{
"METABOLOMICS WORKBENCH":{"STUDY_ID":"ST002989","ANALYSIS_ID":"AN004909","VERSION":"1","CREATED_ON":"November 29, 2023, 8:47 am"},

"PROJECT":{"PROJECT_TITLE":"Metabolic profiling of NPM1-deficient reparative BMDMs","PROJECT_SUMMARY":"BMDMs (1×107) were either induced toward the reparative phenotype as described above or left unstimulated. These BMDMs were then used to perform a metabolomics assay at Shanghai Biotree Biotech Co. Ltd. (China). Briefly, for each sample, 500 μl precooled MeOH/H2O (3/1, v/v) was added in. The samples were then vortexed for 30 sec. After precooling in dry ice, the samples were frozen and thawed three times in liquid nitrogen. They were then vortexed for 30 sec and sonicated for 15 min in an ice-water bath. Next, as a subsequent step, the samples were incubated at -40 °C for 1 h and centrifuged at 12000 rpm for 15 min at 4 °C (RCF = 13800 g, R = 8.6 cm). Afterwards, an aliquot of 400 μl of clear supernatant was collected and dried by spinning. As a reconstitution solution, mix 200 ml of ultrapure water with the residue. The reconstituted samples were vortexed before passing through the filter of the centrifuge tube, after which they were transferred to inserts in injection vials for HPLC-MS/MS analysis. The HPIC separation was carried out using an Thermo Scientific Dionex ICS-6000 HPIC System (Thermo Scientific), equipped with Dionex IonPac AS11-HC (2× 250 mm) and AG11-HC (2 mm×50 mm) columns. An AB SCIEX 6500 QTRAP+ triple quadrupole mass spectrometer (AB Sciex), equipped with an electrospray ionization (ESI) interface, in multiple reaction monitoring (MRM) mode, was applied for assay development. AB SCIEX Analyst Work Station Software (1.6.3 AB SCIEX), MultiQuant 3.0.3. software and Chromeleon7 were employed for MRM data acquisition and processing. The finalized dataset, inclusive of compound name, sample name, and concentration, was imported into SIMCA 16.0.2 software (Sartorius Stedim Data Analytics AB, Umea, Sweden) for comprehensive multivariate analysis. The data were subjected to scaling and logarithmic transformation to mitigate the effects of noise and excessive variance of the variables. After that, we utilized the R packages tidyverse, dplyr, magrittr, ggplot2, and ggrepel for data analysis and the construction of a volcano plot. Additionally, the R packages circlize and reshape2 were employed in the creation of a chord diagram. For the generation of a heatmap, we made use of the R package pheatmap.","INSTITUTE":"Renji Hospital, Shanghai Jiao Tong University School of Medicine","LAST_NAME":"Zhan","FIRST_NAME":"Zhenzhen","ADDRESS":"160 Pujian Road, Shanghai, Shanghai, 200127, China","EMAIL":"zhanzz2022@sjtu.edu.cn","PHONE":"86-21-61569249"},

