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MB Sample ID: SA013413
Local Sample ID: | B3P403 |
Subject ID: | SU000314 |
Subject Type: | Insect |
Subject Species: | Sarcophaga crassipalpis |
Taxonomy ID: | 59312 |
Genotype Strain: | Lab colony |
Age Or Age Range: | 15-25 day pupae |
Weight Or Weight Range: | 110-130 mg |
Animal Animal Supplier: | lab colony |
Animal Housing: | sterilite containers |
Animal Light Cycle: | 10:14 L:D |
Animal Feed: | beef liver |
Animal Inclusion Criteria: | must be in diapause |
Species Group: | Insect |
Select appropriate tab below to view additional metadata details:
Collection:
Collection ID: | CO000308 |
Collection Summary: | Pupae were sampled at the appropriate time point and flash frozen in liquid nitrogen. Pupae were stored at -80C until extraction. |
Collection Protocol Comments: | Samples were homogenized in ice cold 80% methanol and centrifuged. Supernatant was retained and stored at -80C until analysis. |
Sample Type: | whole fly |
Collection Method: | flash freezing in liquid nitrogen |
Collection Location: | Hahn Lab, 3137 Steinmetz |
Collection Frequency: | Daily |
Collection Time: | see treatments |
Volumeoramount Collected: | one pupa per sample |
Storage Conditions: | -80C |
Collection Vials: | 1.5 ml microcentrifuge tubes |
Storage Vials: | 1.5 ml microcentrifuge tubes |
Collection Tube Temp: | room temperature |