{
"METABOLOMICS WORKBENCH":{"STUDY_ID":"ST003095","ANALYSIS_ID":"AN005066","VERSION":"1","CREATED_ON":"February 17, 2024, 11:17 pm"},

"PROJECT":{"PROJECT_TITLE":"Probiotic intervention of FMT mice","PROJECT_SUMMARY":"L. plantarum intervention of gestational fecal microbiome transplant germ-free mice","INSTITUTE":"Beijing Sanyuan Foods Co. Ltd","LAST_NAME":"Bin","FIRST_NAME":"Liu","ADDRESS":"8th yingchang street, yinghai distric","EMAIL":"liubin@sanyuan.com.cn","PHONE":"01056306594"},

"STUDY":{"STUDY_TITLE":"probiotic on gestational health of FMT GF mice","STUDY_SUMMARY":"probiotic L.plantarum intervention gestational on FMT GF mice","INSTITUTE":"Beijing Sanyuan Foods Co. Ltd","LAST_NAME":"Liu","FIRST_NAME":"Bin","ADDRESS":"8th yingchang street, yinghai distric","EMAIL":"liubin@sanyuan.com.cn","PHONE":"01056306594"},

"SUBJECT":{"SUBJECT_TYPE":"Mammal","SUBJECT_SPECIES":"Mus musculus","TAXONOMY_ID":"10090","GENDER":"Female"},
"SUBJECT_SAMPLE_FACTORS":[
{
"Subject ID":"-",
"Sample ID":"P220801156",
"Factors":{"Sample source":"serum","Factor":"intervention"},
"Additional sample data":{"RAW_FILE_NAME":"Wangyaru-P220801156.mzML"}
},
{
"Subject ID":"-",
"Sample ID":"P220801157",
"Factors":{"Sample source":"serum","Factor":"intervention"},
"Additional sample data":{"RAW_FILE_NAME":"Wangyaru-P220801157.mzML"}
},
{
"Subject ID":"-",
"Sample ID":"P220801158",
"Factors":{"Sample source":"serum","Factor":"intervention"},
"Additional sample data":{"RAW_FILE_NAME":"Wangyaru-P220801158.mzML"}
},
{
"Subject ID":"-",
"Sample ID":"P220801159",
"Factors":{"Sample source":"serum","Factor":"intervention"},
"Additional sample data":{"RAW_FILE_NAME":"Wangyaru-P220801159.mzML"}
},
{
"Subject ID":"-",
"Sample ID":"P220801160",
"Factors":{"Sample source":"serum","Factor":"intervention"},
"Additional sample data":{"RAW_FILE_NAME":"Wangyaru-P220801160.mzML"}
},
{
"Subject ID":"-",
"Sample ID":"P220801161",
"Factors":{"Sample source":"serum","Factor":"intervention"},
"Additional sample data":{"RAW_FILE_NAME":"Wangyaru-P220801161.mzML"}
},
{
"Subject ID":"-",
"Sample ID":"P220801162",
"Factors":{"Sample source":"serum","Factor":"control"},
"Additional sample data":{"RAW_FILE_NAME":"Wangyaru-P220801162.mzML"}
},
{
"Subject ID":"-",
"Sample ID":"P220801163",
"Factors":{"Sample source":"serum","Factor":"control"},
"Additional sample data":{"RAW_FILE_NAME":"Wangyaru-P220801163.mzML"}
},
{
"Subject ID":"-",
"Sample ID":"P220801164",
"Factors":{"Sample source":"serum","Factor":"control"},
"Additional sample data":{"RAW_FILE_NAME":"Wangyaru-P220801164.mzML"}
},
{
"Subject ID":"-",
"Sample ID":"P220801165",
"Factors":{"Sample source":"serum","Factor":"control"},
"Additional sample data":{"RAW_FILE_NAME":"Wangyaru-P220801165.mzML"}
}
],
"COLLECTION":{"COLLECTION_SUMMARY":"Blood was collected and centrifuged at 3,000 rpm for 10 min and stored at -80°C environment","SAMPLE_TYPE":"Blood (serum)","STORAGE_CONDITIONS":"-80℃"},

"TREATMENT":{"TREATMENT_SUMMARY":"mice were randomly assigned into two experimental groups, named control and intervention groups, receiving standard chow feeding, and standard chow + L. plantarum"},

