{
"METABOLOMICS WORKBENCH":{"STUDY_ID":"ST000370","ANALYSIS_ID":"AN000604","VERSION":"1","CREATED_ON":"April 18, 2016, 4:03 pm"},

"PROJECT":{"PROJECT_TITLE":"Metabolic effects of Noxa","PROJECT_SUMMARY":"labeled glutamine metabolomics on Noxa expressing cells","INSTITUTE":"Masonic Cancer Center, University of Minnesota","DEPARTMENT":"Masonic Cancer Center","LABORATORY":"Kelekar Lab","LAST_NAME":"Kelekar","FIRST_NAME":"Ameeta","ADDRESS":"420 Delaware Street SE, Minneapolis, MN 55455","EMAIL":"ameeta@umn.edu","PHONE":"612-626-2358"},

"STUDY":{"STUDY_TITLE":"Primary T Cell Noxa Knockdown (Donor 8) III","STUDY_TYPE":"Regular","STUDY_SUMMARY":"Labeling of cells was carried out in triplicate with each sample containing 35e6. Cells were deprived of glucose or glutamine for 1 h then resuspended in 10mM [13C1,2] D-glucose labeled or 4 mM [13C5]-U-glutamine labeled medium, respectively. At this time cells were also activated with 5 ug/ml anti-CD3 and anti-CD28. Cells were labeled (and activated) for 16 h when samples were spun down, and cells were resuspended in 200 ul ice cold methanol. For this part of the experiment, the spent medium was collected for analysis. All samples were immediately stored in -80oC.","INSTITUTE":"University of Michigan","DEPARTMENT":"Biomedical Research","LABORATORY":"Core Facilities Metabolomics core","LAST_NAME":"Kachman","FIRST_NAME":"Maureen","ADDRESS":"6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714","EMAIL":"mkachman@med.umich.edu","PHONE":"(734) 232-8175","NUM_GROUPS":"3","TOTAL_SUBJECTS":"16"},

"SUBJECT":{"SUBJECT_TYPE":"Animal","SUBJECT_SPECIES":"Homo sapiens","TAXONOMY_ID":"9606"},
"SUBJECT_SAMPLE_FACTORS":[
{
"Subject ID":"SU0012050",
"Sample ID":"S00017964",
"Factors":{"Glucose label":"no","Glutamine label":"yes"}
},
{
"Subject ID":"SU0012051",
"Sample ID":"S00017965",
"Factors":{"Glucose label":"no","Glutamine label":"yes"}
},
{
"Subject ID":"SU0012052",
"Sample ID":"S00017966",
"Factors":{"Glucose label":"no","Glutamine label":"yes"}
},
{
"Subject ID":"SU0012053",
"Sample ID":"S00017967",
"Factors":{"Glucose label":"no","Glutamine label":"yes"}
},
{
"Subject ID":"SU0012054",
"Sample ID":"S00017968",
"Factors":{"Glucose label":"no","Glutamine label":"yes"}
},
{
"Subject ID":"SU0012055",
"Sample ID":"S00017969",
"Factors":{"Glucose label":"no","Glutamine label":"yes"}
},
{
"Subject ID":"SU0012056",
"Sample ID":"S00017970",
"Factors":{"Glucose label":"no","Glutamine label":"no"}
},
{
"Subject ID":"SU0012057",
"Sample ID":"S00017971",
"Factors":{"Glucose label":"no","Glutamine label":"yes"}
},
{
"Subject ID":"SU0012058",
"Sample ID":"S00017972",
"Factors":{"Glucose label":"yes","Glutamine label":"no"}
},
{
"Subject ID":"SU0012059",
"Sample ID":"S00017973",
"Factors":{"Glucose label":"yes","Glutamine label":"no"}
},
{
"Subject ID":"SU0012060",
"Sample ID":"S00017974",
"Factors":{"Glucose label":"yes","Glutamine label":"no"}
},
{
"Subject ID":"SU0012061",
"Sample ID":"S00017975",
"Factors":{"Glucose label":"yes","Glutamine label":"no"}
},
{
"Subject ID":"SU0012062",
"Sample ID":"S00017976",
"Factors":{"Glucose label":"yes","Glutamine label":"no"}
},
{
"Subject ID":"SU0012063",
"Sample ID":"S00017977",
"Factors":{"Glucose label":"yes","Glutamine label":"no"}
},
{
"Subject ID":"SU0012056",
"Sample ID":"S00017978",
"Factors":{"Glucose label":"no","Glutamine label":"no"}
},
{
"Subject ID":"SU0012064",
"Sample ID":"S00017979",
"Factors":{"Glucose label":"yes","Glutamine label":"no"}
}
],
"COLLECTION":{"COLLECTION_SUMMARY":"None","SAMPLE_TYPE":"Culture Media"},

"TREATMENT":{"TREATMENT_SUMMARY":"None"},

"SAMPLEPREP":{"SAMPLEPREP_SUMMARY":"None","SAMPLEPREP_PROTOCOL_FILENAME":"Fluxomics analysis protocol-2015-03-11.docx"},

"CHROMATOGRAPHY":{"CHROMATOGRAPHY_TYPE":"GC","INSTRUMENT_NAME":"Agilent 7890A","COLUMN_NAME":"Agilent DB5-MS (30m × 0.25mm, 0.25um)","METHODS_FILENAME":"DQM0208"},

"ANALYSIS":{"ANALYSIS_TYPE":"MS"},

"MS":{"MS_COMMENTS":"-","INSTRUMENT_NAME":"Agilent 5975C","INSTRUMENT_TYPE":"Single quadrupole","MS_TYPE":"EI","ION_MODE":"POSITIVE"},

