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MB Sample ID: SA076214
Local Sample ID: | U44_A2b |
Subject ID: | SU001162 |
Subject Type: | Invertebrate |
Subject Species: | Acropora cervicornis |
Taxonomy ID: | 6130 |
Genotype Strain: | U25, U41, U44 |
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Collection:
Collection ID: | CO001156 |
Collection Summary: | Coral colonies were brought to the surface intact, and ~3 cm nubbins were clipped from actively growing branch tips. Nubbins were placed in 20 mL scintillation vials containing 10 mL of 100% methanol spiked with a 0.005 mM aminoanthracene standard and immedately placed on ice. Samples were stored in a -20°C freezer overnight, then transported back to the laboratory n ice and again stored in a 20°C freezer overnight prior to extraction. The next day, samples were agitated for 5 minutes, then allowed to settle for one hour in the -20°C freezer. One mL of the sample extract was transferred to clean 1.5 mL microcentrifuge tubes and centrifuged at 20,000 g for 5 minutes. The supernatant was then transferred to a new 1.5 mL microcentrifuge tube and stored in a -80°C freezer until processing. |
Sample Type: | Tissue and skeleton |
Storage Conditions: | -80℃ |