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MB Sample ID: SA061784
Local Sample ID: | cbn089_b2_9 |
Subject ID: | SU001038 |
Subject Type: | Other |
Subject Species: | Caenorhabditis elegans |
Taxonomy ID: | 6239 |
Age Or Age Range: | young adult |
Gender: | Hermaphrodite |
Species Group: | Recombinant inbred lines |
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Sample Preparation:
Sampleprep ID: | SP001045 |
Sampleprep Summary: | A synchronous population of young adult worms was washed off the plates in M9 buffer and the worm pellet was washed with dH2O for three times and then collected in a 2 mL Eppendorf tube and freeze-dried overnight. Dried worm pellets were stored at room temperature until use. A dry worm pellet was re-suspended in ice-cold 0.9% NaCl solution (250 µL). Worms were homogenized with a 5 mm steel bead using a TissueLyser II (Qiagen) for 2x2.5 min at frequency of 30 times/sec, followed by a tip sonication (energy level: 40 joule; output: 8 watts) for two times on ice water. Protein quantification was performed with BCA assay. |