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MB Sample ID: SA099264
Local Sample ID: | Sample_8306 |
Subject ID: | SU001439 |
Subject Type: | Human |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Age Or Age Range: | 21-56 |
Gender: | Male and female |
Human Inclusion Criteria: | Cystic Fibrosis, adults |
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Sample Preparation:
Sampleprep ID: | SP001447 |
Sampleprep Summary: | The extraction solvent was chilled 1% DTT and spiked with 1.33 µM of both internal standards, linezolid-d3 and levofloxacin-d8. Solvent (150 µL) was added to 50 µL of sputum. The samples were vortexed for 30 seconds, shaken at 4˚C on a shaking platform with moderate agitation for 15 minutes, and centrifuged at 13,200 RCF for 10 minutes at 4˚C. The supernatant was pipetted into amber glass vials and injected directly into the LC-MS. |
Processing Storage Conditions: | On ice |
Extract Storage: | On ice |