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MB Sample ID: SA176675
Local Sample ID: | C08 |
Subject ID: | SU001984 |
Subject Type: | Human |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
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Sample Preparation:
Sampleprep ID: | SP001990 |
Sampleprep Summary: | At first, 50 µL of plasma was mixed with 150 µL of deaerated H2O containing 0.2 mg/mL of ribitol, which was used as an internal standard, (1200 rpm, 10 min, 4°C). Then, 800 µL of deaerated MeCN was added (1200 rpm, 10 min, 4°C) and centrifuged (16000×g, 3 min, 4°C). Next, 300 µL of supernatant were transferred to an Eppendorf tube. All the steps up to this point were performed in a cold room at 4°C. The samples were then dried in a vacuum centrifuge dryer for 1 hour and lyophilized overnight. For derivatization, first, 100 µL of methoxyamine hydrochloride in pyridine (20 mg/mL) was added to the samples, and the mixture was incubated (1200 rpm, 90 min, 30°C). A second derivatizing agent, N-methyl-N-trimethysilyl-trifuroacetamide (MSTFA), was then added and the mixture was incubated (1200 rpm, 30 min, 37°C). After centrifucation (16000×g, 3 min), 100 µL of derivatized samples were transferred to glass vials. |