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MB Sample ID: SA054085
Local Sample ID: | BCP02 |
Subject ID: | SU000956 |
Subject Type: | Human |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Age Or Age Range: | 35 - 65 |
Weight Or Weight Range: | 61 - 75 |
Gender: | Female |
Select appropriate tab below to view additional metadata details:
Subject:
Subject ID: | SU000956 |
Subject Type: | Human |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Age Or Age Range: | 35 - 65 |
Weight Or Weight Range: | 61 - 75 |
Gender: | Female |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
BCP02 | SA054085 | FL010094 | Breast cancer patient | Disease Status |
Collection:
Collection ID: | CO000950 |
Collection Summary: | Venous blood samples were taken in the morning after overnight fasting and were collected using K3EDTA tubes |
Sample Type: | Blood |
Collection Method: | Venous |
Collection Frequency: | one |
Volumeoramount Collected: | 5 mL |
Storage Conditions: | -80C |
Additives: | K3EDTA |
Blood Serum Or Plasma: | Plasma |
Treatment:
Treatment ID: | TR000970 |
Treatment Summary: | None |
Human Fasting: | Overnight |
Sample Preparation:
Sampleprep ID: | SP000963 |
Sampleprep Summary: | 40 µL of plasma was deproteinized with cold acetonitrile (1:3,−20 °C), followed by a two-step derivatization: (i) methoximation with O-Methoxyamine hydrochloride in pyridine (15 mg/mL, room temperature, 16 h) followed by (ii) silylation with BSTFA containing 1% TMCS (70 °C, 1 h). |
Processing Method: | Protein precipitation followed by metabolite extraction |
Processing Storage Conditions: | On ice |
Extraction Method: | Acetonitrile |
Sample Derivatization: | Methoximation and silylation |
Combined analysis:
Analysis ID | AN001503 | AN001504 | AN001505 |
---|---|---|---|
Analysis type | MS | MS | MS |
Chromatography type | GC | Reversed phase | Reversed phase |
Chromatography system | Agilent | Agilent 1200 | Agilent 1200 |
Column | Zebron ZB-5MSi capillary GC | Phenomenex Kinetex C18 (150 x 2.1mm,2.6um) | Phenomenex Kinetex C18 (150 x 2.1mm,2.6um) |
MS Type | EI | ESI | ESI |
MS instrument type | Single quadrupole | QTOF | QTOF |
MS instrument name | Agilent 5973 | Agilent 6530 QTOF | Agilent 6530 QTOF |
Ion Mode | POSITIVE | POSITIVE | NEGATIVE |
Units | peak area | peak area | peak area |
Chromatography:
Chromatography ID: | CH001060 |
Chromatography Summary: | Untargeted GC/MS method |
Instrument Name: | Agilent |
Column Name: | Zebron ZB-5MSi capillary GC |
Column Temperature: | 0-1 min 60ªC 1-33 min to 320ªC |
Flow Rate: | 1.0 mL/min |
Sample Injection: | 2 uL |
Chromatography Type: | GC |
Chromatography ID: | CH001061 |
Chromatography Summary: | Untargeted LC/MS method |
Chromatography Comments: | HPLC system 1200 series coupled to Q-TOF 6520 |
Instrument Name: | Agilent 1200 |
Column Name: | Phenomenex Kinetex C18 (150 x 2.1mm,2.6um) |
Column Pressure: | 300 bar |
Column Temperature: | 40°C |
Flow Gradient: | 25% B increased then to 95% B in 35 min, returned to initial conditions in 1 min and was kept constant for 9 min to ensure re-equilibration of the column |
Flow Rate: | 0.3 mL/min |
Sample Injection: | 10 uL |
Solvent A: | 100% water; 0.1% formic acid |
Solvent B: | 100% acetonitrile; 0.1% formic acid |
Chromatography Type: | Reversed phase |
MS:
MS ID: | MS001386 |
Analysis ID: | AN001503 |
Instrument Name: | Agilent 5973 |
Instrument Type: | Single quadrupole |
MS Type: | EI |
Ion Mode: | POSITIVE |
Helium Flow: | 1.0 mL/min |
Ion Source Temperature: | 150 C |
Ionization: | Electron ionization |
Ionization Energy: | 70 eV |
Source Temperature: | 230 ºC |
Scan Range Moverz: | 50 - 600 |
Scanning: | 1.38 scan/s |
MS ID: | MS001387 |
Analysis ID: | AN001504 |
Instrument Name: | Agilent 6530 QTOF |
Instrument Type: | QTOF |
MS Type: | ESI |
Ion Mode: | POSITIVE |
Capillary Voltage: | 3500 V |
Scan Range Moverz: | 100 / 1200 m/z |
Scanning: | 1.38 scan/s |
MS ID: | MS001388 |
Analysis ID: | AN001505 |
Instrument Name: | Agilent 6530 QTOF |
Instrument Type: | QTOF |
MS Type: | ESI |
Ion Mode: | NEGATIVE |
Capillary Voltage: | 4000 V |
Scan Range Moverz: | 100 / 1200 m/z |
Scanning: | 1.38 scan/s |