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MB Sample ID: SA195158
Local Sample ID: | BileAcid_LI_022421_1968 |
Subject ID: | SU002156 |
Subject Type: | Human |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Gender: | Male and female |
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Combined analysis:
Analysis ID | AN003380 |
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Analysis type | MS |
Chromatography type | Reversed phase |
Chromatography system | Thermo Vanquish |
Column | Waters Acquity BEH C18 (100 x 2mm,1.7um) |
MS Type | ESI |
MS instrument type | Triple quadrupole |
MS instrument name | Thermo TSQ Vantage |
Ion Mode | NEGATIVE |
Units | ng/mL |
Chromatography:
Chromatography ID: | CH002498 |
Chromatography Summary: | Samples were analyzed using a Thermofisher Vanquish UHPLC system coupled to a Thermofisher TSQ Altis triple-quadrupole mass spectrometer. An Aquity BEH C18 column (1.7 µm, 2.1 mm×100 mm) with guard column Acquity BEH C18 (1.7 µm, 2.1 mm×5 mm) was used for chromatographic separation with mobile phases of A: LC-MS-grade water with 0.1% formic acid, and B: LC-MS-grade acetonitrile with 0.1% formic acid with a flow rate of 400 µL/min and column temperature of 50 °C. The gradient began at 20% B for 1 min, then shifted to 45% B between 1 and 11 min, then to 95% B between 11 and 14 min, then to 99% B between 14 and 14.5 min, 99% B was maintained until 15.5 min, then transitioned from 99% B to 20% B between 15.5 and 16.5 min, and maintained at 20% B until 18 min. Injection volume was 5 µL and MRM scans were collected for all bile acids and internal standards. |
Instrument Name: | Thermo Vanquish |
Column Name: | Waters Acquity BEH C18 (100 x 2mm,1.7um) |
Column Temperature: | 50 |
Flow Gradient: | The gradient began at 20% B for 1 min, then shifted to 45% B between 1 and 11 min, then to 95% B between 11 and 14 min, then to 99% B between 14 and 14.5 min, 99% B was maintained until 15.5 min, then transitioned from 99% B to 20% B between 15.5 and 16.5 min, and maintained at 20% B until 18 min. |
Flow Rate: | 400 uL/min |
Sample Injection: | 5 ul |
Solvent A: | 100% water; 0.1% formic acid |
Solvent B: | 100% acetonitrile; 0.1% formic acid |
Chromatography Type: | Reversed phase |