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MB Sample ID: SA094613
Local Sample ID: | HFD-BPA12 |
Subject ID: | SU001383 |
Subject Type: | Mammal |
Subject Species: | Rattus norvegicus |
Taxonomy ID: | 10116 |
Genotype Strain: | Sprague Dawley |
Age Or Age Range: | 240 days |
Gender: | Male |
Animal Animal Supplier: | Harlan |
Animal Housing: | polycarbonate-free caging |
Animal Light Cycle: | 14-hr light and 10-hr dark |
Animal Feed: | Phytoestrogen Reduced II 18-5 (Ziegler Bros, Inc) or D09100301 (Research Diets, Inc) |
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Combined analysis:
Analysis ID | AN002178 | AN002179 | AN002180 |
---|---|---|---|
Analysis type | MS | MS | MS |
Chromatography type | HILIC | HILIC | HILIC |
Chromatography system | Agilent 6495 QQQ | Agilent 6495 QQQ | Agilent 6495 QQQ |
Column | Waters XBridge Amide (100 x 4.6mm,3.5um) | Waters XBridge Amide (100 x 4.6mm,3.5um) | Phenomenex Luna NH2 (150 x 2.1mm,3um) |
MS Type | ESI | ESI | ESI |
MS instrument type | Triple quadrupole | Triple quadrupole | Triple quadrupole |
MS instrument name | Agilent 6495 QQQ | Agilent 6495 QQQ | Agilent 6495 QQQ |
Ion Mode | POSITIVE | NEGATIVE | POSITIVE |
Units | peak intensity | peak intensity | peak intensity |
MS:
MS ID: | MS002025 |
Analysis ID: | AN002178 |
Instrument Name: | Agilent 6495 QQQ |
Instrument Type: | Triple quadrupole |
MS Type: | ESI |
MS Comments: | For data acquisition through LC/MS analysis, 10 µL of suspended samples were injected and analyzed using a 6495 triple quadrupole mass spectrometer (Agilent Technologies, Santa Clara, CA) coupled to a HPLC system (Agilent Technologies, Santa Clara, CA) via Multiple reaction monitoring (MRM). Source parameters were as follows: Gas temperature- 250°C; Gas flow- 14 l/min; Nebulizer - 20psi; Sheath gas temperature - 350°C; Sheath gas flow- 12 l/min; Capillary - 3000 V positive and 3000 V negative; Nozzle voltage- 1500 V positive and 1500 V negative. Approximately 8–11 data points were acquired per detected metabolite. The data acquired using Agilent mass hunter software and data was analyzed using mass hunter quantitative analysis software. |
Ion Mode: | POSITIVE |
MS ID: | MS002026 |
Analysis ID: | AN002179 |
Instrument Name: | Agilent 6495 QQQ |
Instrument Type: | Triple quadrupole |
MS Type: | ESI |
MS Comments: | For data acquisition through LC/MS analysis, 10 µL of suspended samples were injected and analyzed using a 6495 triple quadrupole mass spectrometer (Agilent Technologies, Santa Clara, CA) coupled to a HPLC system (Agilent Technologies, Santa Clara, CA) via Multiple reaction monitoring (MRM). Source parameters were as follows: Gas temperature- 250°C; Gas flow- 14 l/min; Nebulizer - 20psi; Sheath gas temperature - 350°C; Sheath gas flow- 12 l/min; Capillary - 3000 V positive and 3000 V negative; Nozzle voltage- 1500 V positive and 1500 V negative. Approximately 8–11 data points were acquired per detected metabolite. The data acquired using Agilent mass hunter software and data was analyzed using mass hunter quantitative analysis software. |
Ion Mode: | NEGATIVE |
MS ID: | MS002027 |
Analysis ID: | AN002180 |
Instrument Name: | Agilent 6495 QQQ |
Instrument Type: | Triple quadrupole |
MS Type: | ESI |
MS Comments: | For data acquisition through LC/MS analysis, 10 µL of suspended samples were injected and analyzed using a 6495 triple quadrupole mass spectrometer (Agilent Technologies, Santa Clara, CA) coupled to a HPLC system (Agilent Technologies, Santa Clara, CA) via Multiple reaction monitoring (MRM). Source parameters were as follows: Gas temperature- 250°C; Gas flow- 14 l/min; Nebulizer - 20psi; Sheath gas temperature - 350°C; Sheath gas flow- 12 l/min; Capillary - 3000 V positive and 3000 V negative; Nozzle voltage- 1500 V positive and 1500 V negative. Approximately 8–11 data points were acquired per detected metabolite. The data acquired using Agilent mass hunter software and data was analyzed using mass hunter quantitative analysis software. |
Ion Mode: | POSITIVE |