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MB Sample ID: SA128006
Local Sample ID: | 9038-2-SE |
Subject ID: | SU001594 |
Subject Type: | Human |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Age Or Age Range: | 20-60 |
Weight Or Weight Range: | BMI range: 20-35 |
Gender: | Male and female |
Human Race: | White; American Indian/Alaskan Native; Black/African American |
Human Ethnicity: | Hispanic or Latino; Non-Hispanic or Latino |
Human Trial Type: | Intervention trial |
Human Exclusion Criteria: | Key exclusion criteria included inflammatory bowel disease, celiac disease, or other chronic intestinal disorders; prior bowel resection surgery other than appendectomy; baseline bowel frequency less than every 2 days or greater than 3 times daily; creatinine concentration greater than the upper limit of normal; diabetes mellitus; currently smoking; body mass index (BMI) <18.5 or >35; and use of antibiotics and probiotics in the prior 6 months |
Select appropriate tab below to view additional metadata details:
Combined analysis:
Analysis ID | AN002529 | AN002530 | AN002531 | AN002532 |
---|---|---|---|---|
Analysis type | MS | MS | MS | MS |
Chromatography type | HILIC | Reversed phase | HILIC | Reversed phase |
Chromatography system | Shimadzu Nexera X2 | Shimadzu Nexera X2 | Shimadzu Nexera X2 | Shimadzu Nexera X2 |
Column | Waters Atlantis HILIC (150 x 2mm,3um) | Waters Acquity BEH C8 (100 x 2.1mm,1.7um) | Phenomenex Luna NH2 (150 x 2.1mm,3um) | Waters Acquity T3 (150 x 2.1 mm,1.7um) |
MS Type | ESI | ESI | ESI | ESI |
MS instrument type | Orbitrap | Orbitrap | Orbitrap | Orbitrap |
MS instrument name | Thermo Exactive Plus Orbitrap | Thermo Exactive Plus Orbitrap | Thermo Q Exactive Plus Orbitrap | Thermo Q Exactive Orbitrap |
Ion Mode | POSITIVE | POSITIVE | NEGATIVE | NEGATIVE |
Units | peak area | peak area | peak area | peak area |
Chromatography:
Chromatography ID: | CH001849 |
Chromatography Summary: | HILIC-pos: high resolution and accurate mass profiling of polar metabolites using HILIC and positive ion mode full scan MS |
Instrument Name: | Shimadzu Nexera X2 |
Column Name: | Waters Atlantis HILIC (150 x 2mm,3um) |
Column Temperature: | 30 ℃ |
Flow Gradient: | linear |
Flow Rate: | 250 uL/min |
Injection Temperature: | 4 |
Solvent A: | 100% water; 0.1% formic acid; 10 mM ammonium formate |
Solvent B: | 100% acetonitrile; 0.1% formic acid |
Chromatography Type: | HILIC |
Chromatography ID: | CH001850 |
Chromatography Summary: | C8-pos: high resolution and accurate mass profiling of polar and nonpolar lipids using reversed phase C8 chromatography and positive ion mode full scan MS |
Instrument Name: | Shimadzu Nexera X2 |
Column Name: | Waters Acquity BEH C8 (100 x 2.1mm,1.7um) |
Column Temperature: | 40 ℃ |
Flow Gradient: | linear |
Flow Rate: | 450 uL/min |
Solvent A: | 95% water/5% methanol; 0.1% formic acid; 10 mM ammonium acetate |
Solvent B: | 100% methanol; 0.1% formic acid |
Chromatography Type: | Reversed phase |
Chromatography ID: | CH001851 |
Chromatography Summary: | HILIC-neg: high resolution and accurate mass profiling of polar metabolites using HILIC and negative ion mode full scan MS |
Instrument Name: | Shimadzu Nexera X2 |
Column Name: | Phenomenex Luna NH2 (150 x 2.1mm,3um) |
Column Temperature: | 30 ℃ |
Flow Gradient: | linear |
Flow Rate: | 400 uL/min |
Injection Temperature: | 4 |
Solvent A: | 100% water; 20 mM ammonium acetate, 20 mM ammonium hydroxide |
Solvent B: | 25% methanol/75% acetonitrile; 10 mM ammonium hydroxide |
Chromatography Type: | HILIC |
Chromatography ID: | CH001852 |
Chromatography Summary: | C18-neg: high resolution and accurate mass profiling of metabolites of intermediate polarity using reversed phase C18 chromatography and negative ion mode full scan MS |
Instrument Name: | Shimadzu Nexera X2 |
Column Name: | Waters Acquity T3 (150 x 2.1 mm,1.7um) |
Column Temperature: | 45 ℃ |
Flow Gradient: | linear |
Flow Rate: | 450 uL/min |
Injection Temperature: | 4 |
Solvent A: | 100% water; 0.01% formic acid |
Solvent B: | 100% acetonitrile; 0.01% acetic acid |
Chromatography Type: | Reversed phase |