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MB Sample ID: SA247700
Local Sample ID: | L_C3 |
Subject ID: | SU002562 |
Subject Type: | Bacteria |
Subject Species: | Veillonella parvula |
Taxonomy ID: | 29466 |
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Collection:
Collection ID: | CO002555 |
Collection Summary: | The strain Veillonella parvula SKV38 xdh::cat*. Briefly, V. parvula was grown on SK agar (composition: yeast extract 10 gL-1, casitone 10 gL-1, NaCl 2 gL-1, K2HPO4 0.4 gL-1) supplemented with either DL-lactate (50 mM lactate), potassium nitrate (40 mM KNO3), or both, incubated under anaerobic conditions at 37ÂșC overnight and then inoculated into the respective liquid media in biological triplicates. Cells and supernatants were harvested at mid-exponential phase (OD600=0.3), after centrifugation at 10,000g for 5 min. Both cell pellets and resulting supernatants were stored at -80C until processing for metabolite extraction and metabolomic analysis. A titration of cell pools and media pools was conducted (10,20,40,60,80,100%) and used to filter the data based on the correlation of the abundance of detected features with |
Sample Type: | Bacterial cells |