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MB Sample ID: SA175120
Local Sample ID: | 83_3p |
Subject ID: | SU001961 |
Subject Type: | Mammal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Genotype Strain: | Ndufs4, https://www.jax.org/strai n/02705 8 |
Age Or Age Range: | 45-50 days |
Gender: | Male |
Animal Animal Supplier: | Jackson Laboratory (ME, USA) |
Animal Light Cycle: | 12:12 h |
Animal Feed: | Rodent Breeder, Cat. #RM1845, LabChef, Nutritionhub |
Animal Water: | ad libitum |
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Collection:
Collection ID: | CO001954 |
Collection Summary: | Urine was collected from individual mice in metabolic cages after overnight (12 hours) fasting with ad libitum access to water. Since Ndufs4 KO mice yielded limited urine volumes, urine droplets that failed to reach the collection tube were also collected. Hence, the separating cone, collection funnel and urine collection tubes were all treated with sodium azide (NaN3; 0.1% w/v; WT: 100 μL and KO: 50 μL) to prevent bacterial growth. Samples were snap-frozen in liquid nitrogen upon collection and stored at −80 °C until used. |
Sample Type: | Urine |
Storage Conditions: | -80℃ |