"STUDY":{"STUDY_TITLE":"Targeting NPM1 Epigenetically Promotes Post-infarction Cardiac Repair by Reprogramming Reparative Macrophage Metabolism","STUDY_SUMMARY":"Reparative macrophages play a crucial role in limiting excessive fibrosis and promoting cardiac repair after myocardial infarction (MI), highlighting the significance of enhancing their reparative phenotype for wound healing. Metabolic adaptation orchestrates the phenotypic transition of macrophages; however, the precise mechanisms governing metabolic reprogramming of cardiac reparative macrophages remain poorly understood. In this study, we investigated the role of Nucleophosmin 1 (NPM1) in the metabolic and phenotypic shift of cardiac macrophages in the context of MI, and explored the effect of targeting NPM1 for ischemic tissue repair.","INSTITUTE":"Renji Hospital, Shanghai Jiao Tong University School of Medicine","LAST_NAME":"Zhan","FIRST_NAME":"Zhenzhen","ADDRESS":"160 Pujian Road, Shanghai, Shanghai, 200127, China","EMAIL":"zhanzz2022@sjtu.edu.cn","PHONE":"86-21-61569249"},

"SUBJECT":{"SUBJECT_TYPE":"Mammal","SUBJECT_SPECIES":"Mus musculus","TAXONOMY_ID":"10090"},
"SUBJECT_SAMPLE_FACTORS":[
{
"Subject ID":"-",
"Sample ID":"G1.1",
"Factors":{"Genotype":"Wild-type"},
"Additional sample data":{"Treatment":"IL-4","RAW_FILE_NAME":"G1.1.mzML"}
},
{
"Subject ID":"-",
"Sample ID":"G1.2",
"Factors":{"Genotype":"Wild-type"},
"Additional sample data":{"Treatment":"IL-4","RAW_FILE_NAME":"G1.2.mzML"}
},
{
"Subject ID":"-",
"Sample ID":"G1.3",
"Factors":{"Genotype":"Wild-type"},
"Additional sample data":{"Treatment":"IL-4","RAW_FILE_NAME":"G1.3.mzML"}
},
{
"Subject ID":"-",
"Sample ID":"G1.4",
"Factors":{"Genotype":"Wild-type"},
"Additional sample data":{"Treatment":"IL-4","RAW_FILE_NAME":"G1.4.mzML"}
},
{
"Subject ID":"-",
"Sample ID":"G1.5",
"Factors":{"Genotype":"Wild-type"},
"Additional sample data":{"Treatment":"IL-4","RAW_FILE_NAME":"G1.5.mzML"}
},
{
"Subject ID":"-",
"Sample ID":"G1.6",
"Factors":{"Genotype":"Wild-type"},
"Additional sample data":{"Treatment":"IL-4","RAW_FILE_NAME":"G1.6.mzML"}
},
{
"Subject ID":"-",
"Sample ID":"G1.7",
"Factors":{"Genotype":"Wild-type"},
"Additional sample data":{"Treatment":"IL-4","RAW_FILE_NAME":"G1.7.mzML"}
},
{
"Subject ID":"-",
"Sample ID":"G1.8",
"Factors":{"Genotype":"Wild-type"},
"Additional sample data":{"Treatment":"IL-4","RAW_FILE_NAME":"G1.8.mzML"}
},
{
"Subject ID":"-",
"Sample ID":"G2.1",
"Factors":{"Genotype":"Npm1-knockout"},
"Additional sample data":{"Treatment":"IL-4","RAW_FILE_NAME":"G2.1.mzML"}
},
{
"Subject ID":"-",
"Sample ID":"G2.2",
"Factors":{"Genotype":"Npm1-knockout"},
"Additional sample data":{"Treatment":"IL-4","RAW_FILE_NAME":"G2.2.mzML"}
},
{
"Subject ID":"-",
"Sample ID":"G2.3",
"Factors":{"Genotype":"Npm1-knockout"},
"Additional sample data":{"Treatment":"IL-4","RAW_FILE_NAME":"G2.3.mzML"}
},
{
"Subject ID":"-",
"Sample ID":"G2.4",
"Factors":{"Genotype":"Npm1-knockout"},
"Additional sample data":{"Treatment":"IL-4","RAW_FILE_NAME":"G2.4.mzML"}
},
{
"Subject ID":"-",
"Sample ID":"G2.5",
"Factors":{"Genotype":"Npm1-knockout"},
"Additional sample data":{"Treatment":"IL-4","RAW_FILE_NAME":"G2.5.mzML"}
},
{
"Subject ID":"-",
"Sample ID":"G2.6",
"Factors":{"Genotype":"Npm1-knockout"},
"Additional sample data":{"Treatment":"IL-4","RAW_FILE_NAME":"G2.6.mzML"}
},
