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MB Sample ID: SA236670
Local Sample ID: | TFA-P22 |
Subject ID: | SU002443 |
Subject Type: | Mammal |
Subject Species: | Rattus norvegicus |
Taxonomy ID: | 10116 |
Age Or Age Range: | 26 weeks of age |
Weight Or Weight Range: | 320-460 grams |
Gender: | Male |
Animal Animal Supplier: | RRRC (University of Missouri) |
Animal Housing: | Center for Comparative Medicine UConn |
Animal Feed: | Modified AIN-93G diet from Research Diets |
Animal Water: | ab libitum |
Select appropriate tab below to view additional metadata details:
Combined analysis:
Analysis ID | AN003842 | AN003843 | AN003844 | AN003845 |
---|---|---|---|---|
Analysis type | MS | MS | MS | MS |
Chromatography type | Reversed phase | Reversed phase | Reversed phase | Reversed phase |
Chromatography system | Prominence XR | Prominence XR | Prominence XR | Prominence XR |
Column | Targa C8, 2x10 mm, 5μ | Luna C18 (150 x 2.1mm,3um) | Targa C8, 2x10 mm, 5μ | Luna C18 (150 x 2.1mm,3um) |
MS Type | ESI | ESI | ESI | ESI |
MS instrument type | Triple quadrupole | Triple quadrupole | Triple quadrupole | Triple quadrupole |
MS instrument name | ABI Sciex 5500 QTrap | ABI Sciex 5500 QTrap | ABI Sciex 5500 QTrap | ABI Sciex 5500 QTrap |
Ion Mode | NEGATIVE | NEGATIVE | NEGATIVE | NEGATIVE |
Units | ng/g tissue | mg/mL | ng/mL | mg/g tissue |
Chromatography:
Chromatography ID: | CH002844 |
Chromatography Summary: | HPLC was performed on a Prominence XR system (Shimadzu) using Luna C18 (3μ, 2.1x150 mm) column. The mobile phase consisted of a gradient between A: methanol-water-acetonitrile (10:85:5 v/v) and B: methanol-water-acetonitrile (90:5:5 v/v), both containing 0.1% ammonium acetate. The gradient program with respect to the composition of B was as follows: 0-1 min, 50%; 1-8 min, 50-80%; 8-15 min, 80-95%; and 15-17 min, 95%. The flow rate was 0.2 ml/min. The HPLC eluate was directly introduced to ESI source of QTRAP5500 mass analyzer (ABSCIEX) in the negative ion mode with following conditions: Curtain gas: 35 psi, GS1: 35 psi, GS2: 65 psi, Temperature: 600 ˚C, Ion Spray Voltage: -1500 V, Collision gas: low, Declustering Potential: -60 V, and Entrance Potential: -7 V. The eluate was monitored by Multiple Reaction Monitoring method to detect unique molecular ion – daughter ion combinations for each of the lipid mediators using a scheduled MRM around the expected retention time for each compound. Optimized Collisional Energies (18 – 35 eV) and Collision Cell Exit Potentials (7 – 10 V) were used for each MRM transition. Spectra of each peak detected in the scheduled MRM were recorded using Enhanced Product Ion scan to confirm the structural identity. The data was collected using Analyst 1.6.2 software and the MRM transition chromatograms were quantitated by MultiQuant software (both from ABSCIEX). The internal standard signals in each chromatogram were used for normalization, recovery, as well as relative quantitation of each analyte. |
Instrument Name: | Prominence XR |
Column Name: | Targa C8, 2x10 mm, 5μ |
Flow Rate: | 0.25ml/min |
Chromatography Type: | Reversed phase |
Chromatography ID: | CH002845 |
Chromatography Summary: | HPLC was performed on a Prominence XR system (Shimadzu) using Luna C18 (3μ, 2.1x150 mm) column. The mobile phase consisted of a gradient between A: methanol-water-acetonitrile (10:85:5 v/v) and B: methanol-water-acetonitrile (90:5:5 v/v), both containing 0.1% ammonium acetate. The gradient program with respect to the composition of B was as follows: 0-1 min, 50%; 1-8 min, 50-80%; 8-15 min, 80-95%; and 15-17 min, 95%. The flow rate was 0.2 ml/min. The HPLC eluate was directly introduced to ESI source of QTRAP5500 mass analyzer (ABSCIEX) in the negative ion mode with following conditions: Curtain gas: 35 psi, GS1: 35 psi, GS2: 65 psi, Temperature: 600 ˚C, Ion Spray Voltage: -1500 V, Collision gas: low, Declustering Potential: -60 V, and Entrance Potential: -7 V. The eluate was monitored by Multiple Reaction Monitoring method to detect unique molecular ion – daughter ion combinations for each of the lipid mediators using a scheduled MRM around the expected retention time for each compound. Optimized Collisional Energies (18 – 35 eV) and Collision Cell Exit Potentials (7 – 10 V) were used for each MRM transition. Spectra of each peak detected in the scheduled MRM were recorded using Enhanced Product Ion scan to confirm the structural identity. The data was collected using Analyst 1.6.2 software and the MRM transition chromatograms were quantitated by MultiQuant software (both from ABSCIEX). The internal standard signals in each chromatogram were used for normalization, recovery, as well as relative quantitation of each analyte. |
