Return to study ST000309 main page
MB Sample ID: SA014035
Local Sample ID: | MM66-2S1 |
Subject ID: | SU000329 |
Subject Type: | Bacterial cells |
Subject Species: | Staphylococcus aureus |
Taxonomy ID: | 1280 |
Species Group: | Microorganism |
Select appropriate tab below to view additional metadata details:
Sample Preparation:
Sampleprep ID: | SP000337 |
Sampleprep Summary: | The sample extraction was referred to the procedure of Roessner et al. (2000, 2001) with minor changes: About 10 mg (DW) of S. aureus cells were crashed in the mill (Model, Manufacturer), and then sonicated with the addition of 1.5 ml of 70% methanol for 10 min at 600C, followed by extraction with 1.5 ml water at room temperature. Both extracts were combined and dried in vacuum. Prior to GC/MS analysis, dried polar extracts were derivatized with 80 µl Methoxyamine hydrochloride (20mg ml-1) for 60 min at 400C and then with 80 µl MSTFA at 650C for 40 min. |