{
"Subject ID":"-",
"Sample ID":"G2.7",
"Factors":{"Genotype":"Npm1-knockout"},
"Additional sample data":{"Treatment":"IL-4","RAW_FILE_NAME":"G2.7.mzML"}
},
{
"Subject ID":"-",
"Sample ID":"G2.8",
"Factors":{"Genotype":"Npm1-knockout"},
"Additional sample data":{"Treatment":"IL-4","RAW_FILE_NAME":"G2.8.mzML"}
}
],
"COLLECTION":{"COLLECTION_SUMMARY":"BMDMs (1×10^7) were induced toward the reparative phenotype using recombinant murine IL-4.BMDMs were counted and washed with PBS.","SAMPLE_TYPE":"Bone marrow"},

"TREATMENT":{"TREATMENT_SUMMARY":"mouse bone marrow cells were flushed out from mouse femurs using RPMI 1640 and cultured in a complete RPMI 1640 medium (added with 10% fetal bovine serum and 1% penicillin/streptomycin) supplemented with 20 ng/ml M-CSF for 7 days to generate mature BMDMs. BMDMs were stimulated with 20 ng/ml IL-4 for 12 h to induce a reparative phenotype."},

"SAMPLEPREP":{"SAMPLEPREP_SUMMARY":"For each sample, 500 μl precooled MeOH/H2O (3/1, v/v) was added in. The samples were then vortexed for 30 sec. After precooling in dry ice, the samples were frozen and thawed three times in liquid nitrogen. They were then vortexed for 30 sec and sonicated for 15 min in an ice-water bath. Next, as a subsequent step, the samples were incubated at -40 °C for 1 h and centrifuged at 12000 rpm for 15 min at 4 °C (RCF = 13800 g, R = 8.6 cm). Afterwards, an aliquot of 400 μl of clear supernatant was collected and dried by spinning. As a reconstitution solution, mix 200 ml of ultrapure water with the residue. The reconstituted samples were vortexed before passing through the filter of the centrifuge tube, after which they were transferred to inserts in injection vials for HPLC-MS/MS analysis."},

"CHROMATOGRAPHY":{"CHROMATOGRAPHY_TYPE":"Ion exchange","INSTRUMENT_NAME":"Thermo Scientific Dionex ICS-6000 HPIC","COLUMN_NAME":"Dionex IonPac AS11-HC (250 × 2mm, 9um, 2000Ã) and AG11-HC (50 × 2 mm, 13um)","SOLVENT_A":"100 mM NaOH/water; water","SOLVENT_B":"water","SOLVENT_C":"2 mM acetic acid in methanol","FLOW_GRADIENT":"0.15 mL/min: 0min:20%/80%, 3.01min:20%/80%,12min:50%/50%, 21min:100%/100%, 26min:100%/100%, 26.1min:20%/80%,0min:80%/20%, 30min:80%/20%","FLOW_RATE":"0.15 mL/min","COLUMN_TEMPERATURE":"30 ℃"},

"ANALYSIS":{"ANALYSIS_TYPE":"MS"},

"MS":{"INSTRUMENT_NAME":"ABI Sciex 6500+","INSTRUMENT_TYPE":"QTRAP","MS_TYPE":"ESI","ION_MODE":"UNSPECIFIED","MS_COMMENTS":"AB SCIEX Analyst Work Station Software (1.6.3 AB SCIEX), MultiQuant 3.0.3.software and Chromeleon7 were employed for MRM data acquisition and processing."},

"MS_METABOLITE_DATA":{
"Units":"pmol",

