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MB Sample ID: SA313078
Local Sample ID: | PP_C3 |
Subject ID: | SU002981 |
Subject Type: | Water sample |
Select appropriate tab below to view additional metadata details:
Subject:
Subject ID: | SU002981 |
Subject Type: | Water sample |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
PP_C3 | SA313078 | FL037407 | C | Treatment |
Collection:
Collection ID: | CO002974 |
Collection Summary: | Bacteria was grown in the medium containing CTAB. The water sample was collected with a syringe and filtered for storage. |
Sample Type: | Bacterial cells |
Treatment:
Treatment ID: | TR002990 |
Treatment Summary: | Reactors are set under different conditions: composite membrane (MR) or polypropylene membrane (PP). The biofilms were treated under different conditions: A: with "160mg/L CTAB + 3g/L NH4+, low O2 pressure", B: "160mg/L CTAB + 3g/L NH4+, high O2 pressure", C:" 160mg/L CTAB, high O2 pressure", D:"400mg/L CTAB, high O2 pressure". |
Sample Preparation:
Sampleprep ID: | SP002987 |
Sampleprep Summary: | Liquid samples were filtered and stored in refrigerator before assay. Each sample was injected twice: 10 µL for analysis using negative ionization mode and 4 µL for analysis using positive ionization mode |
Combined analysis:
Analysis ID | AN004702 | AN004703 |
---|---|---|
Analysis type | MS | MS |
Chromatography type | HILIC | HILIC |
Chromatography system | Agilent 1290 | Agilent 1290 |
Column | Waters XBridge BEH Amide (150 x 2.1 mm, 2.5um) | Waters XBridge BEH Amide (150 x 2.1 mm, 2.5um) |
MS Type | ESI | ESI |
MS instrument type | Triple quadrupole | Triple quadrupole |
MS instrument name | Agilent 6490 QQQ | Agilent 6490 QQQ |
Ion Mode | POSITIVE | NEGATIVE |
Units | counts per second | counts per second |
Chromatography:
Chromatography ID: | CH003541 |
Chromatography Summary: | LC-MS/MS platform (Agilent 1290 UPLC-6490 QQQ-MS) |
Instrument Name: | Agilent 1290 |
Column Name: | Waters XBridge BEH Amide (150 x 2.1 mm, 2.5um) |
Column Temperature: | 40 |
Flow Gradient: | After the initial 1-min isocratic elution of 90% B, the percentage of Solvent B was gradually decreased to 40% at t = 11 min. The composition of Solvent B was maintained at 40% for 4 min (t=15 min), and then the percentage of B gradually went back to 90%, to prepare for the next injection. |
Flow Rate: | 0.3 mL/min |
Solvent A: | 10 mM ammonium acetate, 10 mM ammonium hydroxide in 95% H2O/5% acetonitrile |
Solvent B: | 10 mM ammonium acetate, 10 mM ammonium hydroxide in 95% acetonitrile/5% H2O |
Chromatography Type: | HILIC |
MS:
MS ID: | MS004448 |
Analysis ID: | AN004702 |
Instrument Name: | Agilent 6490 QQQ |
Instrument Type: | Triple quadrupole |
MS Type: | ESI |
MS Comments: | LC-MS/MS |
Ion Mode: | POSITIVE |
MS ID: | MS004449 |
Analysis ID: | AN004703 |
Instrument Name: | Agilent 6490 QQQ |
Instrument Type: | Triple quadrupole |
MS Type: | ESI |
MS Comments: | LC-MS/MS |
Ion Mode: | NEGATIVE |