"SAMPLEPREP":{"SAMPLEPREP_SUMMARY":"Samples were vortex for 1 min after adding 0.4 mL methanol; then they were centrifuged for 10 min at 12,000 rpm at 4^oC, and the supernatant was dried and resolved using 0.15 mL 2-chloro-l-phenylalanine (4 ppm) solution, and then filtered by 0.22 μm membrane","PROCESSING_STORAGE_CONDITIONS":"4℃","EXTRACT_STORAGE":"-80℃"},

"CHROMATOGRAPHY":{"CHROMATOGRAPHY_TYPE":"LC","INSTRUMENT_NAME":"Thermo Dionex Ultimate 3000","COLUMN_NAME":"Waters ACQUITY UPLC BEH HILIC (100 x 2.1mm,1.7um)","SOLVENT_A":"formic acid in water","SOLVENT_B":"formic acid in acetonitrile","FLOW_GRADIENT":"0~1 min, 80% B; 1~2 min, 80~70% B; 2~2.5 min, 70% B; 2.5~3 min, 70~50% B; 3~3.5 min, 50% B; 3.5~4 min, 50~80% B; 4~6 min, 80% B","FLOW_RATE":"0.4 mL/min","COLUMN_TEMPERATURE":"40"},

"ANALYSIS":{"ANALYSIS_TYPE":"MS"},

"MS":{"INSTRUMENT_NAME":"Thermo Quantiva QQQ","INSTRUMENT_TYPE":"Triple quadrupole","MS_TYPE":"ESI","ION_MODE":"POSITIVE","MS_COMMENTS":"Multiple reaction monitoring (MRM) was employed. The raw data were firstly converted to mzXML format by MSConvert in the ProteoWizard software package (v3.0.8789) (Adusumilli et al., 2017) and processed using XCMS (Domingo-Almenara et al., 2020) for feature detection, retention time correction and alignment."},

"MS_METABOLITE_DATA":{
"Units":"μmol/L",

"Data":[{"Metabolite":"choline","P220801162":"32.459841","P220801163":"28.537908","P220801164":"32.879674","P220801165":"28.777118","P220801160":"27.971415","P220801159":"31.257804","P220801157":"30.744923","P220801161":"36.689412","P220801156":"30.410424","P220801158":"28.887449"},{"Metabolite":"betaine","P220801162":"12.505261","P220801163":"14.050182","P220801164":"20.875248","P220801165":"14.600753","P220801160":"14.204114","P220801159":"18.598236","P220801157":"14.654962","P220801161":"24.629949","P220801156":"10.71293","P220801158":"8.166123"},{"Metabolite":"TMAO","P220801162":"0.481097","P220801163":"0.473859","P220801164":"0.977366","P220801165":"0.573132","P220801160":"0.39303","P220801159":"0.705887","P220801157":"0.394617","P220801161":"0.843455","P220801156":"0.707087","P220801158":"0.617149"},{"Metabolite":"creatinine","P220801162":"9.540114","P220801163":"8.929402","P220801164":"9.287823","P220801165":"7.752601","P220801160":"10.213967","P220801159":"8.506422","P220801157":"7.354962","P220801161":"18.395334","P220801156":"7.909091","P220801158":"8.218319"},{"Metabolite":"carnitine","P220801162":"12.989527","P220801163":"21.4473","P220801164":"26.015385","P220801165":"14.127252","P220801160":"13.243946","P220801159":"19.635126","P220801157":"18.847274","P220801161":"31.612201","P220801156":"13.765528","P220801158":"8.090083"}],

"Metabolites":[{"Metabolite":"choline","retention index":"0","quantified m/z":"104.2","PubChem ID":"305","KEGG ID":"C00114"},{"Metabolite":"betaine","retention index":"0","quantified m/z":"118.1","PubChem ID":"247","KEGG ID":"C00719"},{"Metabolite":"TMAO","retention index":"0","quantified m/z":"75.9","PubChem ID":"1145","KEGG ID":"C01104"},{"Metabolite":"creatinine","retention index":"0","quantified m/z":"114","PubChem ID":"588","KEGG ID":"C00791"},{"Metabolite":"carnitine","retention index":"0","quantified m/z":"162","PubChem ID":"288","KEGG ID":"C00487"}]
}

}