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MB Sample ID: SA259017
Local Sample ID: | 90420013108 |
Subject ID: | SU002683 |
Subject Type: | Mammal |
Subject Species: | Rattus norvegicus |
Taxonomy ID: | 10116 |
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Combined analysis:
Analysis ID | AN004252 |
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Analysis type | MS |
Chromatography type | Reversed phase |
Chromatography system | Waters Acquity UPLC |
Column | Waters Acquity UPLC BEH C18 Column, 1.7 µm, 2.1 × 50 mm |
MS Type | ESI |
MS instrument type | Triple quadrupole |
MS instrument name | Waters Xevo TQ-S |
Ion Mode | POSITIVE |
Units | micromole/L |
Chromatography:
Chromatography ID: | CH003158 |
Chromatography Summary: | The analytical column is used at 30°C. 10 µl of the sample are injected onto the column and eluted at a flow rate of 0.4 ml/min. The gradient begins with 45% eluent A (5 mM ammonium acetate in water) and is then programmed as follows: 0 to 2 min – gradient to 55% eluent B (methanol); 2 to 2.5 min - gradient to 95% eluent B; 2.5 to 3.2 min - hold at 95% eluent B, return to 45% A, and re-equilibrate the column at initial conditions for 1 minute. |
Methods Filename: | pass1b_ka_methods.pdf |
Instrument Name: | Waters Acquity UPLC |
Column Name: | Waters Acquity UPLC BEH C18 Column, 1.7 µm, 2.1 × 50 mm |
Column Temperature: | 30C |
Flow Gradient: | 45% A and 55% B for 2 minutes, followed by a linear gradient to 95% B from 2 to 2.5 minutes, held at 95% B for 0.7 minutes, returned to 45% A, and finally the column was re-equilibrated at initial conditions for 1 minute. The total run time was 4.7 minutes. |
Flow Rate: | 0.4 ml/min |
Solvent A: | 100% water; 5 mM ammonium acetate |
Solvent B: | 100% methanol |
Chromatography Type: | Reversed phase |