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MB Sample ID: SA073570
Local Sample ID: | 25 |
Subject ID: | SU001133 |
Subject Type: | Mammal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Genotype Strain: | B6Ly5.2 |
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Sample Preparation:
Sampleprep ID: | SP001140 |
Sampleprep Summary: | To determine targeted taurine quantitation, samples (int estinal fecal content) from mice 21d post-transplant were harvested, homogenized, and snap-frozen in liquid N2. Fecal content was we ighed at necropsy. Samples were extracted with acetonitrile spiked with stable isotope-labeled C13 taurine standards. The supernatan t was analyzed by Agilent 6490 mass spectrometer in a positive electrospray ionization mode after liquid chromatography using a HILI C mode and isocratic gradient. Quantitation was performed by calibration to internal standards and expressed in mM after normalizati on for weights. |