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MB Sample ID: SA071550
Local Sample ID: | CARKO_DMSO_48H_103 |
Subject ID: | SU001097 |
Subject Type: | Mammal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Genotype Strain: | Wild Type C57BL/6 and CAR Knockout |
Age Or Age Range: | Approximately 8 weeks old |
Gender: | Male |
Animal Light Cycle: | 12 h |
Animal Feed: | ad libitum |
Animal Water: | ad libitum |
Select appropriate tab below to view additional metadata details:
Subject:
Subject ID: | SU001097 |
Subject Type: | Mammal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Genotype Strain: | Wild Type C57BL/6 and CAR Knockout |
Age Or Age Range: | Approximately 8 weeks old |
Gender: | Male |
Animal Light Cycle: | 12 h |
Animal Feed: | ad libitum |
Animal Water: | ad libitum |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
CARKO_DMSO_48H_103 | SA071550 | FL011216 | CARKO | Genotype |
CARKO_DMSO_48H_103 | SA071550 | FL011216 | 48 | Time Point (h) |
CARKO_DMSO_48H_103 | SA071550 | FL011216 | - | TCPOBOP (mg/kg) |
Collection:
Collection ID: | CO001091 |
Collection Summary: | Liver tissue was snap-frozen in liquid nitrogen and stored at -80C. |
Sample Type: | Liver |
Storage Conditions: | -80℃ |
Treatment:
Treatment ID: | TR001111 |
Treatment Summary: | Each mouse was treated with either a single dose of 2 mg/kg of TCPOBOP or the vehicle control via intraperitoneal injection for either 48 or 72h. |
Sample Preparation:
Sampleprep ID: | SP001104 |
Sampleprep Summary: | Liver tissue was homogenized in a methanol-water mixture, dried and resuspended in phosphate buffer containing TSP-d4. |
Sampleprep Protocol Filename: | GCMS_FattyAcid_protocol.pdf |
Processing Method: | Homogenization |
Combined analysis:
Analysis ID | AN001724 |
---|---|
Analysis type | MS |
Chromatography type | GC |
Chromatography system | Agilent 7890A |
Column | Agilent HP-5MS |
MS Type | EI |
MS instrument type | Single quadrupole |
MS instrument name | Agilent 5975C |
Ion Mode | POSITIVE |
Units | Peak Area |
Chromatography:
Chromatography ID: | CH001218 |
Instrument Name: | Agilent 7890A |
Column Name: | Agilent HP-5MS |
Chromatography Type: | GC |
MS:
MS ID: | MS001594 |
Analysis ID: | AN001724 |
Instrument Name: | Agilent 5975C |
Instrument Type: | Single quadrupole |
MS Type: | EI |
Ion Mode: | POSITIVE |