Return to study ST002239 main page
MB Sample ID: SA214023
Local Sample ID: | 16_EA_1-neg |
Subject ID: | SU002325 |
Subject Type: | Plant |
Subject Species: | Camelina Sativa |
Age Or Age Range: | 10 days after fertilization |
Species Group: | Developing seeds |
Select appropriate tab below to view additional metadata details:
Combined analysis:
Analysis ID | AN003655 | AN003656 |
---|---|---|
Analysis type | MS | MS |
Chromatography type | Reversed phase | Reversed phase |
Chromatography system | Dionex UltiMate 3000 RSLCnano | Dionex UltiMate 3000 RSLCnano |
Column | Custom C8 - Higgins Analytical | Custom C8 - Higgins Analytical |
MS Type | ESI | ESI |
MS instrument type | Orbitrap | Orbitrap |
MS instrument name | Thermo Fusion Tribrid Orbitrap | Thermo Fusion Tribrid Orbitrap |
Ion Mode | POSITIVE | NEGATIVE |
Units | Intensity | Intensity |
Chromatography:
Chromatography ID: | CH002709 |
Chromatography Summary: | Separations for lipidomics were achieved using the loading pump of a Dionex UltiMate 3000 RSLCnano system (Thermo Fisher Scientific) operating at a flow rate of 40 µL min-1 equipped with a custom-made C8 column (100 x 0.5 x 5 µm) from Higgins Analytical Inc. (Mountain view, CA) re-packed from a nucleodur C8 Gravity column (Macherey-Nagel, Allentown, PA). Mobile phases comprised of 1% 1 M ammonium acetate, 0.1 % acetic acid in water (A) and 1% 1 M ammonium acetate, 0.1% acetic acid in 7:3 (v/v) acetonitrile: isopropanol (B). The following gradient modified from a previously described method27 to adapt to micro flow was used; 0-1 min at 55% B, 4 min at 75% B, 12 min at 89% B, 15 min at 99% B, 18 min at 99% B and 20 min at 55% B followed by equilibration up to 30 min. |
Instrument Name: | Dionex UltiMate 3000 RSLCnano |
Column Name: | Custom C8 - Higgins Analytical |
Column Temperature: | 40 |
Flow Rate: | 0.04 mL min-1 |
Internal Standard: | Equisplash |
Solvent A: | 100% water; 0.1% acetic acid; 10 mM ammonium acetate |
Solvent B: | 70% acetonitrile/30% isopropanol; 0.1% acetic acid; 10 mM ammonium acetate |
Chromatography Type: | Reversed phase |