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MB Sample ID: SA247794
Local Sample ID: | 36-week db/db+atRA-1 |
Subject ID: | SU002564 |
Subject Type: | Mammal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Genotype Strain: | db/m; db/db; db/db+retinol; db/db+retinoic acid; db/db+AAV9-RDH10; RDH10-cKO; RDH10fl/fl |
Age Or Age Range: | 32-week; 36-week; 21-week |
Gender: | Male |
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Combined analysis:
Analysis ID | AN004041 |
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Analysis type | MS |
Chromatography type | Reversed phase |
Chromatography system | Sciex Triple Quad TM 4500MD MS |
Column | Phenomenex Kinetex C18 (50 x 2.1mm,1.7um) |
MS Type | ESI |
MS instrument type | Triple quadrupole |
MS instrument name | ABI Sciex 5500 QTrap |
Ion Mode | POSITIVE |
Units | ng/ml |
Chromatography:
Chromatography ID: | CH002989 |
Instrument Name: | Sciex Triple Quad TM 4500MD MS |
Column Name: | Phenomenex Kinetex C18 (50 x 2.1mm,1.7um) |
Column Temperature: | 25 |
Flow Gradient: | initial conditions 75% B; from 0 to 3.5min linear increase to 95% B; between 3.5 and 4.5min 95% B was retained; at 4.6 min back to initial conditions with 75% B; finally, 75% B was held from 4.6 to 5.9min |
Flow Rate: | 0.2ml/min |
Solvent A: | 40% acetonitrile/30% methanol/30% water; 0.1 % formic acid |
Solvent B: | 55% acetonitrile/30% methanol/15% water; 0.1 % formic acid |
Chromatography Type: | Reversed phase |