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MB Sample ID: SA136737
Local Sample ID: | YGR023w-11 |
Subject ID: | SU001686 |
Subject Type: | Yeast |
Subject Species: | Saccharomyces cerevisiae |
Taxonomy ID: | 4932 |
Genotype Strain: | BY4742, YGR023w |
Cell Biosource Or Supplier: | Open Biosystems |
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Collection:
Collection ID: | CO001679 |
Collection Summary: | Cells were grown at 27°C, 210 rpm in 5 mL of complete synthetic medium (CSM, 0.67% Nitrogen base without amino acids and ammonium sulfate, 2% glucose). The next day, cells were replaced with fresh CSM and grow at 27°C to reach an OD of 0.6 - 0.7. 25 ml of each sample were harvested by centrifugation at 3,838 x g for 5 min at 4°C, washed with 1 ml ice cold sterile water, quenched using ice cold methanol, and stored at -80°C. |
Sample Type: | Yeast cells |