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MB Sample ID: SA217627
Local Sample ID: | H_C_233 |
Subject ID: | SU002355 |
Subject Type: | Human |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Age Or Age Range: | 8.7-18.6 (year) |
Gender: | Male and female |
Human Ethnicity: | Korean |
Human Inclusion Criteria: | We recruited children and adolescent participants who visited the pediatric departments of the Hallym University Sacred Heart Hospital and Seoul National University Children’s Hospital from January 2019 to May 2020, after obtaining informed consent from the children and their parents. |
Human Exclusion Criteria: | We excluded participants who were taking alcohol or medications known to affect the results of liver function tests. Participants with viral hepatitis, such as hepatitis A, B, or C, or with Epstein–Barr virus, Wilson’s disease, autoimmune hepatitis, or muscular disease were also excluded. |
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Combined analysis:
Analysis ID | AN003706 | AN003707 | AN003708 | AN003709 |
---|---|---|---|---|
Analysis type | MS | MS | MS | MS |
Chromatography type | Reversed phase | Reversed phase | None (Direct infusion) | None (Direct infusion) |
Chromatography system | Thermo Dionex Ultimate 3000 | Thermo Dionex Ultimate 3000 | Thermo Dionex Ultimate 3000 | Thermo Dionex Ultimate 3000 |
Column | Biocrates AbsoluteIDQ p400 HR Kit UHPLC (PN 9120052121032) | Biocrates AbsoluteIDQ p400 HR Kit UHPLC (PN 9120052121032) | NA | NA |
MS Type | ESI | ESI | ESI | ESI |
MS instrument type | Orbitrap | Orbitrap | Orbitrap | Orbitrap |
MS instrument name | Thermo Q Exactive Plus Orbitrap | Thermo Q Exactive Plus Orbitrap | Thermo Q Exactive Plus Orbitrap | Thermo Q Exactive Plus Orbitrap |
Ion Mode | POSITIVE | POSITIVE | POSITIVE | POSITIVE |
Units | μM | μM | μM | μM |
MS:
MS ID: | MS003455 |
Analysis ID: | AN003706 |
Instrument Name: | Thermo Q Exactive Plus Orbitrap |
Instrument Type: | Orbitrap |
MS Type: | ESI |
MS Comments: | MS acquisition method, data processing method and software used for feature assignments are described in analysis protocol file attached. Please refer sections 10.3 (MS acquisition parameters) and 9 (data processing) of the method file attached. The final quantitative results were exported to micromolar values with pooled quality control sample normalization by median. Subsequently, values under the lower limit of detection were imputed by one-fifth of the minimum positive values of their corresponding variables. These data were used for further analysis. |
Ion Mode: | POSITIVE |
Acquisition Parameters File: | UM-p400-HR-Thermo-3.pdf |
Processing Parameters File: | UM-p400-HR-Thermo-3.pdf |
Analysis Protocol File: | UM-p400-HR-Thermo-3.pdf |
MS ID: | MS003456 |
Analysis ID: | AN003707 |
Instrument Name: | Thermo Q Exactive Plus Orbitrap |
Instrument Type: | Orbitrap |
MS Type: | ESI |
MS Comments: | MS acquisition method, data processing method and software used for feature assignments are described in analysis protocol file attached. Please refer sections 10.3 (MS acquisition parameters) and 9 (data processing) of the method file attached. The final quantitative results were exported to micromolar values with pooled quality control sample normalization by median. Subsequently, values under the lower limit of detection were imputed by one-fifth of the minimum positive values of their corresponding variables. These data were used for further analysis. |
Ion Mode: | POSITIVE |
Acquisition Parameters File: | UM-p400-HR-Thermo-3.pdf |
Processing Parameters File: | UM-p400-HR-Thermo-3.pdf |
Analysis Protocol File: | UM-p400-HR-Thermo-3.pdf |
MS ID: | MS003457 |
Analysis ID: | AN003708 |
Instrument Name: | Thermo Q Exactive Plus Orbitrap |
Instrument Type: | Orbitrap |
MS Type: | ESI |
MS Comments: | MS acquisition method, data processing method and software used for feature assignments are described in analysis protocol file attached. Please refer sections 10.3 (MS acquisition parameters) and 5.3 (data processing) of the method file attached. The final quantitative results were exported to micromolar values with pooled quality control sample normalization by median. Subsequently, values under the lower limit of detection were imputed by one-fifth of the minimum positive values of their corresponding variables. These data were used for further analysis. |
Ion Mode: | POSITIVE |
Acquisition Parameters File: | UM-p400-HR-Thermo-3.pdf |
Processing Parameters File: | UM-MetIDQ-Oxygen-9.pdf |
Analysis Protocol File: | UM-p400-HR-Thermo-3.pdf |
MS ID: | MS003458 |
Analysis ID: | AN003709 |
Instrument Name: | Thermo Q Exactive Plus Orbitrap |
Instrument Type: | Orbitrap |
MS Type: | ESI |
MS Comments: | MS acquisition method, data processing method and software used for feature assignments are described in analysis protocol file attached. Please refer sections 10.3 (MS acquisition parameters) and 5.3 (data processing) of the method file attached. The final quantitative results were exported to micromolar values with pooled quality control sample normalization by median. Subsequently, values under the lower limit of detection were imputed by one-fifth of the minimum positive values of their corresponding variables. These data were used for further analysis. |
Ion Mode: | POSITIVE |
Acquisition Parameters File: | UM-p400-HR-Thermo-3.pdf |
Processing Parameters File: | UM-MetIDQ-Oxygen-9.pdf |
Analysis Protocol File: | UM-p400-HR-Thermo-3.pdf |