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MB Sample ID: SA273709
| Local Sample ID: | Raw_SmpID_102 |
| Subject ID: | SU002822 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Gender: | Male and female |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU002822 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Gender: | Male and female |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| Raw_SmpID_102 | SA273709 | FL035373 | RV | LV/RV |
| Raw_SmpID_102 | SA273709 | FL035373 | Donor | Condition |
| Raw_SmpID_102 | SA273709 | FL035373 | M | Sex |
Collection:
| Collection ID: | CO002815 |
| Collection Summary: | Donated human myocardium. Refer to uploaded acquisition methods. |
| Collection Protocol Filename: | LVvsRV_Tissue_aquisition_Methods.docx |
| Sample Type: | Heart |
| Storage Conditions: | Described in summary |
Treatment:
| Treatment ID: | TR002831 |
| Treatment Summary: | Human myocardial tissue from the left ventricle was compared to the right ventricle within three conditions; non-diseased donors, ischaemic cardiomyopathy, and dilated cardiomyopathy, but not between the different conditions. |
| Treatment Protocol Filename: | LVvsRV_Metabolomics_Methods_2020.docx |
Sample Preparation:
| Sampleprep ID: | SP002828 |
| Sampleprep Summary: | Refer to the uploaded methods file. |
| Sampleprep Protocol Filename: | LVvsRV_Metabolomics_Methods_2020.docx |
Combined analysis:
| Analysis ID | AN004405 | AN004406 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | HILIC | HILIC |
| Chromatography system | Agilent 1260 | Agilent 1260 |
| Column | Waters Atlantis HILIC (150 x 2.1mm,3um) | Waters XBridge Amide (100 x 2.1mm,3.5um) |
| MS Type | ESI | ESI |
| MS instrument type | Triple quadrupole | Triple quadrupole |
| MS instrument name | ABI Sciex 5500 QTrap | ABI Sciex 5500 QTrap |
| Ion Mode | POSITIVE | POSITIVE |
| Units | Relative abundance | Relative abundance |
Chromatography:
| Chromatography ID: | CH003306 |
| Chromatography Summary: | HPLC gradient: The HPLC gradient program begins with an initial condition of 5% solvent A and 95% solvent B, with a flow rate of 0.25 mL/min, which is held for 0.5 minutes to establish system equilibration. The gradient then proceeds as follows: at 6 minutes, the mobile phase composition shifts to 60% solvent A and 40% solvent B for 3minutes; at 10 minutes, it changes back to 5% solvent A and 95% solvent B; at 11 minutes, the flow rate increases to 0.4 mL/min while maintaining a composition of 5% solvent A and 95% solvent B for a duration of 12.5 minutes; and at 24.5 minutes, the flow rate decreases back to 0.25 mL/min. The final condition is maintained for 1 minutes to ensure stability before subsequent analyses. |
| Instrument Name: | Agilent 1260 |
| Column Name: | Waters Atlantis HILIC (150 x 2.1mm,3um) |
| Column Temperature: | 40°C |
| Flow Gradient: | HPLC gradient: The HPLC gradient program begins with an initial condition of 5% solvent A and 95% solvent B, with a flow rate of 0.25 mL/min, which is held for 0.5 minutes to establish system equilibration. The gradient then proceeds as follows: at 6 minutes, the mobile phase composition shifts to 60% solvent A and 40% solvent B for 3minutes; at 10 minutes, it changes back to 5% solvent A and 95% solvent B; at 11 minutes, the flow rate increases to 0.4 mL/min while maintaining a composition of 5% solvent A and 95% solvent B for a duration of 12.5 minutes; and at 24.5 minutes, the flow rate decreases back to 0.25 mL/min. |
| Flow Rate: | 0.250 – 0.400 mL/min |
| Solvent A: | 0.1% Formic acid in 10 mM Ammonium Formate (pH ~2.5) |
| Solvent B: | 0.1% Formic Acid in Acetonitrile |
| Chromatography Type: | HILIC |
| Chromatography ID: | CH003307 |
| Chromatography Summary: | HPLC gradient: The HPLC gradient program is initialized with an initial mobile phase composition of 15% solvent A and 85% solvent B at a flow rate of 0.25 mL/min. Over the course of 8 minutes, the mobile phase composition undergoes a transition to 65% solvent A and 35% solvent B. Subsequently, at 8 minutes, the composition shifts to 98% solvent A and 2% solvent B, maintained for 1 minute. The mobile phase reverts back to the initial composition of 15% solvent A and 85% solvent B at 10 minutes. At 12.5 minutes, the flow rate is increased to 0.5 mL/min, while maintaining a constant mobile phase composition of 15% solvent A and 85% solvent B for a period of 2.5 minutes. Finally, at 15 minutes, the flow rate is reduced back to 0.25 mL/min. To ensure system stability, the final condition is maintained for 1 minute prior to subsequent analyses. |
| Instrument Name: | Agilent 1260 |
| Column Name: | Waters XBridge Amide (100 x 2.1mm,3.5um) |
| Column Temperature: | 40°C |
| Flow Gradient: | HPLC gradient: The HPLC gradient program is initialized with an initial mobile phase composition of 15% solvent A and 85% solvent B at a flow rate of 0.25 mL/min. Over the course of 8 minutes, the mobile phase composition undergoes a transition to 65% solvent A and 35% solvent B. Subsequently, at 8 minutes, the composition shifts to 98% solvent A and 2% solvent B, maintained for 1 minute. The mobile phase reverts back to the initial composition of 15% solvent A and 85% solvent B at 10 minutes. At 12.5 minutes, the flow rate is increased to 0.5 mL/min, while maintaining a constant mobile phase composition of 15% solvent A and 85% solvent B for a period of 2.5 minutes. |
| Flow Rate: | 0.250 – 0.400 mL/min |
| Solvent A: | 95:5 H2O:Acetonitrile (v:v) with 20mM Ammonium Acetate and 20mM Ammonium Hydroxide (pH 9.0) |
| Solvent B: | Acetonitrile |
| Chromatography Type: | HILIC |
MS:
| MS ID: | MS004154 |
| Analysis ID: | AN004405 |
| Instrument Name: | ABI Sciex 5500 QTrap |
| Instrument Type: | Triple quadrupole |
| MS Type: | ESI |
| MS Comments: | The analysis software MultiQuant 3.0 (ABSciex) was used for MRM Q1/Q3 peak integration of the raw data files (Analyst software, v.1.6.2; ABSciex). |
| Ion Mode: | POSITIVE |
| Analysis Protocol File: | LVvsRV_Metabolomics_Methods_2020.docx |
| MS ID: | MS004155 |
| Analysis ID: | AN004406 |
| Instrument Name: | ABI Sciex 5500 QTrap |
| Instrument Type: | Triple quadrupole |
| MS Type: | ESI |
| MS Comments: | The analysis software MultiQuant 3.0 (ABSciex) was used for MRM Q1/Q3 peak integration of the raw data files (Analyst software, v.1.6.2; ABSciex). |
| Ion Mode: | POSITIVE |
| Analysis Protocol File: | LVvsRV_Metabolomics_Methods_2020.docx |
