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MB Sample ID: SA272265
Local Sample ID: | RF213A |
Subject ID: | SU002812 |
Subject Type: | Mammal |
Subject Species: | Mus musculus |
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Combined analysis:
Analysis ID | AN004389 |
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Analysis type | MS |
Chromatography type | Reversed phase |
Chromatography system | Thermo Vanquish |
Column | Ascentis Express C18 (150 x 2.1mm,2.7um) |
MS Type | ESI |
MS instrument type | Orbitrap |
MS instrument name | Thermo Q Exactive Plus Orbitrap |
Ion Mode | POSITIVE |
Units | nM |
Chromatography:
Chromatography ID: | CH003292 |
Chromatography Summary: | Ascentis Express C18 HPLC column (2.7 μm × 15 cm × 2.1 mm; Sigma Aldrich). |
Instrument Name: | Thermo Vanquish |
Column Name: | Ascentis Express C18 (150 x 2.1mm,2.7um) |
Column Temperature: | 30 |
Flow Gradient: | 0–5 min: gradient was held at 5% B; 2–12.1 min: linear gradient of 5% to 95% B; 12.1–17.0 min: 95% B; 17.1–21.0 min: gradient was returned to 5% B |
Flow Rate: | 0.250 ml/min |
Solvent A: | water, 0.1% formic acid |
Solvent B: | acetonitrile, 0.1% formic acid |
Chromatography Type: | Reversed phase |