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MB Sample ID: SA307277
Local Sample ID: | NACH-00276 |
Subject ID: | SU002947 |
Subject Type: | Mammal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Select appropriate tab below to view additional metadata details:
Subject:
Subject ID: | SU002947 |
Subject Type: | Mammal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
NACH-00276 | SA307277 | FL036995 | CON | GROUP_ID |
Collection:
Collection ID: | CO002940 |
Collection Summary: | After euthanasia the serum of the mice were collected and sent to Metabolon Inc for UPLC-MS Analysis. |
Sample Type: | Blood (serum) |
Treatment:
Treatment ID: | TR002956 |
Treatment Summary: | The mice were fed either a control diet or a diet supplemented with 10% galactooligosaccharide for 3 weeks. After the 3 weeks had elapsed the mice were euthanized and the colonic contents were collected. |
Sample Preparation:
Sampleprep ID: | SP002953 |
Sampleprep Summary: | The colonic contents were sent to Metabolon who then performed the sample preparation prior to UPLC-MS analysis. |
Combined analysis:
Analysis ID | AN004637 | AN004638 | AN004639 | AN004640 |
---|---|---|---|---|
Analysis type | MS | MS | MS | MS |
Chromatography type | Reversed phase | Reversed phase | Reversed phase | HILIC |
Chromatography system | Waters Acquity | Waters Acquity | Waters Acquity | Waters Acquity |
Column | Waters Acquity BEH C18 (100 x 2mm, 1.7um) | Waters Acquity BEH C18 (100 x 2mm, 1.7um) | Waters Acquity BEH C18 (100 x 2mm, 1.7um) | Waters Acquity BEH Amide (150 x 2.1mm, 1.7um) |
MS Type | ESI | ESI | ESI | ESI |
MS instrument type | Orbitrap | Orbitrap | Orbitrap | Orbitrap |
MS instrument name | Thermo Q Exactive Orbitrap | Thermo Q Exactive Orbitrap | Thermo Q Exactive Orbitrap | Thermo Q Exactive Orbitrap |
Ion Mode | POSITIVE | POSITIVE | NEGATIVE | NEGATIVE |
Units | Relative Abundance | Relative Abundance | Relative Abundance | Relative Abundance |
Chromatography:
Chromatography ID: | CH003491 |
Chromatography Summary: | Low pH polar (LC/MS Pos early) |
Instrument Name: | Waters Acquity |
Column Name: | Waters Acquity BEH C18 (100 x 2mm, 1.7um) |
Column Temperature: | - |
Flow Gradient: | - |
Flow Rate: | - |
Solvent A: | - |
Solvent B: | - |
Chromatography Type: | Reversed phase |
Chromatography ID: | CH003492 |
Chromatography Summary: | Low pH Lipophilic (LC/MS Pos late) |
Instrument Name: | Waters Acquity |
Column Name: | Waters Acquity BEH C18 (100 x 2mm, 1.7um) |
Column Temperature: | - |
Flow Gradient: | - |
Flow Rate: | - |
Solvent A: | - |
Solvent B: | - |
Chromatography Type: | Reversed phase |
Chromatography ID: | CH003493 |
Chromatography Summary: | High pH (LC/MS Neg) |
Instrument Name: | Waters Acquity |
Column Name: | Waters Acquity BEH C18 (100 x 2mm, 1.7um) |
Column Temperature: | - |
Flow Gradient: | - |
Flow Rate: | - |
Solvent A: | - |
Solvent B: | - |
Chromatography Type: | Reversed phase |
Chromatography ID: | CH003494 |
Chromatography Summary: | HILIC (LC/MS Polar Neg) |
Instrument Name: | Waters Acquity |
Column Name: | Waters Acquity BEH Amide (150 x 2.1mm, 1.7um) |
Column Temperature: | - |
Flow Gradient: | - |
Flow Rate: | - |
Solvent A: | - |
Solvent B: | - |
Chromatography Type: | HILIC |
MS:
MS ID: | MS004384 |
Analysis ID: | AN004637 |
Instrument Name: | Thermo Q Exactive Orbitrap |
Instrument Type: | Orbitrap |
MS Type: | ESI |
MS Comments: | Metabolon (LC/MS Pos early) |
Ion Mode: | POSITIVE |
MS ID: | MS004385 |
Analysis ID: | AN004638 |
Instrument Name: | Thermo Q Exactive Orbitrap |
Instrument Type: | Orbitrap |
MS Type: | ESI |
MS Comments: | Metabolon (LC/MS Pos late) |
Ion Mode: | POSITIVE |
MS ID: | MS004386 |
Analysis ID: | AN004639 |
Instrument Name: | Thermo Q Exactive Orbitrap |
Instrument Type: | Orbitrap |
MS Type: | ESI |
MS Comments: | Metabolon (LC/MS Neg) |
Ion Mode: | NEGATIVE |
MS ID: | MS004387 |
Analysis ID: | AN004640 |
Instrument Name: | Thermo Q Exactive Orbitrap |
Instrument Type: | Orbitrap |
MS Type: | ESI |
MS Comments: | Metabolon (LC/MS Polar) |
Ion Mode: | NEGATIVE |