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MB Sample ID: SA026926
Local Sample ID: | 2033035 |
Subject ID: | SU000537 |
Subject Type: | Animal |
Subject Species: | Macaca mulatta |
Taxonomy ID: | 9544 |
Age Or Age Range: | N/A| N/A| ~3years| ~3years| ~3years| ~3years| ~3years |
Weight Or Weight Range: | N/A| N/A| 6.05 kg| 6.05 kg| 6.05 kg| 6.05 kg| 6.05 kg |
Gender: | Male| Male| Male| Male| Male| Male| Male |
Subject Comments: | Transfusion Donor| Reference Animal| Experimental Subject|Experimental Subject| Experimental Subject| Experimental Subject| Experimental Subject |
Species Group: | Mammal |
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Combined analysis:
Analysis ID | AN000788 | AN000789 |
---|---|---|
Analysis type | MS | MS |
Chromatography type | Reversed phase | Reversed phase |
Chromatography system | Thermo QExactive | Thermo QExactive |
Column | Higgins C18 (100 x 2.1mm,5um) | Higgins C18 (100 x 2.1mm,5um) |
MS Type | ESI | ESI |
MS instrument type | Orbitrap | Orbitrap |
MS instrument name | Thermo Q Exactive Orbitrap | Thermo Q Exactive Orbitrap |
Ion Mode | POSITIVE | NEGATIVE |
Units | peak intensity | peak intensity |
Chromatography:
Chromatography ID: | CH000565 |
Chromatography Summary: | untargeted chromatography (C18 column) |
Methods Filename: | Metab_Orbitrap_Setup_v1_May_17_2013.docx |
Chromatography Comments: | Thermo QExactive coupled to CTC PAL HTC Accela1-TMO autosampler |
Instrument Name: | Thermo QExactive |
Column Name: | Higgins C18 (100 x 2.1mm,5um) |
Column Pressure: | 200 Bar (max) |
Column Temperature: | 30 |
Flow Gradient: | A%: 0-10mins = 5%; B%: 0-2mins = 35%, 2-7mins = gradient to 95%, 7-10mins = 95%; C%: 0-2mins = 60%, 2-7mins = gradient to 0%, 7-10mins = 0% |
Flow Rate: | 0-2mins = 350 μL/min, 2-7mins = gradient to 400 μL/min, 7-10mins = 400 μL/min |
Injection Temperature: | 4C |
Internal Standard: | Caffeine, Diethyl-toluamide |
Internal Standard Mt: | 1.5 min; 6-6.6 min |
Sample Injection: | 10μL |
Sampling Cone: | ESI: HESI probe with S-lens combination |
Solvent A: | 100% water; 0.2% formic acid |
Solvent B: | 100% acetonitrile |
Analytical Time: | 10 minutes |
Capillary Voltage: | 4.6 kV |
Oven Temperature: | 45C |
Running Voltage: | 4.6 kV |
Weak Wash Solvent Name: | H20 with 10% methanol |
Weak Wash Volume: | 20μL |
Chromatography Type: | Reversed phase |