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MB Sample ID: SA244965
Local Sample ID: | MNBAL-32B_1 |
Subject ID: | SU002538 |
Subject Type: | Mammal |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Gender: | Male and female |
Select appropriate tab below to view additional metadata details:
Subject:
Subject ID: | SU002538 |
Subject Type: | Mammal |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Gender: | Male and female |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
MNBAL-32B_1 | SA244965 | FL030693 | Human BALF | Subject |
MNBAL-32B_1 | SA244965 | FL030693 | Negative | treatment |
Collection:
Collection ID: | CO002531 |
Collection Summary: | Human lung specimens were collected and stored in -80'C at Mayo Clinic in Scottsdale, Arizona until they were transferred to Arizona State University for VOCs analysis |
Sample Type: | Bronchoalveolar lavage |
Treatment:
Treatment ID: | TR002550 |
Treatment Summary: | 55 bronchoalveolar lavage fluid (BALF), four bronchial wash, and five sputum samples were provided by Tom Gris at Mayo Clinic, Scottsdale, Arizona. Patient samples were categorized by infecting microbial organisms; Coccidioides, fungal, bacterial, viral, multiple, and, non-infectious using hospital codes and doctors notes. A sample was considered Coccidioides positive if it had a positive serology test or was confirmed by culture. Fungal samples were either Coccidioides positive or had a positive fungal culture or fungal smear. Bacterial samples were identified by gram stain, bacterial culture, or PCR. Samples were considered viral positive by PCR. If a sample was considered positive for more than one type of infectious organism (ex, bacterial positive and viral positive) it was labeled multiple. Samples that did not fit into an infectious category, as described, they were labeled non-infectious. |
Sample Preparation:
Sampleprep ID: | SP002544 |
Sampleprep Summary: | The BALF samples were allowed to thaw at 4°C overnight, and then split into technical triplicates of 200 μL that were transferred and sealed into sterilized 2 mL GC headspace vials with Supelco® PTFE/silicone septum magnetic screw caps (Sigma-Aldrich®, St. Louis, MO). All samples were stored in GC headspace vials for up to 14 d at -20°C until analyzed. Samples were randomized for analysis. Volatile metabolites sampling was performed by solid phase microextraction (SPME) using a Gerstel® MPS Robotic Pro MultiPurpose autosampler directed by Maestro® software (Gerstel®, Inc., Linthicum, MD). Volatile metabolite analysis was performed by two-dimensional gas chromatography−time-of-flight mass spectrometry (GC×GC–TOFMS) using a LECO® Pegasus® 4D and Agilent® 7890B GC (LECO® Corp., St. Joseph, MI). An external alkane standards mixture (C8 – C20; Sigma-Aldrich®) was sampled multiple times for calculating retention indices (RI). The injection, chromatographic, and mass spectrometric methods for analyzing the alkane standards were the same as for the samples. |
Extraction Method: | Solid-phase microextraction (SPME) |
Combined analysis:
Analysis ID | AN003997 |
---|---|
Analysis type | MS |
Chromatography type | GC |
Chromatography system | Agilent 7890B |
Column | Column 1: Rxi-624Sil MS, (60m × 0.25mm × 1.4um); Column 2: Stabilwax, (1m × 0.25mm × 0.5um) |
MS Type | EI |
MS instrument type | GC x GC-TOF |
MS instrument name | Leco Pegasus 4D GCxGC TOF |
Ion Mode | POSITIVE |
Units | Peak areas |
Chromatography:
Chromatography ID: | CH002951 |
Methods Filename: | VF_human_GCxGC_methods.docx |
Instrument Name: | Agilent 7890B |
Column Name: | Column 1: Rxi-624Sil MS, (60m × 0.25mm × 1.4um); Column 2: Stabilwax, (1m × 0.25mm × 0.5um) |
Column Temperature: | 230 |
Flow Gradient: | NA |
Flow Rate: | 2 mL∙min-1 (constant) |
Solvent A: | NA |
Solvent B: | NA |
Chromatography Type: | GC |
MS:
MS ID: | MS003745 |
Analysis ID: | AN003997 |
Instrument Name: | Leco Pegasus 4D GCxGC TOF |
Instrument Type: | GC x GC-TOF |
MS Type: | EI |
MS Comments: | See attached protocol |
Ion Mode: | POSITIVE |
Analysis Protocol File: | VF_human_MS_methods.docx |