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MB Sample ID: SA084187
Local Sample ID: | OZ277_u9h_a |
Subject ID: | SU001269 |
Subject Type: | Cultured cells |
Subject Species: | Plasmodium falciparum;Homo sapiens |
Taxonomy ID: | 5833;9606 |
Genotype Strain: | 3D7 |
Age Or Age Range: | 6-12 h post invasion |
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Sample Preparation:
Sampleprep ID: | SP001277 |
Sampleprep Summary: | Infected RBCs were adjusted to 10% parasitaemia and 2% haematocrit and the culture medium refreshed prior to drug addition. Following the drug incubation period, 2E8 cells were pelleted by centrifugation at 1,000 x g for 3 min and the culture medium was removed. Parasite metabolism was quenched by the addition of ice-cold PBS, pelleted again and the supernatant discarded prior to metabolite extraction. Metabolites were extracted from the cell pellet using 200 µL of cold chloroform/methanol/water (1:3:1). The extraction solvent containing the internal standard compounds CHAPS (3-[(3-cholamidopropyl)-dimethylammonio]-1-propanesulfonate), CAPS (3-(cyclohexylamino)-1-propanesulfonic acid), PIPES (1,4-piperazinediethanesulfonic acid) and TRIS (2-amino-2-(hydroxymethyl)-1,3-propanediol) was directly added to the cell pellet, mixed by pipetting and subjected to automatic vortex mixing for 1 h at 4°C. Following the 1 h incubation, samples were pelleted by centrifugation at 21,100 x g for 10 min, 110 µL of particle free supernatant was transferred to glass LC-MS vials and stored at -80°C until analysis. A 15 µL aliquot of each sample was combined to generate a pooled biological quality control (PBQC) sample. |
Processing Storage Conditions: | Described in summary |