"Data":[{"Metabolite":"2-Hydroxyglutaric acid","G1.1":"353.56935","G1.2":"286.052825","G1.3":"272.18305","G1.4":"282.9775","G1.5":"239.459175","G1.6":"231.02065","G1.7":"424.797925","G1.8":"340.7256","G2.1":"228.3125088","G2.2":"221.9584252","G2.3":"236.7526931","G2.4":"288.9175097","G2.5":"299.9956142","G2.6":"249.488631","G2.7":"283.1123649","G2.8":"319.2852282"},{"Metabolite":"2-Isopropylmalic acid","G1.1":"0.9983","G1.2":"2.22485","G1.3":"1.34145","G1.4":"2.02645","G1.5":"1.675625","G1.6":"1.95355","G1.7":"2.642275","G1.8":"1.66305","G2.1":"2.508801918","G2.2":"2.447072767","G2.3":"2.43124356","G2.4":"2.641776648","G2.5":"2.583294632","G2.6":"2.535454724","G2.7":"1.931518668","G2.8":"2.819041185"},{"Metabolite":"3-Phosphoglyceric acid","G1.1":"662.716875","G1.2":"602.808875","G1.3":"572.15265","G1.4":"524.734","G1.5":"463.308625","G1.6":"635.65915","G1.7":"676.59855","G1.8":"506.1987","G2.1":"1371.690532","G2.2":"1240.373248","G2.3":"1363.876254","G2.4":"964.2621714","G2.5":"675.422913","G2.6":"714.2932569","G2.7":"982.1174217","G2.8":"891.5440832"},{"Metabolite":"4-Hydroxyphenylpyruvic acid","G1.1":"81.3248","G1.2":"96.284175","G1.3":"93.56405","G1.4":"84.309575","G1.5":"99.989375","G1.6":"94.12045","G1.7":"87.942","G1.8":"91.0897","G2.1":"122.1228408","G2.2":"98.08766312","G2.3":"61.52194483","G2.4":"41.64580102","G2.5":"34.27246617","G2.6":"49.44260163","G2.7":"69.91575844","G2.8":"76.30630261"},{"Metabolite":"6-Phosphogluconic acid","G1.1":"240.5202","G1.2":"118.50755","G1.3":"94.94755","G1.4":"96.25725","G1.5":"208.67475","G1.6":"299.734575","G1.7":"61.190625","G1.8":"29.993825","G2.1":"2089.805422","G2.2":"1126.168022","G2.3":"2045.773817","G2.4":"1550.940041","G2.5":"1189.820744","G2.6":"618.0879713","G2.7":"698.801929","G2.8":"654.9214016"},{"Metabolite":"Adenosine diphosphate","G1.1":"2114.284425","G1.2":"36.3303","G1.3":"22.5604","G1.4":"1305.470875","G1.5":"1001.837425","G1.6":"27.086525","G1.7":"2846.81925","G1.8":"768.754775","G2.1":"246.5635409","G2.2":"150.6115049","G2.3":"158.577258","G2.4":"210.3189543","G2.5":"230.9646058","G2.6":"154.7419443","G2.7":"208.1178201","G2.8":"176.8085079"},{"Metabolite":"Adenosine triphosphate","G1.1":"1151.344325","G1.2":"","G1.3":"","G1.4":"451.242","G1.5":"306.68855","G1.6":"32.6418","G1.7":"955.101","G1.8":"383.8435","G2.1":"29.38434418","G2.2":"16.81402494","G2.3":"18.35588701","G2.4":"29.57093422","G2.5":"52.61077333","G2.6":"11.55427639","G2.7":"19.51661074","G2.8":"13.16886605"},{"Metabolite":"alpha-Ketoglutaric acid","G1.1":"46.966425","G1.2":"42.69085","G1.3":"28.317425","G1.4":"22.268875","G1.5":"30.4687","G1.6":"17.7156","G1.7":"24.656575","G1.8":"54.2128","G2.1":"104.7069477","G2.2":"227.8293493","G2.3":"324.8937877","G2.4":"427.5779611","G2.5":"161.9903358","G2.6":"226.5613289","G2.7":"310.6725448","G2.8":"105.3285927"},{"Metabolite":"alpha-Ketoisovaleric acid","G1.1":"25.363475","G1.2":"22.25485","G1.3":"16.678125","G1.4":"17.108875","G1.5":"18.335575","G1.6":"15.863825","G1.7":"16.758275","G1.8":"18.602575","G2.1":"56.8180971","G2.2":"67.32409939","G2.3":"153.3764063","G2.4":"115.7874865","G2.5":"73.55982223","G2.6":"86.6117799","G2.7":"69.80701848","G2.8":"56.48264555"},{"Metabolite":"cis-Aconitic acid","G1.1":"142.72585","G1.2":"78.073575","G1.3":"59.35565","G1.4":"61.885475","G1.5":"60.217275","G1.6":"73.642875","G1.7":"54.870625","G1.8":"67.2949","G2.1":"44.1868953","G2.2":"47.08093876","G2.3":"42.9280689","G2.4":"48.77017857","G2.5":"63.2198374","G2.6":"41.3403344","G2.7":"47.48063324","G2.8":"62.95108537"},{"Metabolite":"Citric