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MB Sample ID: SA187092
Local Sample ID: | IJ009_182 |
Subject ID: | SU002082 |
Subject Type: | Human |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Gender: | Not applicable |
Select appropriate tab below to view additional metadata details:
Subject:
Subject ID: | SU002082 |
Subject Type: | Human |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Gender: | Not applicable |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
IJ009_182 | SA187092 | FL023124 | case | treatment |
Collection:
Collection ID: | CO002075 |
Collection Summary: | Plasma was collected and frozen at -80. |
Sample Type: | Blood (serum) |
Treatment:
Treatment ID: | TR002094 |
Treatment Summary: | Blinded study of control versus cases. |
Sample Preparation:
Sampleprep ID: | SP002088 |
Sampleprep Summary: | 1 Thaw plasma samples on ice 2 Vortex plasma sample and pipette 50µl in polypropylene Eppendorf tube/Plate 3 Add Surrogate Standards of the 3 lipid classes (as needed) and other reagents as in the following table: Plasma Sample 10x Oxylipid Surrogate STDs CUDA + PHAU Anti-oxidant ACN/MeOH SUM 4 Vortex for 30sec 5 Centrifuge at 6°C for 5 min at 15,000 g (or 1,800 g for plates) 6 Take the supernatant (~240µl) into new Eppendorf tube/filter plate 7 Spin Filter (0.1 um) or plate filter (0.2um) for 5 min @ 12,000 g (1,800g for plate) 8 place aliquotes of samples into vials, or plate, and cap vials or seal plates 9 Store in -20°C until analysis |
Combined analysis:
Analysis ID | AN003264 |
---|---|
Analysis type | MS |
Chromatography type | HILIC |
Chromatography system | Thermo Vanquish |
Column | Waters Acquity BEH Amide (150 x 2.1mm,1.7um) |
MS Type | ESI |
MS instrument type | Triple quadrupole |
MS instrument name | ABI Sciex 4000 QTrap |
Ion Mode | UNSPECIFIED |
Units | nM |
Chromatography:
Chromatography ID: | CH002408 |
Chromatography Summary: | Targeted oxylipin analysis |
Instrument Name: | Thermo Vanquish |
Column Name: | Waters Acquity BEH Amide (150 x 2.1mm,1.7um) |
Chromatography Type: | HILIC |
MS:
MS ID: | MS003036 |
Analysis ID: | AN003264 |
Instrument Name: | ABI Sciex 4000 QTrap |
Instrument Type: | Triple quadrupole |
MS Type: | ESI |
MS Comments: | analyzed by a Waters UPLC with a 2.1×150 mm, 1.7 µm Acquity BEH column, ESI (+) and (-) switching and detection by multi-reaction monitoring on an Sciex 6500+ QTRAP mass spectrometer. We validated this method for linearity, limit of detection, limit of quantitation, inter- and intra-day reproducibility, and recovery for all targets, yielding better than 15% relative standard deviation and (spiked) recoveries of greater than 80%. |
Ion Mode: | UNSPECIFIED |