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MB Sample ID: SA072051
Local Sample ID: | Adipose_53_young |
Subject ID: | SU001111 |
Subject Type: | Mammal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Genotype Strain: | C57BL/6J mice |
Age Or Age Range: | 8 week (“young”) and 112 week (“old”). |
Weight Or Weight Range: | young: 20.2g (mean), aged: 34.3g (mean) |
Gender: | Male |
Animal Animal Supplier: | CLEA (Tokyo, Japan) |
Animal Light Cycle: | a 12-hour light/12-hour dark schedule |
Animal Feed: | CE-2, CLEA |
Animal Water: | tap water |
Select appropriate tab below to view additional metadata details:
Combined analysis:
Analysis ID | AN001746 | AN001747 |
---|---|---|
Analysis type | MS | MS |
Chromatography type | Reversed phase | Reversed phase |
Chromatography system | Orbitrap XL | Orbitrap XL |
Column | Waters XBridge C18 (50 x 2.1mm,3um) | Waters XBridge C18 (50 x 2.1mm,3um) |
MS Type | ESI | ESI |
MS instrument type | Single quadrupole | Single quadrupole |
MS instrument name | Thermo LTQ XL | Thermo LTQ XL |
Ion Mode | POSITIVE | NEGATIVE |
Units | peak area | peak area |
Chromatography:
Chromatography ID: | CH001233 |
Chromatography Summary: | The liquid chromatography/mass spectrometry system consisted of an Ultimate3000 RSLC system (Thermo Fisher Scientific inc., Sunnyvale, CA, USA) using a gradient elution consisting of mobile phase A (0.01% acetic acid and 1 mM ammonia in MilliQ water) and mobile phase B (0.001% acetic acid and 0.2 mM ammonia in ethanol/isopropanol [3:1]) |
Instrument Name: | Orbitrap XL |
Column Name: | Waters XBridge C18 (50 x 2.1mm,3um) |
Solvent A: | 100% water; 0.01% acetic acid; 1 mM ammonia |
Solvent B: | 75% ethanol/25% isopropanol; 0.001% acetic acid; 0.2 mM ammonia |
Chromatography Type: | Reversed phase |