acid","G1.1":"10861.06818","G1.2":"6320.028325","G1.3":"5254.9558","G1.4":"5318.3068","G1.5":"5790.589625","G1.6":"5948.684775","G1.7":"4920.32775","G1.8":"5889.8297","G2.1":"1470.137788","G2.2":"1822.039889","G2.3":"1617.594548","G2.4":"1797.215163","G2.5":"2019.103682","G2.6":"1612.570293","G2.7":"2045.675616","G2.8":"2598.775782"},{"Metabolite":"Cyclic 3'',5''-AMP","G1.1":"2.4274","G1.2":"2.700175","G1.3":"1.7547","G1.4":"2.82715","G1.5":"2.063525","G1.6":"1.476475","G1.7":"3.1914","G1.8":"2.3114","G2.1":"1.127090398","G2.2":"0.559968002","G2.3":"0.588582005","G2.4":"0.872871475","G2.5":"0.918696002","G2.6":"0.773300193","G2.7":"0.775131339","G2.8":"0.825607328"},{"Metabolite":"Cysteic acid","G1.1":"23.6559","G1.2":"34.77775","G1.3":"24.7695","G1.4":"19.988625","G1.5":"38.222225","G1.6":"36.519525","G1.7":"27.833225","G1.8":"22.15095","G2.1":"22.4449095","G2.2":"29.59169437","G2.3":"26.2065915","G2.4":"19.62099371","G2.5":"20.02688361","G2.6":"17.44688431","G2.7":"22.95097957","G2.8":"21.12511369"},{"Metabolite":"Dihydroxyacetone phosphate","G1.1":"108.8036","G1.2":"5.231825","G1.3":"1.517725","G1.4":"8.479625","G1.5":"","G1.6":"","G1.7":"44.45085","G1.8":"52.816875","G2.1":"3.947717022","G2.2":"2.130330769","G2.3":"1.720359284","G2.4":"3.016108964","G2.5":"2.877974999","G2.6":"2.121851729","G2.7":"1.211040243","G2.8":"2.45501641"},{"Metabolite":"Erythrose 4-phosphate","G1.1":"164.9558617","G1.2":"90.29594313","G1.3":"82.66809379","G1.4":"77.01068887","G1.5":"152.3298416","G1.6":"203.9690535","G1.7":"","G1.8":"","G2.1":"2188.542304","G2.2":"1250.506069","G2.3":"2124.983126","G2.4":"1591.68055","G2.5":"1220.954712","G2.6":"736.7872766","G2.7":"803.2816613","G2.8":"775.5216648"},{"Metabolite":"Flavin mononucleotide","G1.1":"204.208275","G1.2":"104.122475","G1.3":"141.716","G1.4":"141.9549","G1.5":"63.9606","G1.6":"118.497375","G1.7":"75.5057","G1.8":"76.8903","G2.1":"11.59709649","G2.2":"20.51669325","G2.3":"27.35110786","G2.4":"26.99196743","G2.5":"11.00362564","G2.6":"28.48225136","G2.7":"24.82489916","G2.8":"23.28291556"},{"Metabolite":"Flavine-adenine dinucleotide","G1.1":"21.019175","G1.2":"75.79235","G1.3":"36.160925","G1.4":"43.942825","G1.5":"68.763325","G1.6":"29.230475","G1.7":"67.936625","G1.8":"61.2197","G2.1":"53.81868162","G2.2":"42.27086514","G2.3":"41.62153622","G2.4":"47.54833205","G2.5":"50.839745","G2.6":"28.55180846","G2.7":"38.99803906","G2.8":"33.06660901"},{"Metabolite":"Fructose 1,6-bisphosphate","G1.1":"611.176075","G1.2":"36.5929","G1.3":"14.30885","G1.4":"49.965075","G1.5":"","G1.6":"12.2116","G1.7":"251.541975","G1.8":"304.56045","G2.1":"94.06668043","G2.2":"87.10310608","G2.3":"83.70723932","G2.4":"96.03520149","G2.5":"94.61920482","G2.6":"83.93948939","G2.7":"67.56516889","G2.8":"100.1152065"},{"Metabolite":"Fructose 