Instrument Name: | Prominence XR |
Column Name: | Luna C18 (150 x 2.1mm,3um) |
Flow Rate: | 0.2ml/min |
Chromatography Type: | Reversed phase |
Chromatography ID: | CH002846 |
Chromatography Summary: | HPLC was performed on a Prominence XR system (Shimadzu) using Luna C18 (3μ, 2.1x150 mm) column. The mobile phase consisted of a gradient between A: methanol-water-acetonitrile (10:85:5 v/v) and B: methanol-water-acetonitrile (90:5:5 v/v), both containing 0.1% ammonium acetate. The gradient program with respect to the composition of B was as follows: 0-1 min, 50%; 1-8 min, 50-80%; 8-15 min, 80-95%; and 15-17 min, 95%. The flow rate was 0.2 ml/min. The HPLC eluate was directly introduced to ESI source of QTRAP5500 mass analyzer (ABSCIEX) in the negative ion mode with following conditions: Curtain gas: 35 psi, GS1: 35 psi, GS2: 65 psi, Temperature: 600 ˚C, Ion Spray Voltage: -1500 V, Collision gas: low, Declustering Potential: -60 V, and Entrance Potential: -7 V. The eluate was monitored by Multiple Reaction Monitoring method to detect unique molecular ion – daughter ion combinations for each of the lipid mediators using a scheduled MRM around the expected retention time for each compound. Optimized Collisional Energies (18 – 35 eV) and Collision Cell Exit Potentials (7 – 10 V) were used for each MRM transition. Spectra of each peak detected in the scheduled MRM were recorded using Enhanced Product Ion scan to confirm the structural identity. The data was collected using Analyst 1.6.2 software and the MRM transition chromatograms were quantitated by MultiQuant software (both from ABSCIEX). The internal standard signals in each chromatogram were used for normalization, recovery, as well as relative quantitation of each analyte. |
Instrument Name: | Prominence XR |
Column Name: | Targa C8, 2x10 mm, 5μ |
Flow Rate: | 0.25ml/min |
Chromatography Type: | Reversed phase |
Chromatography ID: | CH002847 |
Chromatography Summary: | HPLC was performed on a Prominence XR system (Shimadzu) using Luna C18 (3μ, 2.1x150 mm) column. The mobile phase consisted of a gradient between A: methanol-water-acetonitrile (10:85:5 v/v) and B: methanol-water-acetonitrile (90:5:5 v/v), both containing 0.1% ammonium acetate. The gradient program with respect to the composition of B was as follows: 0-1 min, 50%; 1-8 min, 50-80%; 8-15 min, 80-95%; and 15-17 min, 95%. The flow rate was 0.2 ml/min. The HPLC eluate was directly introduced to ESI source of QTRAP5500 mass analyzer (ABSCIEX) in the negative ion mode with following conditions: Curtain gas: 35 psi, GS1: 35 psi, GS2: 65 psi, Temperature: 600 ˚C, Ion Spray Voltage: -1500 V, Collision gas: low, Declustering Potential: -60 V, and Entrance Potential: -7 V. The eluate was monitored by Multiple Reaction Monitoring method to detect unique molecular ion – daughter ion combinations for each of the lipid mediators using a scheduled MRM around the expected retention time for each compound. Optimized Collisional Energies (18 – 35 eV) and Collision Cell Exit Potentials (7 – 10 V) were used for each MRM transition. Spectra of each peak detected in the scheduled MRM were recorded using Enhanced Product Ion scan to confirm the structural identity. The data was collected using Analyst 1.6.2 software and the MRM transition chromatograms were quantitated by MultiQuant software (both from ABSCIEX). The internal standard signals in each chromatogram were used for normalization, recovery, as well as relative quantitation of each analyte. |
Instrument Name: | Prominence XR |
Column Name: | Luna C18 (150 x 2.1mm,3um) |
Flow Rate: | 0.2ml/min |
Chromatography Type: | Reversed phase |