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MB Sample ID: SA265840
Local Sample ID: | SampleBlank03 |
Subject ID: | SU002786 |
Subject Type: | Mammal |
Subject Species: | Rattus norvegicus |
Taxonomy ID: | 10116 |
Select appropriate tab below to view additional metadata details:
Subject:
Subject ID: | SU002786 |
Subject Type: | Mammal |
Subject Species: | Rattus norvegicus |
Taxonomy ID: | 10116 |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
SampleBlank03 | SA265840 | FL034792 | QC-Blank | Group |
SampleBlank03 | SA265840 | FL034792 | 0 hour | Timepoint |
SampleBlank03 | SA265840 | FL034792 | - | Sex |
Collection:
Collection ID: | CO002779 |
Collection Summary: | - |
Sample Type: | Brown adipose |
Treatment:
Treatment ID: | TR002795 |
Treatment Summary: | - |
Sample Preparation:
Sampleprep ID: | SP002792 |
Sampleprep Summary: | IPA extraction with three cycles of freeze/thaw(with sonication) |
Sampleprep Protocol Filename: | pass1b_experimental_design_metabolomics.pdf |
Combined analysis:
Analysis ID | AN004357 |
---|---|
Analysis type | MS |
Chromatography type | Reversed phase |
Chromatography system | Vanquish horizon LC |
Column | Thermo Accucore C30 (100 x 2.1mm,2.6um) |
MS Type | ESI |
MS instrument type | Orbitrap |
MS instrument name | Thermo Q Exactive HF hybrid Orbitrap |
Ion Mode | NEGATIVE |
Units | peak area |
Chromatography:
Chromatography ID: | CH003263 |
Chromatography Summary: | global lipid separation |
Methods Filename: | pass1b_lrpneg_lrppos_methods.pdf |
Instrument Name: | Vanquish horizon LC |
Column Name: | Thermo Accucore C30 (100 x 2.1mm,2.6um) |
Column Temperature: | 50C |
Flow Gradient: | t=0 min: 80%/20% (%A/%B) @ 0.4 ml/min | t=1 min: 40%/60% (%A/%B) @ 0.4 ml/min | t=5 min: 30%/70% (%A/%B) @ 0.4 ml/min | t=8 min: 10%/90% (%A/%B) @ 0.4 ml/min | t=8.2 min: 0%/100% (%A/%B) @ 0.4 ml/min | t=10.5 min: 0%/100% (%A/%B) @ 0.4 ml/min | t=10.7 min: 80%/20% (%A/%B) @ 0.4 ml/min | t=12 min: 80%/20% (%A/%B) @ 0.4 ml/min |
Flow Rate: | 0.4 ml/min |
Solvent A: | 40% water/60% acetonitrile; 10 mM ammonium formate; 0.1% formic acid |
Solvent B: | 10% acetonitrile/90% isopropanol; 10 mM ammonium formate; 0.1% formic acid |
Chromatography Type: | Reversed phase |
MS:
MS ID: | MS004104 |
Analysis ID: | AN004357 |
Instrument Name: | Thermo Q Exactive HF hybrid Orbitrap |
Instrument Type: | Orbitrap |
MS Type: | ESI |
Ion Mode: | NEGATIVE |