6-phosphate","G1.1":"155.41775","G1.2":"141.25605","G1.3":"105.980425","G1.4":"77.46165","G1.5":"120.2498","G1.6":"113.6612","G1.7":"61.217525","G1.8":"48.223225","G2.1":"654.2762055","G2.2":"548.4679478","G2.3":"609.4765139","G2.4":"370.863175","G2.5":"304.065568","G2.6":"571.3848644","G2.7":"408.0286632","G2.8":"674.0641751"},{"Metabolite":"Fumaric acid","G1.1":"960.33405","G1.2":"795.787775","G1.3":"746.7286","G1.4":"768.102225","G1.5":"682.651825","G1.6":"692.1097","G1.7":"633.1991","G1.8":"647.9366","G2.1":"1101.39748","G2.2":"957.8690712","G2.3":"919.5849389","G2.4":"951.0395128","G2.5":"886.1094843","G2.6":"1190.989904","G2.7":"912.0767175","G2.8":"1083.759618"},{"Metabolite":"Glucaric acid","G1.1":"16.189625","G1.2":"19.0524","G1.3":"14.237975","G1.4":"14.0773","G1.5":"15.18215","G1.6":"14.886475","G1.7":"12.81655","G1.8":"12.56145","G2.1":"14.8300905","G2.2":"16.56604766","G2.3":"17.7126193","G2.4":"14.93140445","G2.5":"14.23653405","G2.6":"13.88423373","G2.7":"16.03674149","G2.8":"18.67948891"},{"Metabolite":"Gluconic acid","G1.1":"329.673025","G1.2":"275.73665","G1.3":"276.759625","G1.4":"287.322675","G1.5":"184.49395","G1.6":"186.7567","G1.7":"329.347775","G1.8":"348.3432","G2.1":"163.0151055","G2.2":"190.3151584","G2.3":"175.7770048","G2.4":"204.2375119","G2.5":"210.7861738","G2.6":"145.1066916","G2.7":"173.1577766","G2.8":"175.5066298"},{"Metabolite":"Glucose 6-phosphate","G1.1":"638.693875","G1.2":"283.901","G1.3":"276.5219","G1.4":"255.635025","G1.5":"187.336025","G1.6":"282.099575","G1.7":"217.861225","G1.8":"117.332275","G2.1":"3261.805574","G2.2":"2202.462296","G2.3":"2668.56532","G2.4":"1544.435977","G2.5":"1369.535684","G2.6":"2613.516311","G2.7":"1519.9425","G2.8":"2947.531881"},{"Metabolite":"Glucuronic acid","G1.1":"34.534125","G1.2":"36.766075","G1.3":"30.176075","G1.4":"26.037375","G1.5":"29.171675","G1.6":"31.14115","G1.7":"29.993675","G1.8":"30.099925","G2.1":"14.3474459","G2.2":"19.69507163","G2.3":"18.65107681","G2.4":"18.24964337","G2.5":"22.38355634","G2.6":"18.73940312","G2.7":"20.33668719","G2.8":"24.50621448"},{"Metabolite":"Glyceric acid","G1.1":"67.969925","G1.2":"52.161725","G1.3":"59.43455","G1.4":"49.92865","G1.5":"41.689075","G1.6":"46.06175","G1.7":"49.9907","G1.8":"50.25775","G2.1":"318.3611303","G2.2":"276.2499055","G2.3":"299.8091892","G2.4":"369.4888966","G2.5":"488.0762279","G2.6":"312.6117377","G2.7":"178.3615004","G2.8":"372.39925"},{"Metabolite":"Glycerol 3-phosphate","G1.1":"658.4046","G1.2":"705.461825","G1.3":"699.258325","G1.4":"623.0087","G1.5":"672.65575","G1.6":"749.49825","G1.7":"364.101425","G1.8":"332.71955","G2.1":"756.5621292","G2.2":"600.4178923","G2.3":"549.0117924","G2.4":"450.4658677","G2.5":"432.8545014","G2.6":"346.0969149","G2.7":"565.6869013","G2.8":"395.9758102"},{"Metabolite":"Glycolic acid","G1.1":"59.32735","G1.2":"48.404275","G1.3":"44.45485","G1.4":"33.05985","G1.5":"38.328725","G1.6":"42.4541","G1.7":"49.348775","G1.8":"57.621375","G2.1":"207.0821493","G2.2":"176.7924961","G2.3":"173.3681844","G2.4":"225.9544584","G2.5":"233.6445147","G2.6":"187.0015716","G2.7":"142.6359213","G2.8":"232.1491296"},{"Metabolite":"Glyoxylic