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MB Sample ID: SA205794
Local Sample ID: | HT1080_ML210_03 |
Subject ID: | SU002231 |
Subject Type: | Cultured cells |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Cell Strain Details: | HT1080 |
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Collection:
Collection ID: | CO002224 |
Collection Summary: | Metabolites were extracted from cells in 35 mm culture plates by rapidly aspirating the culture medium and incubating the plates with 0.6 ml of an 80% methanol: 20% water mixture on a cold block on dry ice for 15 min. Next, the cell material was scraped into Eppendorf tubes pre-chilled on ice. After centrifugation at 13,000 RCF for 5 min at 4 °C, the supernatant was collected into a fresh tube and stored on dry ice until analysis. |
Sample Type: | Cultured cells |
Storage Conditions: | -80? |