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MB Sample ID: SA201545
Local Sample ID: | RPMI_IST_1 |
Subject ID: | SU002189 |
Subject Type: | Cultured cells |
Subject Species: | Homo sapiens |
Genotype Strain: | OVCAR-5 and CaOV3 cell lines |
Gender: | Not applicable |
Cell Biosource Or Supplier: | CaOV3 was purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA). OVCAR-5 cell line obtained from Dr. Thomas Hamilton (Fox Chase Cancer Centre, Philadelphia, PA). |
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Combined analysis:
Analysis ID | AN003439 | AN003440 |
---|---|---|
Analysis type | MS | MS |
Chromatography type | HILIC | HILIC |
Chromatography system | Thermo Dionex Ultimate 3000 RS | Thermo Dionex Ultimate 3000 RS |
Column | SeQuant ZIC-pHILIC (150 x 4.2mm,5um) | SeQuant ZIC-pHILIC (150 x 4.2mm,5um) |
MS Type | ESI | ESI |
MS instrument type | Orbitrap | Orbitrap |
MS instrument name | Thermo Q Exactive Orbitrap | Thermo Q Exactive Orbitrap |
Ion Mode | POSITIVE | NEGATIVE |
Units | peak height | peak height |
Chromatography:
Chromatography ID: | CH002541 |
Chromatography Summary: | LCMS data was acquired on Q-Exactive Orbitrap mass spectrometer (Thermo Fisher) coupled with high-performance liquid chromatography (HPLC) system Dionex Ultimate® 3000 RS (Thermo Fisher). Chromatographic separation was performed on a ZIC-pHILIC column (5 µm, polymeric, 150 × 4.6 mm, SeQuant®, Merck). The mobile phase (A) was 20 mM ammonium carbonate and (B) acetonitrile. The gradient program started at 80% B and was reduced to 50% B over 15 min, then reduced from 50% B to 5% B over 3 min, followed by wash with 5% B for another 3 min, and finally 8 min re-equilibration with 80% B. The flow rate was 0.3 mL/min and column compartment temperature was 40ºC. The total run time was 32 min with an injection sample volume of 10 µL. |
Methods Filename: | Metabolomics_pHILIC_Parkville_v1.meth |
Instrument Name: | Thermo Dionex Ultimate 3000 RS |
Column Name: | SeQuant ZIC-pHILIC (150 x 4.2mm,5um) |
Column Pressure: | 60 bar at starting conditions |
Column Temperature: | 25 C |
Flow Gradient: | 0 min - 80%B, 15 min - 50%B, 18 min - 5%B, 21 min - 5%B, 24 min - 80%B, 32 min - 80%B |
Flow Rate: | 0.3 ml/min |
Injection Temperature: | 4 C |
Internal Standard: | CAPS, CHAPS, PIPES |
Sample Injection: | 10 uL |
Solvent A: | 100% water; 20 mM ammonium formate |
Solvent B: | 100% acetonitrile |
Analytical Time: | 32 min |
Capillary Voltage: | 3.5 kV |
Oven Temperature: | 25 C |
Washing Buffer: | syringe wash 50% IPA |
Weak Wash Solvent Name: | 10% MeOH |
Strong Wash Solvent Name: | 10% MeOH |
Sample Loop Size: | 25 uL |
Sample Syringe Size: | 25 uL |
Chromatography Type: | HILIC |