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MB Sample ID: SA071891
Local Sample ID: | Adipose_54_aged |
Subject ID: | SU001107 |
Subject Type: | Mammal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Genotype Strain: | C57BL/6J mice |
Age Or Age Range: | 8 week (“young”) and 112 week (“old”). |
Weight Or Weight Range: | young: 20.2g (mean), aged: 34.3g (mean) |
Gender: | Male |
Animal Animal Supplier: | CLEA (Tokyo, Japan) |
Animal Light Cycle: | a 12-hour light/12-hour dark schedule |
Animal Feed: | CE-2, CLEA |
Animal Water: | tap water |
Select appropriate tab below to view additional metadata details:
Subject:
Subject ID: | SU001107 |
Subject Type: | Mammal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Genotype Strain: | C57BL/6J mice |
Age Or Age Range: | 8 week (“young”) and 112 week (“old”). |
Weight Or Weight Range: | young: 20.2g (mean), aged: 34.3g (mean) |
Gender: | Male |
Animal Animal Supplier: | CLEA (Tokyo, Japan) |
Animal Light Cycle: | a 12-hour light/12-hour dark schedule |
Animal Feed: | CE-2, CLEA |
Animal Water: | tap water |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
Adipose_54_aged | SA071891 | FL011270 | Aged 112 weeks | Age |
Adipose_54_aged | SA071891 | FL011270 | Epididymal adipose tissue | Tissue |
Collection:
Collection ID: | CO001101 |
Collection Summary: | All mice were killed by decapitation for sample collection. Collected tissues were immediately frozen by liquid nitrogen then stored -80 C until use. (Femoral, Cortex, Liver, Adipose) tissues. |
Sample Type: | Adipose tissue |
Storage Conditions: | -80℃ |
Treatment:
Treatment ID: | TR001121 |
Treatment Summary: | Tissues were homogenised in isopropanol (100 mg/mL) for lipidomics. |
Sample Preparation:
Sampleprep ID: | SP001114 |
Sampleprep Summary: | Tissues were homogenised in isopropanol (100 mg/mL) for lipidomics. |
Combined analysis:
Analysis ID | AN001739 | AN001740 |
---|---|---|
Analysis type | MS | MS |
Chromatography type | Reversed phase | Reversed phase |
Chromatography system | Orbitrap XL | Orbitrap XL |
Column | Waters XBridge C18 (50 x 2.1mm,3um) | Waters XBridge C18 (50 x 2.1mm,3um) |
MS Type | ESI | ESI |
MS instrument type | Single quadrupole | Single quadrupole |
MS instrument name | Thermo LTQ XL | Thermo LTQ XL |
Ion Mode | POSITIVE | NEGATIVE |
Units | peak area | peak area |
Chromatography:
Chromatography ID: | CH001230 |
Chromatography Summary: | The liquid chromatography/mass spectrometry system consisted of an Ultimate3000 RSLC system (Thermo Fisher Scientific inc., Sunnyvale, CA, USA) using a gradient elution consisting of mobile phase A (0.01% acetic acid and 1 mM ammonia in MilliQ water) and mobile phase B (0.001% acetic acid and 0.2 mM ammonia in ethanol/isopropanol [3:1]) |
Instrument Name: | Orbitrap XL |
Column Name: | Waters XBridge C18 (50 x 2.1mm,3um) |
Solvent A: | 100% water; 0.01% acetic acid; 1 mM ammonia |
Solvent B: | 75% ethanol/25% isopropanol; 0.001% acetic acid; 0.2 mM ammonia |
Chromatography Type: | Reversed phase |
MS:
MS ID: | MS001608 |
Analysis ID: | AN001739 |
Instrument Name: | Thermo LTQ XL |
Instrument Type: | Single quadrupole |
MS Type: | ESI |
Ion Mode: | POSITIVE |
MS ID: | MS001609 |
Analysis ID: | AN001740 |
Instrument Name: | Thermo LTQ XL |
Instrument Type: | Single quadrupole |
MS Type: | ESI |
Ion Mode: | NEGATIVE |