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MB Sample ID: SA257735
Local Sample ID: | MCF001027_wt1 |
Subject ID: | SU002662 |
Subject Type: | Fish |
Subject Species: | Danio rerio |
Taxonomy ID: | 7955 |
Genotype Strain: | WT/PMM2 mutant sa10150 |
Age Or Age Range: | 6 dpf |
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Combined analysis:
Analysis ID | AN004221 | AN004222 |
---|---|---|
Analysis type | MS | MS |
Chromatography type | Reversed phase | HILIC |
Chromatography system | Waters Acquity | Agilent Infinity |
Column | C-18: Acquity UPLC -HSS T3 1. 8 µm; 2.1 x 150 mm, Waters | Poroshell 120 HILIC-Z PEEK Column 2.1x 150mm, 5 um |
MS Type | ESI | ESI |
MS instrument type | Orbitrap | Orbitrap |
MS instrument name | Thermo Q Exactive Orbitrap | Thermo Q Exactive Focus |
Ion Mode | NEGATIVE | NEGATIVE |
Units | AUC | AUC |
Chromatography:
Chromatography ID: | CH003130 |
Chromatography Summary: | Reverse phase, IP, C18 column |
Instrument Name: | Waters Acquity |
Column Name: | C-18: Acquity UPLC -HSS T3 1. 8 µm; 2.1 x 150 mm, Waters |
Column Temperature: | 40 |
Flow Gradient: | The gradient started with 5% of solvent B and 95% solvent A and remained at 5% B until 2 min post injection. A linear gradient to 37% B was carried out until 7 min and increased to 41% until 14 min. Between 14 and 26 minutes the gradient increased to 95% of B and remained at 95% B for 4 minutes. At 30 min the gradient returned to 5% B. The chromatography was stopped at 40 min. |
Flow Rate: | 0.25 mL/min |
Solvent A: | 100% water; 10mM tributylamine; 15mM acetic acid |
Solvent B: | 100% methanol |
Chromatography Type: | Reversed phase |
Chromatography ID: | CH003131 |
Chromatography Summary: | HILIC |
Instrument Name: | Agilent Infinity |
Column Name: | Poroshell 120 HILIC-Z PEEK Column 2.1x 150mm, 5 um |
Column Temperature: | 25 |
Flow Gradient: | A linear gradient was carried out starting with 90% solvent A and 10% solvent B. From 2 to 12 min the gradient changed to 60% B. The gradient was kept on 60% B for 3 minutes and followed by a decrease to 10% B. The chromatography was stopped at 25 min |
Flow Rate: | 0.25 mL/min |
Solvent A: | 100% acetonitrile |
Solvent B: | 100% water; 10 mM sodium acetate, pH 9.3 |
Chromatography Type: | HILIC |