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MB Sample ID: SA021299
Local Sample ID: | 23mar12_23-r002.d |
Subject ID: | SU000442 |
Subject Type: | Animal |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Species Group: | Human |
Select appropriate tab below to view additional metadata details:
Combined analysis:
Analysis ID | AN000663 | AN000664 | AN000665 | AN000666 |
---|---|---|---|---|
Analysis type | MS | MS | MS | MS |
Chromatography type | HILIC | HILIC | Reversed phase | Reversed phase |
Chromatography system | Agilent 6220 | Agilent 6220 | Agilent 6220 | Agilent 6220 |
Column | None | None | None | None |
MS Type | ESI | ESI | ESI | ESI |
MS instrument type | TOF | TOF | TOF | TOF |
MS instrument name | Agilent 6220 TOF | Agilent 6220 TOF | Agilent 6220 TOF | Agilent 6220 TOF |
Ion Mode | POSITIVE | NEGATIVE | POSITIVE | NEGATIVE |
Units | intensity | intensity | intensity | intensity |
Chromatography:
Chromatography ID: | CH000480 |
Chromatography Summary: | The liquid chromatography platform consisted of an Acquity UPLC system (Waters, Milford, MA). Plasma metabolite separation was achieved using both hydrophilic interaction chromatography (ethylene-bridged hybrid 2.1 × 150 mm, 1.7 μm; Waters) and reversed-phase liquid chromatography C18 (high-strength silica 2.1 × 150 mm, 1.8 μm; Waters). For each column, the run time was 20 min at a flow rate of 400 μL/min. Reverse-phase chromatography was performed using 99% solvent A (5 mmol/L NH4 acetate, 0.1% formic acid, and 1% acetonitrile) to 100% solvent B (95% acetonitrile with 0.1% formic acid). The gradient was 0 min, 0% B; 1 min, 0% B; 3 min, 5% B; 13.0 min, 100% B; 16 min, 100% B; 16.5 min, 0% B; and 20 min, 0% B. Other LC parameters were injection volume 5 μL and column temperature 50°C. Each sample was injected in triplicate with blank injections between each sample. Quality controls and standards were run at the beginning and end of the sequence. |
Instrument Name: | Agilent 6220 |
Column Name: | None |
Column Temperature: | 50 |
Flow Gradient: | 0 min, 0% B; 1 min, 0% B; 3 min, 5% B; 13.0 min, 100% B; 16 min, 100% B; 16.5 min, 0% B; and 20 min, 0% B. |
Flow Rate: | 400 µL/min |
Solvent A: | 1% acetonitrile/99% water; 0.1% formic acid; 5 mM ammonium acetate |
Solvent B: | 95% acetonitrile/5% water; 0.1% formic acid |
Chromatography Type: | HILIC |
Chromatography ID: | CH000481 |
Chromatography Summary: | The liquid chromatography platform consisted of an Acquity UPLC system (Waters, Milford, MA). Plasma metabolite separation was achieved using both hydrophilic interaction chromatography (ethylene-bridged hybrid 2.1 × 150 mm, 1.7 μm; Waters) and reversed-phase liquid chromatography C18 (high-strength silica 2.1 × 150 mm, 1.8 μm; Waters). For each column, the run time was 20 min at a flow rate of 400 μL/min. Reverse-phase chromatography was performed using 99% solvent A (5 mmol/L NH4 acetate, 0.1% formic acid, and 1% acetonitrile) to 100% solvent B (95% acetonitrile with 0.1% formic acid). The gradient was 0 min, 0% B; 1 min, 0% B; 3 min, 5% B; 13.0 min, 100% B; 16 min, 100% B; 16.5 min, 0% B; and 20 min, 0% B. Other LC parameters were injection volume 5 μL and column temperature 50°C. Each sample was injected in triplicate with blank injections between each sample. Quality controls and standards were run at the beginning and end of the sequence. |
Instrument Name: | Agilent 6220 |
Column Name: | None |
Column Temperature: | 50 |
Flow Gradient: | 0 min, 0% B; 1 min, 0% B; 3 min, 5% B; 13.0 min, 100% B; 16 min, 100% B; 16.5 min, 0% B; and 20 min, 0% B. |
Flow Rate: | 400 µL/min |
Solvent A: | 99% water/1% acetonitrile; 0.1% formic acid; 5 mM ammonium acetate |
Solvent B: | 95% acetonitrile/5% water; 0.1% formic acid |
Chromatography Type: | Reversed phase |