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MB Sample ID: SA155365
Local Sample ID: | s1003 |
Subject ID: | SU001760 |
Subject Type: | Mammal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Genotype Strain: | Swiss Webster |
Age Or Age Range: | 10-14 weeks |
Gender: | Male |
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Collection:
Collection ID: | CO001753 |
Collection Summary: | For all experiments, mice were euthanized by CO2 asphyxiation nine days (mono-colonization with Citrobacter portucalensis BEI HM-34 or Anaerostipes sp. BEI HM-220) or four weeks (all other experiments) following colonization, and four sample types (serum, urine, feces, and cecal contents) were harvested from each mouse. Prior to euthanization, urine and feces were collected. Whole blood was collected by cardiac puncture and serum was obtained using microcontainer serum separator tubes from Becton Dickinson following manufacturer’s instructions. The intact cecum was harvested and snap-frozen in liquid nitrogen. A single cecal sample was harvested for mono-association and conventional experiments, and three samples at three different sections of the cecum were harvested for the defined-community experiment. All mouse experiments were conducted under a protocol approved by the Stanford University Institutional Animal Care and Use Committee. |
Sample Type: | Feces;Urine;Serum;Cecal contents |