acid","G1.1":"49.66495","G1.2":"91.303625","G1.3":"70.2327","G1.4":"62.345575","G1.5":"67.101225","G1.6":"60.5451","G1.7":"61.49945","G1.8":"74.292825","G2.1":"17.04075284","G2.2":"21.79654614","G2.3":"13.21698472","G2.4":"16.74906442","G2.5":"17.87327617","G2.6":"14.94416849","G2.7":"13.03106174","G2.8":"23.65371715"},{"Metabolite":"Guanosine 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"Metabolites":[{"Metabolite":"2-Hydroxyglutaric acid","KEGG ID":"C03196"},{"Metabolite":"2-Isopropylmalic acid","KEGG ID":"C02504"},{"Metabolite":"3-Phosphoglyceric acid","KEGG ID":"C00197"},{"Metabolite":"4-Hydroxyphenylpyruvic acid","KEGG ID":"C01179"},{"Metabolite":"6-Phosphogluconic acid","KEGG ID":"C00345"},{"Metabolite":"Adenosine diphosphate","KEGG ID":"C00008"},{"Metabolite":"Adenosine triphosphate","KEGG ID":"C00002"},{"Metabolite":"alpha-Ketoglutaric acid","KEGG ID":"C00026"},{"Metabolite":"alpha-Ketoisovaleric acid","KEGG ID":"C00141"},{"Metabolite":"cis-Aconitic acid","KEGG ID":"C00417"},{"Metabolite":"Citric acid","KEGG ID":"C00158"},{"Metabolite":"Cyclic 3'',5''-AMP","KEGG ID":"C00575"},{"Metabolite":"Cysteic acid","KEGG ID":"C00506"},{"Metabolite":"Dihydroxyacetone phosphate","KEGG ID":"C00111"},{"Metabolite":"Erythrose 4-phosphate","KEGG ID":"C00279"},{"Metabolite":"Flavin mononucleotide","KEGG ID":"C00061"},{"Metabolite":"Flavine-adenine dinucleotide","KEGG ID":"C00016"},{"Metabolite":"Fructose 1,6-bisphosphate","KEGG ID":"C05378"},{"Metabolite":"Fructose 6-phosphate","KEGG ID":"C00085"},{"Metabolite":"Fumaric acid","KEGG ID":"C00122"},{"Metabolite":"Glucaric acid","KEGG ID":"C00818"},{"Metabolite":"Gluconic acid","KEGG ID":"C00257"},{"Metabolite":"Glucose 6-phosphate","KEGG ID":"C00668"},{"Metabolite":"Glucuronic acid","KEGG ID":"C00191"},{"Metabolite":"Glyceric acid","KEGG ID":"C00258"},{"Metabolite":"Glycerol 3-phosphate","KEGG ID":"C00093"},{"Metabolite":"Glycolic acid","KEGG ID":"C00160"},{"Metabolite":"Glyoxylic acid","KEGG ID":"C00048"},{"Metabolite":"Guanosine diphosphate","KEGG ID":"C00035"},{"Metabolite":"Guanosine triphosphate","KEGG ID":"C00044"},{"Metabolite":"Hippuric acid","KEGG ID":"C01586"},{"Metabolite":"Homogentisic acid","KEGG ID":"C00544"},{"Metabolite":"Indoleacetic acid","KEGG ID":"C00954"},{"Metabolite":"L-Lactic acid","KEGG ID":"C00186"},{"Metabolite":"Maleic acid","KEGG ID":"C01384"},{"Metabolite":"Malic acid","KEGG ID":"C00149"},{"Metabolite":"Mannose 6-phosphate","KEGG ID":"C00275"},{"Metabolite":"Methylmalonic acid","KEGG ID":"C02170"},{"Metabolite":"Mevalonic acid","KEGG ID":"C00418"},{"Metabolite":"Orotic acid","KEGG ID":"C00295"},{"Metabolite":"Phosphoenolpyruvic acid","KEGG ID":"C00074"},{"Metabolite":"Picolinic acid","KEGG ID":"C10164"},{"Metabolite":"Pyruvic acid","KEGG ID":"C00022"},{"Metabolite":"Quinolinic acid","KEGG ID":"C03722"},{"Metabolite":"Ribose 5-phosphate","KEGG ID":"C00117"},{"Metabolite":"Sedoheptulose 7-phosphate","KEGG ID":"C05382"},{"Metabolite":"Succinic acid","KEGG ID":"C00042"},{"Metabolite":"Ureidopropionic acid","KEGG ID":"C02642"}]
}

}