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MB Sample ID: SA087424
Local Sample ID: | V474 |
Subject ID: | SU001298 |
Subject Type: | Human |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Age Or Age Range: | 4-8 months |
Gender: | Male and female |
Human Ethnicity: | Mainly Caucasian |
Human Trial Type: | RCT |
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Sample Preparation:
Sampleprep ID: | SP001306 |
Sampleprep Summary: | The sample preparation was done using the automated system MicroLab STAR® system from Hamilton Company. Prior to extraction, the samples were fortified with recovery standards for quality control (QC) purposes. Methanol was used to extract the metabolites and precipitate the proteins during vigorous shaking for 2 min (Glen Mills GenoGrinder 2000) followed by centrifugation. The resulting extract was divided into four aliquots to be subsequently analyzed by LC-MS/MS comprising of four platforms. Samples were placed on a TurboVap® (Zymark) to remove the organic solvent and stored overnight under nitrogen before preparation for LC-MS/MS analysis. |
Processing Storage Conditions: | -80℃ |