"MS_METABOLITE_DATA":{
"Units":"Area",

"Data":[{"Metabolite":"Aspartate _1234","S00017964":"9315.2","S00017965":"39815.0","S00017966":"16855.3","S00017967":"105275.1","S00017968":"108065.6","S00017969":"101531.6","S00017970":"95095.1","S00017971":"109268.8","S00017972":"80174.0","S00017973":"95234.6","S00017974":"105221.5","S00017975":"58153.4","S00017976":"40300.6","S00017977":"88247.7","S00017978":"98286.0","S00017979":"95446.6"},{"Metabolite":"Aspartate _1234_13C_1","S00017964":"163.7","S00017965":"414.8","S00017966":"741.0","S00017967":"129.6","S00017968":"","S00017969":"613.4","S00017970":"","S00017971":"","S00017972":"1322.4","S00017973":"","S00017974":"1179.7","S00017975":"6650.4","S00017976":"2777.2","S00017977":"534.5","S00017978":"1203.7","S00017979":"159.1"},{"Metabolite":"Aspartate _1234_13C_2","S00017964":"598.0","S00017965":"97.3","S00017966":"604.7","S00017967":"96.0","S00017968":"510.7","S00017969":"254.7","S00017970":"609.1","S00017971":"2.0","S00017972":"","S00017973":"239.8","S00017974":"340.5","S00017975":"","S00017976":"","S00017977":"1195.3","S00017978":"","S00017979":""},{"Metabolite":"Aspartate _1234_13C_3","S00017964":"648.6","S00017965":"876.1","S00017966":"444.4","S00017967":"159.4","S00017968":"","S00017969":"176.6","S00017970":"","S00017971":"882.1","S00017972":"","S00017973":"149.2","S00017974":"479.4","S00017975":"","S00017976":"","S00017977":"","S00017978":"","S00017979":"41.6"},{"Metabolite":"Aspartate _1234_13C_4","S00017964":"2967.2","S00017965":"2056.7","S00017966":"2788.6","S00017967":"131.6","S00017968":"83.9","S00017969":"43.0","S00017970":"","S00017971":"","S00017972":"","S00017973":"22.3","S00017974":"","S00017975":"","S00017976":"","S00017977":"","S00017978":"147.8","S00017979":"86.6"},{"Metabolite":"Lactate_123","S00017964":"6361118.7","S00017965":"4135958.7","S00017966":"5921024.1","S00017967":"161314.4","S00017968":"206333.0","S00017969":"185722.2","S00017970":"14659.9","S00017971":"18532.9","S00017972":"650301.8","S00017973":"519693.9","S00017974":"500979.3","S00017975":"710505.9","S00017976":"813099.8","S00017977":"468961.2","S00017978":"16255.5","S00017979":"10116.7"},{"Metabolite":"Lactate_123_13C_1","S00017964":"9254.1","S00017965":"17118.2","S00017966":"27882.9","S00017967":"441.6","S00017968":"347.3","S00017969":"423.4","S00017970":"","S00017971":"45.0","S00017972":"30369.6","S00017973":"27200.4","S00017974":"26542.4","S00017975":"31965.7","S00017976":"44127.8","S00017977":"22342.2","S00017978":"204.7","S00017979":"24.1"},{"Metabolite":"Lactate_123_13C_2","S00017964":"9366.8","S00017965":"2430.4","S00017966":"15325.5","S00017967":"1054.8","S00017968":"818.1","S00017969":"1037.7","S00017970":"","S00017971":"","S00017972":"558072.0","S00017973":"457561.6","S00017974":"446911.1","S00017975":"608078.6","S00017976":"680792.8","S00017977":"411714.2","S00017978":"2421.2","S00017979":"568.8"},{"Metabolite":"Lactate_123_13C_3","S00017964":"53806.2","S00017965":"27858.7","S00017966":"37259.5","S00017967":"421.6","S00017968":"342.0","S00017969":"431.2","S00017970":"284.7","S00017971":"","S00017972":"11642.4","S00017973":"8484.6","S00017974":"4941.8","S00017975":"9377.0","S00017976":"","S00017977":"7711.2","S00017978":"462.5","S00017979":""}],

"Metabolites":[{"Metabolite":"Lactate_123","retention index":"","quantified m/z":"","PubChem ID":"612","KEGG ID":""},{"Metabolite":"Lactate_123_13C_1","retention index":"","quantified m/z":"","PubChem ID":"612","KEGG ID":""},{"Metabolite":"Lactate_123_13C_2","retention index":"","quantified m/z":"","PubChem ID":"612","KEGG ID":""},{"Metabolite":"Lactate_123_13C_3","retention index":"","quantified m/z":"","PubChem ID":"612","KEGG ID":""},{"Metabolite":"Aspartate _1234","retention index":"","quantified m/z":"","PubChem ID":"424","KEGG ID":""},{"Metabolite":"Aspartate _1234_13C_1","retention index":"","quantified m/z":"","PubChem ID":"424","KEGG ID":""},{"Metabolite":"Aspartate _1234_13C_2","retention index":"","quantified m/z":"","PubChem ID":"424","KEGG ID":""},{"Metabolite":"Aspartate _1234_13C_3","retention index":"","quantified m/z":"","PubChem ID":"424","KEGG ID":""},{"Metabolite":"Aspartate _1234_13C_4","retention index":"","quantified m/z":"","PubChem ID":"424","KEGG ID":""}]
}

}