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MB Sample ID: SA270391
Local Sample ID: | ABC-18887 |
Subject ID: | SU002805 |
Subject Type: | Human |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Select appropriate tab below to view additional metadata details:
Subject:
Subject ID: | SU002805 |
Subject Type: | Human |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
ABC-18887 | SA270391 | FL035192 | Male | Sex |
ABC-18887 | SA270391 | FL035192 | CASE | Diagnosis |
Collection:
Collection ID: | CO002798 |
Collection Summary: | spun inside centrifuge and processed |
Sample Type: | Blood (plasma) |
Treatment:
Treatment ID: | TR002814 |
Treatment Summary: | NA |
Sample Preparation:
Sampleprep ID: | SP002811 |
Sampleprep Summary: | SOP blood-LCGCextract-02252019 |
Sampleprep Protocol Filename: | SOP_blood-LCGCextract-02252019.docx |
Combined analysis:
Analysis ID | AN004376 | AN004377 |
---|---|---|
Analysis type | MS | MS |
Chromatography type | Reversed phase | Reversed phase |
Chromatography system | Agilent 1290 Infinity | Agilent 1290 Infinity |
Column | Waters ACQUITY UPLC BEH C18 (50 x 2.1mm,1.7um) | Waters ACQUITY UPLC BEH C18 (50 x 2.1mm,1.7um) |
MS Type | ESI | ESI |
MS instrument type | QTOF | QTOF |
MS instrument name | Agilent 6546 QTOF | Agilent 6546 QTOF |
Ion Mode | POSITIVE | NEGATIVE |
Units | normalized peak heights | normalized peak heights |
Chromatography:
Chromatography ID: | CH003282 |
Instrument Name: | Agilent 1290 Infinity |
Column Name: | Waters ACQUITY UPLC BEH C18 (50 x 2.1mm,1.7um) |
Column Temperature: | 65°C |
Flow Gradient: | 0 min 15% (B), 0–0.75 min 30% (B), 0.75-0.98 min 48% (B), 0.98-4.00 min 82% (B), 4.00-4.13 min 99% (B), 4.13-4.50 min 99% (B), 4.50-4.58 min 15% (B), 4.58-5.50 min 15% (B) |
Flow Rate: | 0.8 mL/min |
Solvent A: | 60% acetonitrile/40% water; 10 mM ammonium formate; 0.1% formic acid |
Solvent B: | 90% isopropanol/10% acetonitrile; 10 mM ammonium formate; 0.1% formic acid |
Chromatography Type: | Reversed phase |
MS:
MS ID: | MS004125 |
Analysis ID: | AN004376 |
Instrument Name: | Agilent 6546 QTOF |
Instrument Type: | QTOF |
MS Type: | ESI |
MS Comments: | The quadrupole/time-of-flight (QTOF) mass spectrometers are operated with electrospray ionization (ESI) performing full scan in the mass range m/z 65–1700. Agilent 6546 is equipped with a dual JetStream Source. modes producing both unique and complementary spectra. Instrument parameters are as follows (positive mode) Gas Temp 320°C, Gas Flow 8 l/min, Nebulizer 35 psi, Sheath Gas 350°C, Sheath Gas Flow 11, Capillary Voltage 3500 V, Nozzle Voltage 1000V, Fragmentor 175V, Skimmer 65V. Data (both profile and centroid) are collected at a rate of 5 scans per second. In negative ion mode, Gas Temp 360°C, Gas Flow 9 l/min, Fragmentor 120V, with the other parameters identical to positive ion mode. A reference solution generating ions of 121.050 and 922.007 m/z in positive mode and 119.036 and 966.0007 m/z in negative mode, and these are used for continuous mass correction. |
Ion Mode: | POSITIVE |
MS ID: | MS004126 |
Analysis ID: | AN004377 |
Instrument Name: | Agilent 6546 QTOF |
Instrument Type: | QTOF |
MS Type: | ESI |
MS Comments: | The quadrupole/time-of-flight (QTOF) mass spectrometers are operated with electrospray ionization (ESI) performing full scan in the mass range m/z 65–1700. Agilent 6546 is equipped with a dual JetStream Source. modes producing both unique and complementary spectra. Instrument parameters are as follows (positive mode) Gas Temp 320°C, Gas Flow 8 l/min, Nebulizer 35 psi, Sheath Gas 350°C, Sheath Gas Flow 11, Capillary Voltage 3500 V, Nozzle Voltage 1000V, Fragmentor 175V, Skimmer 65V. Data (both profile and centroid) are collected at a rate of 5 scans per second. In negative ion mode, Gas Temp 360°C, Gas Flow 9 l/min, Fragmentor 120V, with the other parameters identical to positive ion mode. A reference solution generating ions of 121.050 and 922.007 m/z in positive mode and 119.036 and 966.0007 m/z in negative mode, and these are used for continuous mass correction. |
Ion Mode: | NEGATIVE |