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MB Sample ID: SA258891
Local Sample ID: | 90267013110 |
Subject ID: | SU002681 |
Subject Type: | Mammal |
Subject Species: | Rattus norvegicus |
Taxonomy ID: | 10116 |
Select appropriate tab below to view additional metadata details:
Subject:
Subject ID: | SU002681 |
Subject Type: | Mammal |
Subject Species: | Rattus norvegicus |
Taxonomy ID: | 10116 |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
90267013110 | SA258891 | FL033380 | Training | Group |
90267013110 | SA258891 | FL033380 | 8 weeks of training or control time | Timepoint |
90267013110 | SA258891 | FL033380 | Female | Sex |
Collection:
Collection ID: | CO002674 |
Collection Summary: | - |
Sample Type: | Plasma |
Treatment:
Treatment ID: | TR002693 |
Treatment Summary: | - |
Sample Preparation:
Sampleprep ID: | SP002687 |
Sampleprep Summary: | Homogenized in PBS, isotope IS added, deproteinized |
Sampleprep Protocol Filename: | pass1b_experimental_design_metabolomics.pdf |
Combined analysis:
Analysis ID | AN004250 |
---|---|
Analysis type | MS |
Chromatography type | Reversed phase |
Chromatography system | Waters Acquity UPLC |
Column | Acquity UPLC BEH C8 (150 x 2.1mm, 1.7um) |
MS Type | ESI |
MS instrument type | Triple quadrupole |
MS instrument name | Thermo TSQ Quantiva |
Ion Mode | POSITIVE |
Units | Micromolar |
Chromatography:
Chromatography ID: | CH003156 |
Chromatography Summary: | Analytes were chromatographically separated with 40 minutes LC gradient |
Methods Filename: | pass1b_amines_methods.pdf |
Instrument Name: | Waters Acquity UPLC |
Column Name: | Acquity UPLC BEH C8 (150 x 2.1mm, 1.7um) |
Column Temperature: | 43C |
Flow Gradient: | t=0 min: 99.9%/0.1% (%A/%B) @ 0.4 ml/min | t=1 min: 99.9%/0.1% (%A/%B) @ 0.4 ml/min | t=2 min: 99.9%/0.1% (%A/%B) @ 0.4 ml/min | t=5.5 min: 98.1%/1.9% (%A/%B) @ 0.4 ml/min | t=6.5 min: 98%/2% (%A/%B) @ 0.4 ml/min | t=10 min: 97.5%/2.4% (%A/%B) @ 0.4 ml/min | t=12 min: 96%/4% (%A/%B) @ 0.4 ml/min | t=20 min: 88%/12% (%A/%B) @ 0.4 ml/min | t=27 min: 86.5%/13.5% (%A/%B) @ 0.4 ml/min | t=31 min: 80%/20% (%A/%B) @ 0.4 ml/min | t=32 min: 2%/98% (%A/%B) @ 0.5 ml/min | t=35 min: 2%/98% (%A/%B) @ 0.5 ml/min | t=35.5 min: 99.9%/0.1% (%A/%B) @ 0.4 ml/min | t=39 min: 99.9%/0.1% (%A/%B) @ 0.4 ml/min |
Flow Rate: | 0.4 ml/min; 0.5 ml/min |
Solvent A: | 1% acetonitrile/99% water; 0.1% formic acid |
Solvent B: | 1% water, 99% Acetonitrile |
Chromatography Type: | Reversed phase |
MS:
MS ID: | MS003997 |
Analysis ID: | AN004250 |
Instrument Name: | Thermo TSQ Quantiva |
Instrument Type: | Triple quadrupole |
MS Type: | ESI |
MS Comments: | Multiple reaction monitoring mode (MRM); peak integration, concentrations calculated against multi-point standard curves; |
Ion Mode: | POSITIVE |