054","PubChem ID":"0","KEGG ID":"0"},{"Metabolite":"ILA","retention index":"8.95","quantified m/z":"206.081","PubChem ID":"0","KEGG ID":"0"},{"Metabolite":"Kyn","retention index":"3.63","quantified m/z":"209.089","PubChem ID":"0","KEGG ID":"0"},{"Metabolite":"MT","retention index":"6.9","quantified m/z":"219.102","PubChem ID":"0","KEGG ID":"0"},{"Metabolite":"NAS","retention index":"6.41","quantified m/z":"219.108","PubChem ID":"0","KEGG ID":"0"},{"Metabolite":"5HTP","retention index":"3.19","quantified m/z":"221.106","PubChem ID":"0","KEGG ID":"0"},{"Metabolite":"HK","retention index":"2.05","quantified m/z":"225.088","PubChem ID":"0","KEGG ID":"0"},{"Metabolite":"MEL","retention index":"9.33","quantified m/z":"233.159","PubChem ID":"0","KEGG ID":"0"}]
}

}